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microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147
Colorectal cancer(CRC) is a prevalent malignancy in the world. There is growing evidence that microRNAs (miRNAs) as crucial modulator are in connection with many tumor-related diseases including CRC. Though miR-485-5p has been reported as an anti-oncogene in certain cancers, it remains unclear in CR...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072824/ https://www.ncbi.nlm.nih.gov/pubmed/30087700 http://dx.doi.org/10.7150/jca.24918 |
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author | Hu, Xiu-Xiu Xu, Xue-Ni He, Bang-Shun Sun, Hui-Ling Xu, Tao Liu, Xiang-Xiang Chen, Xiao-Xiang Zeng, Kai-Xuan Wang, Shu-Kui Pan, Yu-Qin |
author_facet | Hu, Xiu-Xiu Xu, Xue-Ni He, Bang-Shun Sun, Hui-Ling Xu, Tao Liu, Xiang-Xiang Chen, Xiao-Xiang Zeng, Kai-Xuan Wang, Shu-Kui Pan, Yu-Qin |
author_sort | Hu, Xiu-Xiu |
collection | PubMed |
description | Colorectal cancer(CRC) is a prevalent malignancy in the world. There is growing evidence that microRNAs (miRNAs) as crucial modulator are in connection with many tumor-related diseases including CRC. Though miR-485-5p has been reported as an anti-oncogene in certain cancers, it remains unclear in CRC. In this research, we found that miR-485-5p was at lower level expression in CRC tissues and cell lines compared to the paired paracancerous tissues and the normal colon epithelial cell line FHC, correspondingly. Furthermore, Experimental up-regulation miR-485-5p in DLD-1 and SW480 cells with mimic could inhibit the ability of proliferation, migration, invasion of CRC cell lines and facilitate cells apoptosis. Also, we confirmed that CD147 existed typically negative regulation by miR-485-5p through binding a conserved sequence specifically within the CD147 3′-untranslated region (3′UTR) and reintroduction of CD147 could rescue the phenotypic changes caused by miR-485-5p. The findings provide evidence to demonstrate the role of miR-485-5p/CD147 interaction in CRC and indicate that miR-485-5p might be exploited therapeutically in CRC. |
format | Online Article Text |
id | pubmed-6072824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-60728242018-08-07 microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147 Hu, Xiu-Xiu Xu, Xue-Ni He, Bang-Shun Sun, Hui-Ling Xu, Tao Liu, Xiang-Xiang Chen, Xiao-Xiang Zeng, Kai-Xuan Wang, Shu-Kui Pan, Yu-Qin J Cancer Research Paper Colorectal cancer(CRC) is a prevalent malignancy in the world. There is growing evidence that microRNAs (miRNAs) as crucial modulator are in connection with many tumor-related diseases including CRC. Though miR-485-5p has been reported as an anti-oncogene in certain cancers, it remains unclear in CRC. In this research, we found that miR-485-5p was at lower level expression in CRC tissues and cell lines compared to the paired paracancerous tissues and the normal colon epithelial cell line FHC, correspondingly. Furthermore, Experimental up-regulation miR-485-5p in DLD-1 and SW480 cells with mimic could inhibit the ability of proliferation, migration, invasion of CRC cell lines and facilitate cells apoptosis. Also, we confirmed that CD147 existed typically negative regulation by miR-485-5p through binding a conserved sequence specifically within the CD147 3′-untranslated region (3′UTR) and reintroduction of CD147 could rescue the phenotypic changes caused by miR-485-5p. The findings provide evidence to demonstrate the role of miR-485-5p/CD147 interaction in CRC and indicate that miR-485-5p might be exploited therapeutically in CRC. Ivyspring International Publisher 2018-06-23 /pmc/articles/PMC6072824/ /pubmed/30087700 http://dx.doi.org/10.7150/jca.24918 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Hu, Xiu-Xiu Xu, Xue-Ni He, Bang-Shun Sun, Hui-Ling Xu, Tao Liu, Xiang-Xiang Chen, Xiao-Xiang Zeng, Kai-Xuan Wang, Shu-Kui Pan, Yu-Qin microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147 |
title | microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147 |
title_full | microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147 |
title_fullStr | microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147 |
title_full_unstemmed | microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147 |
title_short | microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147 |
title_sort | microrna-485-5p functions as a tumor suppressor in colorectal cancer cells by targeting cd147 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072824/ https://www.ncbi.nlm.nih.gov/pubmed/30087700 http://dx.doi.org/10.7150/jca.24918 |
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