Cargando…
Antiobesity Effects of Ginsenoside Rg1 on 3T3-L1 Preadipocytes and High Fat Diet-Induced Obese Mice Mediated by AMPK
Ginsenosides Rg1 is one of the major pharmacologically active saponins in ginseng, which as an antioxidant reduces oxidative damage in the liver and can also be used to prevent cardiovascular diseases and diabetes. However, there is no research targeting the effect of lipid metabolism in high-fat di...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073290/ https://www.ncbi.nlm.nih.gov/pubmed/29954059 http://dx.doi.org/10.3390/nu10070830 |
_version_ | 1783344157548347392 |
---|---|
author | Liu, Huimin Wang, Jing Liu, Meihong Zhao, Hongyu Yaqoob, Sanabil Zheng, Mingzhu Cai, Dan Liu, Jingsheng |
author_facet | Liu, Huimin Wang, Jing Liu, Meihong Zhao, Hongyu Yaqoob, Sanabil Zheng, Mingzhu Cai, Dan Liu, Jingsheng |
author_sort | Liu, Huimin |
collection | PubMed |
description | Ginsenosides Rg1 is one of the major pharmacologically active saponins in ginseng, which as an antioxidant reduces oxidative damage in the liver and can also be used to prevent cardiovascular diseases and diabetes. However, there is no research targeting the effect of lipid metabolism in high-fat diet (HFD)-induced mice. In this study, we evaluated the anti-obesity effects of Rg1 in 3T3-L1 adipocyte cells and HFD-induced obese C57BL/6J mice. Administration of Rg1 to HFD-induced obese mice significantly decreased body weight, total cholesterol, and total triglyceride levels. In addition to effects in 3T3-L1 cells, Rg1 reduced the accumulation of lipid droplets in a dose-dependent manner. Furthermore, Rg1 exhibits an anti-adipogenic effect via regulation of the expression of the transcriptional factors and lipid metabolism-related genes in vivo and in vitro. We observed that Rg1 administration significantly increased the phosphorylation level of AMP-activated protein kinase (AMPK) in both epididymal white adipose tissue and 3T3-L1 cells. These results indicated that Rg1 works both in an anti-adipogenic and anti-obesity manner through inducing AMPK activation, inhibiting lipogenesis, and decreasing intracellular lipid content, adipocyte size, and adipose weight. |
format | Online Article Text |
id | pubmed-6073290 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60732902018-08-13 Antiobesity Effects of Ginsenoside Rg1 on 3T3-L1 Preadipocytes and High Fat Diet-Induced Obese Mice Mediated by AMPK Liu, Huimin Wang, Jing Liu, Meihong Zhao, Hongyu Yaqoob, Sanabil Zheng, Mingzhu Cai, Dan Liu, Jingsheng Nutrients Article Ginsenosides Rg1 is one of the major pharmacologically active saponins in ginseng, which as an antioxidant reduces oxidative damage in the liver and can also be used to prevent cardiovascular diseases and diabetes. However, there is no research targeting the effect of lipid metabolism in high-fat diet (HFD)-induced mice. In this study, we evaluated the anti-obesity effects of Rg1 in 3T3-L1 adipocyte cells and HFD-induced obese C57BL/6J mice. Administration of Rg1 to HFD-induced obese mice significantly decreased body weight, total cholesterol, and total triglyceride levels. In addition to effects in 3T3-L1 cells, Rg1 reduced the accumulation of lipid droplets in a dose-dependent manner. Furthermore, Rg1 exhibits an anti-adipogenic effect via regulation of the expression of the transcriptional factors and lipid metabolism-related genes in vivo and in vitro. We observed that Rg1 administration significantly increased the phosphorylation level of AMP-activated protein kinase (AMPK) in both epididymal white adipose tissue and 3T3-L1 cells. These results indicated that Rg1 works both in an anti-adipogenic and anti-obesity manner through inducing AMPK activation, inhibiting lipogenesis, and decreasing intracellular lipid content, adipocyte size, and adipose weight. MDPI 2018-06-27 /pmc/articles/PMC6073290/ /pubmed/29954059 http://dx.doi.org/10.3390/nu10070830 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Huimin Wang, Jing Liu, Meihong Zhao, Hongyu Yaqoob, Sanabil Zheng, Mingzhu Cai, Dan Liu, Jingsheng Antiobesity Effects of Ginsenoside Rg1 on 3T3-L1 Preadipocytes and High Fat Diet-Induced Obese Mice Mediated by AMPK |
title | Antiobesity Effects of Ginsenoside Rg1 on 3T3-L1 Preadipocytes and High Fat Diet-Induced Obese Mice Mediated by AMPK |
title_full | Antiobesity Effects of Ginsenoside Rg1 on 3T3-L1 Preadipocytes and High Fat Diet-Induced Obese Mice Mediated by AMPK |
title_fullStr | Antiobesity Effects of Ginsenoside Rg1 on 3T3-L1 Preadipocytes and High Fat Diet-Induced Obese Mice Mediated by AMPK |
title_full_unstemmed | Antiobesity Effects of Ginsenoside Rg1 on 3T3-L1 Preadipocytes and High Fat Diet-Induced Obese Mice Mediated by AMPK |
title_short | Antiobesity Effects of Ginsenoside Rg1 on 3T3-L1 Preadipocytes and High Fat Diet-Induced Obese Mice Mediated by AMPK |
title_sort | antiobesity effects of ginsenoside rg1 on 3t3-l1 preadipocytes and high fat diet-induced obese mice mediated by ampk |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073290/ https://www.ncbi.nlm.nih.gov/pubmed/29954059 http://dx.doi.org/10.3390/nu10070830 |
work_keys_str_mv | AT liuhuimin antiobesityeffectsofginsenosiderg1on3t3l1preadipocytesandhighfatdietinducedobesemicemediatedbyampk AT wangjing antiobesityeffectsofginsenosiderg1on3t3l1preadipocytesandhighfatdietinducedobesemicemediatedbyampk AT liumeihong antiobesityeffectsofginsenosiderg1on3t3l1preadipocytesandhighfatdietinducedobesemicemediatedbyampk AT zhaohongyu antiobesityeffectsofginsenosiderg1on3t3l1preadipocytesandhighfatdietinducedobesemicemediatedbyampk AT yaqoobsanabil antiobesityeffectsofginsenosiderg1on3t3l1preadipocytesandhighfatdietinducedobesemicemediatedbyampk AT zhengmingzhu antiobesityeffectsofginsenosiderg1on3t3l1preadipocytesandhighfatdietinducedobesemicemediatedbyampk AT caidan antiobesityeffectsofginsenosiderg1on3t3l1preadipocytesandhighfatdietinducedobesemicemediatedbyampk AT liujingsheng antiobesityeffectsofginsenosiderg1on3t3l1preadipocytesandhighfatdietinducedobesemicemediatedbyampk |