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Polyphenols Derived from Lychee Seed Suppress Aβ (1-42)-Induced Neuroinflammation
Amyloid-β (Aβ) is commonly recognized as the most important factor that results in neuronal cell death and accelerates the progression of Alzheimer’s disease (AD). Increasing evidence suggests that microglia activated by Aβ release an amount of neurotoxic inflammatory cytokines that contribute to ne...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073121/ https://www.ncbi.nlm.nih.gov/pubmed/30036972 http://dx.doi.org/10.3390/ijms19072109 |
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author | Tang, Yong Xiong, Rui Wu, An-Guo Yu, Chong-Lin Zhao, Ya Qiu, Wen-Qiao Wang, Xiu-Ling Teng, Jin-Feng Liu, Jian Chen, Hai-Xia Wu, Jian-Ming Qin, Da-Lian |
author_facet | Tang, Yong Xiong, Rui Wu, An-Guo Yu, Chong-Lin Zhao, Ya Qiu, Wen-Qiao Wang, Xiu-Ling Teng, Jin-Feng Liu, Jian Chen, Hai-Xia Wu, Jian-Ming Qin, Da-Lian |
author_sort | Tang, Yong |
collection | PubMed |
description | Amyloid-β (Aβ) is commonly recognized as the most important factor that results in neuronal cell death and accelerates the progression of Alzheimer’s disease (AD). Increasing evidence suggests that microglia activated by Aβ release an amount of neurotoxic inflammatory cytokines that contribute to neuron death and aggravate AD pathology. In our previous studies, we found that lychee seed fraction (LSF), an active fraction derived from the lychee seed, could significantly improve the cognitive function of AD rats and inhibit Aβ-induced neuroinflammation in vitro, and decrease neuronal injuries in vivo and in vitro. In the current study, we aimed to isolate and identify the specific components in LSF that were responsible for the anti-neuroinflammation effect using preparative high performance liquid chromatography (pre-HPLC), liquid chromatography-mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR) methods. To this end, we confirmed two polyphenols including catechin and procyanidin A2 that could improve the morphological status of BV-2 cells and suppress the release, mRNA levels, and protein expression of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) through downregulating the nuclear factor-κB (NF-κB) signaling pathway using ELISA, RT-PCR, and Western blotting methods. Furthermore, catechin and procyanidin A2 could inhibit Aβ-induced apoptosis in BV-2 cells by upregulating Bcl-2 and downregulating Bax protein expression. Therefore, the current study illustrated the active substances in lychee seed, and first reported that catechin and procyanidin A2 could suppress neuroinflammation in Aβ-induced BV-2 cells, which provides detailed insights into the molecular mechanism of catechin and procyanidin A2 in the neuroprotective effect, and their further validations of anti-neuroinflammation in vivo is also essential in future research. |
format | Online Article Text |
id | pubmed-6073121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60731212018-08-13 Polyphenols Derived from Lychee Seed Suppress Aβ (1-42)-Induced Neuroinflammation Tang, Yong Xiong, Rui Wu, An-Guo Yu, Chong-Lin Zhao, Ya Qiu, Wen-Qiao Wang, Xiu-Ling Teng, Jin-Feng Liu, Jian Chen, Hai-Xia Wu, Jian-Ming Qin, Da-Lian Int J Mol Sci Article Amyloid-β (Aβ) is commonly recognized as the most important factor that results in neuronal cell death and accelerates the progression of Alzheimer’s disease (AD). Increasing evidence suggests that microglia activated by Aβ release an amount of neurotoxic inflammatory cytokines that contribute to neuron death and aggravate AD pathology. In our previous studies, we found that lychee seed fraction (LSF), an active fraction derived from the lychee seed, could significantly improve the cognitive function of AD rats and inhibit Aβ-induced neuroinflammation in vitro, and decrease neuronal injuries in vivo and in vitro. In the current study, we aimed to isolate and identify the specific components in LSF that were responsible for the anti-neuroinflammation effect using preparative high performance liquid chromatography (pre-HPLC), liquid chromatography-mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR) methods. To this end, we confirmed two polyphenols including catechin and procyanidin A2 that could improve the morphological status of BV-2 cells and suppress the release, mRNA levels, and protein expression of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) through downregulating the nuclear factor-κB (NF-κB) signaling pathway using ELISA, RT-PCR, and Western blotting methods. Furthermore, catechin and procyanidin A2 could inhibit Aβ-induced apoptosis in BV-2 cells by upregulating Bcl-2 and downregulating Bax protein expression. Therefore, the current study illustrated the active substances in lychee seed, and first reported that catechin and procyanidin A2 could suppress neuroinflammation in Aβ-induced BV-2 cells, which provides detailed insights into the molecular mechanism of catechin and procyanidin A2 in the neuroprotective effect, and their further validations of anti-neuroinflammation in vivo is also essential in future research. MDPI 2018-07-20 /pmc/articles/PMC6073121/ /pubmed/30036972 http://dx.doi.org/10.3390/ijms19072109 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tang, Yong Xiong, Rui Wu, An-Guo Yu, Chong-Lin Zhao, Ya Qiu, Wen-Qiao Wang, Xiu-Ling Teng, Jin-Feng Liu, Jian Chen, Hai-Xia Wu, Jian-Ming Qin, Da-Lian Polyphenols Derived from Lychee Seed Suppress Aβ (1-42)-Induced Neuroinflammation |
title | Polyphenols Derived from Lychee Seed Suppress Aβ (1-42)-Induced Neuroinflammation |
title_full | Polyphenols Derived from Lychee Seed Suppress Aβ (1-42)-Induced Neuroinflammation |
title_fullStr | Polyphenols Derived from Lychee Seed Suppress Aβ (1-42)-Induced Neuroinflammation |
title_full_unstemmed | Polyphenols Derived from Lychee Seed Suppress Aβ (1-42)-Induced Neuroinflammation |
title_short | Polyphenols Derived from Lychee Seed Suppress Aβ (1-42)-Induced Neuroinflammation |
title_sort | polyphenols derived from lychee seed suppress aβ (1-42)-induced neuroinflammation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073121/ https://www.ncbi.nlm.nih.gov/pubmed/30036972 http://dx.doi.org/10.3390/ijms19072109 |
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