MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis

The expression of hundreds of genes is induced by low temperatures via a cold signaling pathway. ICE1, a MYC-type transcription factor, plays an important role in the induction of CBF3/DREB1A to control cold-responsive genes and cold tolerance. To elucidate other molecular factors, a yeast 2-hybrid...

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Autores principales: Ohta, Masaru, Sato, Aiko, Renhu, Na, Yamamoto, Tsuyoshi, Oka, Nodoka, Zhu, Jian-Kang, Tada, Yasuomi, Suzaki, Takuya, Miura, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072781/
https://www.ncbi.nlm.nih.gov/pubmed/30072714
http://dx.doi.org/10.1038/s41598-018-29722-x
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author Ohta, Masaru
Sato, Aiko
Renhu, Na
Yamamoto, Tsuyoshi
Oka, Nodoka
Zhu, Jian-Kang
Tada, Yasuomi
Suzaki, Takuya
Miura, Kenji
author_facet Ohta, Masaru
Sato, Aiko
Renhu, Na
Yamamoto, Tsuyoshi
Oka, Nodoka
Zhu, Jian-Kang
Tada, Yasuomi
Suzaki, Takuya
Miura, Kenji
author_sort Ohta, Masaru
collection PubMed
description The expression of hundreds of genes is induced by low temperatures via a cold signaling pathway. ICE1, a MYC-type transcription factor, plays an important role in the induction of CBF3/DREB1A to control cold-responsive genes and cold tolerance. To elucidate other molecular factors, a yeast 2-hybrid screening was performed. Two MYC-type transcription factors, MYC67 and MYC70, were identified as ICE1-interacting proteins. The myc mutants were more tolerant to freezing temperatures than wild type. CBF3/DREB1A and other cold-responsive genes were up-regulated in the myc mutants. Overexpression of the MYC genes increased the cold sensitivity and down-regulated the expression of cold-responsive genes. The MYC proteins interacted with the cis-elements in the CBF3/DREB1A promoter, probably to interfere interaction between ICE1 and the cis-elements. Taken together, these results demonstrate that MYC67 and MYC70, ICE1 interactors, negatively regulate cold-responsive genes and cold tolerance.
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spelling pubmed-60727812018-08-07 MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis Ohta, Masaru Sato, Aiko Renhu, Na Yamamoto, Tsuyoshi Oka, Nodoka Zhu, Jian-Kang Tada, Yasuomi Suzaki, Takuya Miura, Kenji Sci Rep Article The expression of hundreds of genes is induced by low temperatures via a cold signaling pathway. ICE1, a MYC-type transcription factor, plays an important role in the induction of CBF3/DREB1A to control cold-responsive genes and cold tolerance. To elucidate other molecular factors, a yeast 2-hybrid screening was performed. Two MYC-type transcription factors, MYC67 and MYC70, were identified as ICE1-interacting proteins. The myc mutants were more tolerant to freezing temperatures than wild type. CBF3/DREB1A and other cold-responsive genes were up-regulated in the myc mutants. Overexpression of the MYC genes increased the cold sensitivity and down-regulated the expression of cold-responsive genes. The MYC proteins interacted with the cis-elements in the CBF3/DREB1A promoter, probably to interfere interaction between ICE1 and the cis-elements. Taken together, these results demonstrate that MYC67 and MYC70, ICE1 interactors, negatively regulate cold-responsive genes and cold tolerance. Nature Publishing Group UK 2018-08-02 /pmc/articles/PMC6072781/ /pubmed/30072714 http://dx.doi.org/10.1038/s41598-018-29722-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ohta, Masaru
Sato, Aiko
Renhu, Na
Yamamoto, Tsuyoshi
Oka, Nodoka
Zhu, Jian-Kang
Tada, Yasuomi
Suzaki, Takuya
Miura, Kenji
MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis
title MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis
title_full MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis
title_fullStr MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis
title_full_unstemmed MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis
title_short MYC-type transcription factors, MYC67 and MYC70, interact with ICE1 and negatively regulate cold tolerance in Arabidopsis
title_sort myc-type transcription factors, myc67 and myc70, interact with ice1 and negatively regulate cold tolerance in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072781/
https://www.ncbi.nlm.nih.gov/pubmed/30072714
http://dx.doi.org/10.1038/s41598-018-29722-x
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