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Adducin‐1 is essential for spindle pole integrity through its interaction with TPX2
Bipolar spindle assembly is necessary to ensure the proper progression of cell division. Loss of spindle pole integrity leads to multipolar spindles and aberrant chromosomal segregation. However, the mechanism underlying the maintenance of spindle pole integrity remains unclear. In this study, we sh...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073210/ https://www.ncbi.nlm.nih.gov/pubmed/29925526 http://dx.doi.org/10.15252/embr.201745607 |
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author | Hsu, Wen‐Hsin Wang, Won‐Jing Lin, Wan‐Yi Huang, Yu‐Min Lai, Chien‐Chen Liao, Jung‐Chi Chen, Hong‐Chen |
author_facet | Hsu, Wen‐Hsin Wang, Won‐Jing Lin, Wan‐Yi Huang, Yu‐Min Lai, Chien‐Chen Liao, Jung‐Chi Chen, Hong‐Chen |
author_sort | Hsu, Wen‐Hsin |
collection | PubMed |
description | Bipolar spindle assembly is necessary to ensure the proper progression of cell division. Loss of spindle pole integrity leads to multipolar spindles and aberrant chromosomal segregation. However, the mechanism underlying the maintenance of spindle pole integrity remains unclear. In this study, we show that the actin‐binding protein adducin‐1 (ADD1) is phosphorylated at S726 during mitosis. S726‐phosphorylated ADD1 localizes to centrosomes, wherein it organizes into a rosette‐like structure at the pericentriolar material. ADD1 depletion causes centriole splitting and therefore results in multipolar spindles during mitosis, which can be restored by re‐expression of ADD1 and the phosphomimetic S726D mutant but not by the S726A mutant. Moreover, the phosphorylation of ADD1 at S726 is crucial for its interaction with TPX2, which is essential for spindle pole integrity. Together, our findings unveil a novel function of ADD1 in maintaining spindle pole integrity through its interaction with TPX2. |
format | Online Article Text |
id | pubmed-6073210 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60732102018-08-07 Adducin‐1 is essential for spindle pole integrity through its interaction with TPX2 Hsu, Wen‐Hsin Wang, Won‐Jing Lin, Wan‐Yi Huang, Yu‐Min Lai, Chien‐Chen Liao, Jung‐Chi Chen, Hong‐Chen EMBO Rep Articles Bipolar spindle assembly is necessary to ensure the proper progression of cell division. Loss of spindle pole integrity leads to multipolar spindles and aberrant chromosomal segregation. However, the mechanism underlying the maintenance of spindle pole integrity remains unclear. In this study, we show that the actin‐binding protein adducin‐1 (ADD1) is phosphorylated at S726 during mitosis. S726‐phosphorylated ADD1 localizes to centrosomes, wherein it organizes into a rosette‐like structure at the pericentriolar material. ADD1 depletion causes centriole splitting and therefore results in multipolar spindles during mitosis, which can be restored by re‐expression of ADD1 and the phosphomimetic S726D mutant but not by the S726A mutant. Moreover, the phosphorylation of ADD1 at S726 is crucial for its interaction with TPX2, which is essential for spindle pole integrity. Together, our findings unveil a novel function of ADD1 in maintaining spindle pole integrity through its interaction with TPX2. John Wiley and Sons Inc. 2018-06-19 2018-08 /pmc/articles/PMC6073210/ /pubmed/29925526 http://dx.doi.org/10.15252/embr.201745607 Text en © 2018 The Authors. Published under the terms of the CC BY 4.0 license This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Hsu, Wen‐Hsin Wang, Won‐Jing Lin, Wan‐Yi Huang, Yu‐Min Lai, Chien‐Chen Liao, Jung‐Chi Chen, Hong‐Chen Adducin‐1 is essential for spindle pole integrity through its interaction with TPX2 |
title | Adducin‐1 is essential for spindle pole integrity through its interaction with TPX2 |
title_full | Adducin‐1 is essential for spindle pole integrity through its interaction with TPX2 |
title_fullStr | Adducin‐1 is essential for spindle pole integrity through its interaction with TPX2 |
title_full_unstemmed | Adducin‐1 is essential for spindle pole integrity through its interaction with TPX2 |
title_short | Adducin‐1 is essential for spindle pole integrity through its interaction with TPX2 |
title_sort | adducin‐1 is essential for spindle pole integrity through its interaction with tpx2 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073210/ https://www.ncbi.nlm.nih.gov/pubmed/29925526 http://dx.doi.org/10.15252/embr.201745607 |
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