Cargando…

Diagnostic Performance of Kato Katz Technique and Point-of-Care Circulating Cathodic Antigen Rapid Test in Diagnosing Schistosoma mansoni Infection in HIV-1 Co-Infected Adults on the Shoreline of Lake Victoria, Tanzania

Background: The diagnostic performance of the Kato Katz (KK) technique and the point-of-care circulating cathodic antigen (POC-CCA) test in detecting S. mansoni infection in the presence of the human immunodeficiency virus-1 (HIV-1) infection has remained inconclusive. The present cross-sectional su...

Descripción completa

Detalles Bibliográficos
Autores principales: Mazigo, Humphrey D., Heukelbach, Jorg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073360/
https://www.ncbi.nlm.nih.gov/pubmed/30274450
http://dx.doi.org/10.3390/tropicalmed3020054
_version_ 1783344172329074688
author Mazigo, Humphrey D.
Heukelbach, Jorg
author_facet Mazigo, Humphrey D.
Heukelbach, Jorg
author_sort Mazigo, Humphrey D.
collection PubMed
description Background: The diagnostic performance of the Kato Katz (KK) technique and the point-of-care circulating cathodic antigen (POC-CCA) test in detecting S. mansoni infection in the presence of the human immunodeficiency virus-1 (HIV-1) infection has remained inconclusive. The present cross-sectional survey compared the diagnostic performance of the KK technique and the POC-CCA test in diagnosing S. mansoni infection in an adult population co-infected with HIV-1 in northwestern Tanzania. Methods: Single urine and stool samples from 979 adults were screened for S. mansoni infection using both the KK technique and POC-CCA tests. To compare the performance of the two diagnostic tests a combined artificial gold standard was created, based on either an egg-positive KK technique or a POC-CCA-positive test. Results: Based on the KK technique, the prevalence of S. mansoni was 47.3% (463/979, 95% CI: 44.2–50.4), as compared to 60.5% by the POC-CCA test (592/979; 95% CI: 57.4–63.5). The overall sensitivity and specificity of the POC-CCA test were 92.5% (95% CI: 89.4–94.9) and 73.3% (95% CI: 69.6–76.8), respectively. In the HIV-1 seropositive group, the sensitivity and specificity of the POC-CCA test were 78.1% (95% CI: 60.0–90.7) and 45.9% (95% CI: 35.8–56.3). Using a combined gold standard, the sensitivity of the POC-CCA test increased to >90% in both subgroups whereas that of the KK technique in the HIV-1 seropositive group was low (49.5%; 95% CI: 39.6–59.5). Conclusion: In the presence of HIV-1 co-infection, the KK technique attained a very low sensitivity. The POC-CCA test offers the best option for the rapid screening of S. mansoni infection in communities with a high prevalence of HIV-1 infection.
format Online
Article
Text
id pubmed-6073360
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-60733602018-09-24 Diagnostic Performance of Kato Katz Technique and Point-of-Care Circulating Cathodic Antigen Rapid Test in Diagnosing Schistosoma mansoni Infection in HIV-1 Co-Infected Adults on the Shoreline of Lake Victoria, Tanzania Mazigo, Humphrey D. Heukelbach, Jorg Trop Med Infect Dis Article Background: The diagnostic performance of the Kato Katz (KK) technique and the point-of-care circulating cathodic antigen (POC-CCA) test in detecting S. mansoni infection in the presence of the human immunodeficiency virus-1 (HIV-1) infection has remained inconclusive. The present cross-sectional survey compared the diagnostic performance of the KK technique and the POC-CCA test in diagnosing S. mansoni infection in an adult population co-infected with HIV-1 in northwestern Tanzania. Methods: Single urine and stool samples from 979 adults were screened for S. mansoni infection using both the KK technique and POC-CCA tests. To compare the performance of the two diagnostic tests a combined artificial gold standard was created, based on either an egg-positive KK technique or a POC-CCA-positive test. Results: Based on the KK technique, the prevalence of S. mansoni was 47.3% (463/979, 95% CI: 44.2–50.4), as compared to 60.5% by the POC-CCA test (592/979; 95% CI: 57.4–63.5). The overall sensitivity and specificity of the POC-CCA test were 92.5% (95% CI: 89.4–94.9) and 73.3% (95% CI: 69.6–76.8), respectively. In the HIV-1 seropositive group, the sensitivity and specificity of the POC-CCA test were 78.1% (95% CI: 60.0–90.7) and 45.9% (95% CI: 35.8–56.3). Using a combined gold standard, the sensitivity of the POC-CCA test increased to >90% in both subgroups whereas that of the KK technique in the HIV-1 seropositive group was low (49.5%; 95% CI: 39.6–59.5). Conclusion: In the presence of HIV-1 co-infection, the KK technique attained a very low sensitivity. The POC-CCA test offers the best option for the rapid screening of S. mansoni infection in communities with a high prevalence of HIV-1 infection. MDPI 2018-05-29 /pmc/articles/PMC6073360/ /pubmed/30274450 http://dx.doi.org/10.3390/tropicalmed3020054 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mazigo, Humphrey D.
Heukelbach, Jorg
Diagnostic Performance of Kato Katz Technique and Point-of-Care Circulating Cathodic Antigen Rapid Test in Diagnosing Schistosoma mansoni Infection in HIV-1 Co-Infected Adults on the Shoreline of Lake Victoria, Tanzania
title Diagnostic Performance of Kato Katz Technique and Point-of-Care Circulating Cathodic Antigen Rapid Test in Diagnosing Schistosoma mansoni Infection in HIV-1 Co-Infected Adults on the Shoreline of Lake Victoria, Tanzania
title_full Diagnostic Performance of Kato Katz Technique and Point-of-Care Circulating Cathodic Antigen Rapid Test in Diagnosing Schistosoma mansoni Infection in HIV-1 Co-Infected Adults on the Shoreline of Lake Victoria, Tanzania
title_fullStr Diagnostic Performance of Kato Katz Technique and Point-of-Care Circulating Cathodic Antigen Rapid Test in Diagnosing Schistosoma mansoni Infection in HIV-1 Co-Infected Adults on the Shoreline of Lake Victoria, Tanzania
title_full_unstemmed Diagnostic Performance of Kato Katz Technique and Point-of-Care Circulating Cathodic Antigen Rapid Test in Diagnosing Schistosoma mansoni Infection in HIV-1 Co-Infected Adults on the Shoreline of Lake Victoria, Tanzania
title_short Diagnostic Performance of Kato Katz Technique and Point-of-Care Circulating Cathodic Antigen Rapid Test in Diagnosing Schistosoma mansoni Infection in HIV-1 Co-Infected Adults on the Shoreline of Lake Victoria, Tanzania
title_sort diagnostic performance of kato katz technique and point-of-care circulating cathodic antigen rapid test in diagnosing schistosoma mansoni infection in hiv-1 co-infected adults on the shoreline of lake victoria, tanzania
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073360/
https://www.ncbi.nlm.nih.gov/pubmed/30274450
http://dx.doi.org/10.3390/tropicalmed3020054
work_keys_str_mv AT mazigohumphreyd diagnosticperformanceofkatokatztechniqueandpointofcarecirculatingcathodicantigenrapidtestindiagnosingschistosomamansoniinfectioninhiv1coinfectedadultsontheshorelineoflakevictoriatanzania
AT heukelbachjorg diagnosticperformanceofkatokatztechniqueandpointofcarecirculatingcathodicantigenrapidtestindiagnosingschistosomamansoniinfectioninhiv1coinfectedadultsontheshorelineoflakevictoriatanzania