Cargando…

Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway

Cerebral dopamine neurotrophic factor (CDNF), previously known as the conserved dopamine neurotrophic factor, belongs to the evolutionarily conserved CDNF/mesencephalic astrocyte-derived neurotrophic factor MANF family of neurotrophic factors that demonstrate neurotrophic activities in dopaminergic...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Geng-Lin, Wang, Li-Hong, Liu, Xing-Yu, Zhang, Ya-Xuan, Hu, Meng-Yang, Liu, Lin, Fang, Yuan-Yuan, Mu, Yu, Zhao, Yan, Huang, Shu-Hong, Liu, Ting, Wang, Xiao-Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073452/
https://www.ncbi.nlm.nih.gov/pubmed/29966219
http://dx.doi.org/10.3390/ijms19071905
_version_ 1783344193575321600
author Zhang, Geng-Lin
Wang, Li-Hong
Liu, Xing-Yu
Zhang, Ya-Xuan
Hu, Meng-Yang
Liu, Lin
Fang, Yuan-Yuan
Mu, Yu
Zhao, Yan
Huang, Shu-Hong
Liu, Ting
Wang, Xiao-Jing
author_facet Zhang, Geng-Lin
Wang, Li-Hong
Liu, Xing-Yu
Zhang, Ya-Xuan
Hu, Meng-Yang
Liu, Lin
Fang, Yuan-Yuan
Mu, Yu
Zhao, Yan
Huang, Shu-Hong
Liu, Ting
Wang, Xiao-Jing
author_sort Zhang, Geng-Lin
collection PubMed
description Cerebral dopamine neurotrophic factor (CDNF), previously known as the conserved dopamine neurotrophic factor, belongs to the evolutionarily conserved CDNF/mesencephalic astrocyte-derived neurotrophic factor MANF family of neurotrophic factors that demonstrate neurotrophic activities in dopaminergic neurons. The function of CDNF during brain ischemia is still not known. MANF is identified as an endoplasmic reticulum (ER) stress protein; however, the role of CDNF in ER stress remains to be fully elucidated. Here, we test the neuroprotective effect of CDNF on middle cerebral artery occlusion (MCAO) rats and neurons and astrocytes treated with oxygen–glucose depletion (OGD). We also investigate the expression of CDNF in cerebral ischemia and in primary neurons treated with ER stress-inducing agents. Our results show that CDNF can significantly reduce infarct volume, reduce apoptotic cells and improve motor function in MCAO rats, while CDNF can increase the cell viability of neurons and astrocytes treated by OGD. The expression of CDNF was upregulated in the peri-infarct tissue at 2 h of ischemia/24 h reperfusion. ER stress inducer can induce CDNF expression in primary cultured neurons. Our data indicate that CDNF has neuroprotective effects on cerebral ischemia and the OGD cell model and the protective mechanism of CDNF may occur through ER stress pathways.
format Online
Article
Text
id pubmed-6073452
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-60734522018-08-13 Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway Zhang, Geng-Lin Wang, Li-Hong Liu, Xing-Yu Zhang, Ya-Xuan Hu, Meng-Yang Liu, Lin Fang, Yuan-Yuan Mu, Yu Zhao, Yan Huang, Shu-Hong Liu, Ting Wang, Xiao-Jing Int J Mol Sci Article Cerebral dopamine neurotrophic factor (CDNF), previously known as the conserved dopamine neurotrophic factor, belongs to the evolutionarily conserved CDNF/mesencephalic astrocyte-derived neurotrophic factor MANF family of neurotrophic factors that demonstrate neurotrophic activities in dopaminergic neurons. The function of CDNF during brain ischemia is still not known. MANF is identified as an endoplasmic reticulum (ER) stress protein; however, the role of CDNF in ER stress remains to be fully elucidated. Here, we test the neuroprotective effect of CDNF on middle cerebral artery occlusion (MCAO) rats and neurons and astrocytes treated with oxygen–glucose depletion (OGD). We also investigate the expression of CDNF in cerebral ischemia and in primary neurons treated with ER stress-inducing agents. Our results show that CDNF can significantly reduce infarct volume, reduce apoptotic cells and improve motor function in MCAO rats, while CDNF can increase the cell viability of neurons and astrocytes treated by OGD. The expression of CDNF was upregulated in the peri-infarct tissue at 2 h of ischemia/24 h reperfusion. ER stress inducer can induce CDNF expression in primary cultured neurons. Our data indicate that CDNF has neuroprotective effects on cerebral ischemia and the OGD cell model and the protective mechanism of CDNF may occur through ER stress pathways. MDPI 2018-06-29 /pmc/articles/PMC6073452/ /pubmed/29966219 http://dx.doi.org/10.3390/ijms19071905 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Geng-Lin
Wang, Li-Hong
Liu, Xing-Yu
Zhang, Ya-Xuan
Hu, Meng-Yang
Liu, Lin
Fang, Yuan-Yuan
Mu, Yu
Zhao, Yan
Huang, Shu-Hong
Liu, Ting
Wang, Xiao-Jing
Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway
title Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway
title_full Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway
title_fullStr Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway
title_full_unstemmed Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway
title_short Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway
title_sort cerebral dopamine neurotrophic factor (cdnf) has neuroprotective effects against cerebral ischemia that may occur through the endoplasmic reticulum stress pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073452/
https://www.ncbi.nlm.nih.gov/pubmed/29966219
http://dx.doi.org/10.3390/ijms19071905
work_keys_str_mv AT zhanggenglin cerebraldopamineneurotrophicfactorcdnfhasneuroprotectiveeffectsagainstcerebralischemiathatmayoccurthroughtheendoplasmicreticulumstresspathway
AT wanglihong cerebraldopamineneurotrophicfactorcdnfhasneuroprotectiveeffectsagainstcerebralischemiathatmayoccurthroughtheendoplasmicreticulumstresspathway
AT liuxingyu cerebraldopamineneurotrophicfactorcdnfhasneuroprotectiveeffectsagainstcerebralischemiathatmayoccurthroughtheendoplasmicreticulumstresspathway
AT zhangyaxuan cerebraldopamineneurotrophicfactorcdnfhasneuroprotectiveeffectsagainstcerebralischemiathatmayoccurthroughtheendoplasmicreticulumstresspathway
AT humengyang cerebraldopamineneurotrophicfactorcdnfhasneuroprotectiveeffectsagainstcerebralischemiathatmayoccurthroughtheendoplasmicreticulumstresspathway
AT liulin cerebraldopamineneurotrophicfactorcdnfhasneuroprotectiveeffectsagainstcerebralischemiathatmayoccurthroughtheendoplasmicreticulumstresspathway
AT fangyuanyuan cerebraldopamineneurotrophicfactorcdnfhasneuroprotectiveeffectsagainstcerebralischemiathatmayoccurthroughtheendoplasmicreticulumstresspathway
AT muyu cerebraldopamineneurotrophicfactorcdnfhasneuroprotectiveeffectsagainstcerebralischemiathatmayoccurthroughtheendoplasmicreticulumstresspathway
AT zhaoyan cerebraldopamineneurotrophicfactorcdnfhasneuroprotectiveeffectsagainstcerebralischemiathatmayoccurthroughtheendoplasmicreticulumstresspathway
AT huangshuhong cerebraldopamineneurotrophicfactorcdnfhasneuroprotectiveeffectsagainstcerebralischemiathatmayoccurthroughtheendoplasmicreticulumstresspathway
AT liuting cerebraldopamineneurotrophicfactorcdnfhasneuroprotectiveeffectsagainstcerebralischemiathatmayoccurthroughtheendoplasmicreticulumstresspathway
AT wangxiaojing cerebraldopamineneurotrophicfactorcdnfhasneuroprotectiveeffectsagainstcerebralischemiathatmayoccurthroughtheendoplasmicreticulumstresspathway