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Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway
Cerebral dopamine neurotrophic factor (CDNF), previously known as the conserved dopamine neurotrophic factor, belongs to the evolutionarily conserved CDNF/mesencephalic astrocyte-derived neurotrophic factor MANF family of neurotrophic factors that demonstrate neurotrophic activities in dopaminergic...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073452/ https://www.ncbi.nlm.nih.gov/pubmed/29966219 http://dx.doi.org/10.3390/ijms19071905 |
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author | Zhang, Geng-Lin Wang, Li-Hong Liu, Xing-Yu Zhang, Ya-Xuan Hu, Meng-Yang Liu, Lin Fang, Yuan-Yuan Mu, Yu Zhao, Yan Huang, Shu-Hong Liu, Ting Wang, Xiao-Jing |
author_facet | Zhang, Geng-Lin Wang, Li-Hong Liu, Xing-Yu Zhang, Ya-Xuan Hu, Meng-Yang Liu, Lin Fang, Yuan-Yuan Mu, Yu Zhao, Yan Huang, Shu-Hong Liu, Ting Wang, Xiao-Jing |
author_sort | Zhang, Geng-Lin |
collection | PubMed |
description | Cerebral dopamine neurotrophic factor (CDNF), previously known as the conserved dopamine neurotrophic factor, belongs to the evolutionarily conserved CDNF/mesencephalic astrocyte-derived neurotrophic factor MANF family of neurotrophic factors that demonstrate neurotrophic activities in dopaminergic neurons. The function of CDNF during brain ischemia is still not known. MANF is identified as an endoplasmic reticulum (ER) stress protein; however, the role of CDNF in ER stress remains to be fully elucidated. Here, we test the neuroprotective effect of CDNF on middle cerebral artery occlusion (MCAO) rats and neurons and astrocytes treated with oxygen–glucose depletion (OGD). We also investigate the expression of CDNF in cerebral ischemia and in primary neurons treated with ER stress-inducing agents. Our results show that CDNF can significantly reduce infarct volume, reduce apoptotic cells and improve motor function in MCAO rats, while CDNF can increase the cell viability of neurons and astrocytes treated by OGD. The expression of CDNF was upregulated in the peri-infarct tissue at 2 h of ischemia/24 h reperfusion. ER stress inducer can induce CDNF expression in primary cultured neurons. Our data indicate that CDNF has neuroprotective effects on cerebral ischemia and the OGD cell model and the protective mechanism of CDNF may occur through ER stress pathways. |
format | Online Article Text |
id | pubmed-6073452 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60734522018-08-13 Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway Zhang, Geng-Lin Wang, Li-Hong Liu, Xing-Yu Zhang, Ya-Xuan Hu, Meng-Yang Liu, Lin Fang, Yuan-Yuan Mu, Yu Zhao, Yan Huang, Shu-Hong Liu, Ting Wang, Xiao-Jing Int J Mol Sci Article Cerebral dopamine neurotrophic factor (CDNF), previously known as the conserved dopamine neurotrophic factor, belongs to the evolutionarily conserved CDNF/mesencephalic astrocyte-derived neurotrophic factor MANF family of neurotrophic factors that demonstrate neurotrophic activities in dopaminergic neurons. The function of CDNF during brain ischemia is still not known. MANF is identified as an endoplasmic reticulum (ER) stress protein; however, the role of CDNF in ER stress remains to be fully elucidated. Here, we test the neuroprotective effect of CDNF on middle cerebral artery occlusion (MCAO) rats and neurons and astrocytes treated with oxygen–glucose depletion (OGD). We also investigate the expression of CDNF in cerebral ischemia and in primary neurons treated with ER stress-inducing agents. Our results show that CDNF can significantly reduce infarct volume, reduce apoptotic cells and improve motor function in MCAO rats, while CDNF can increase the cell viability of neurons and astrocytes treated by OGD. The expression of CDNF was upregulated in the peri-infarct tissue at 2 h of ischemia/24 h reperfusion. ER stress inducer can induce CDNF expression in primary cultured neurons. Our data indicate that CDNF has neuroprotective effects on cerebral ischemia and the OGD cell model and the protective mechanism of CDNF may occur through ER stress pathways. MDPI 2018-06-29 /pmc/articles/PMC6073452/ /pubmed/29966219 http://dx.doi.org/10.3390/ijms19071905 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Geng-Lin Wang, Li-Hong Liu, Xing-Yu Zhang, Ya-Xuan Hu, Meng-Yang Liu, Lin Fang, Yuan-Yuan Mu, Yu Zhao, Yan Huang, Shu-Hong Liu, Ting Wang, Xiao-Jing Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway |
title | Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway |
title_full | Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway |
title_fullStr | Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway |
title_full_unstemmed | Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway |
title_short | Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway |
title_sort | cerebral dopamine neurotrophic factor (cdnf) has neuroprotective effects against cerebral ischemia that may occur through the endoplasmic reticulum stress pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073452/ https://www.ncbi.nlm.nih.gov/pubmed/29966219 http://dx.doi.org/10.3390/ijms19071905 |
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