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Comparative Analysis between the In Vivo Biodistribution and Therapeutic Efficacy of Adipose-Derived Mesenchymal Stromal Cells Administered Intraperitoneally in Experimental Colitis

Mesenchymal stem cells (MSCs) have emerged as a promising treatment for inflammatory diseases. The immunomodulatory effect of MSCs takes place both by direct cell-to-cell contact and by means of soluble factors that leads to an increased accumulation of regulatory immune cells at the sites of inflam...

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Autores principales: Lopez-Santalla, Mercedes, Mancheño-Corvo, Pablo, Escolano, Amelia, Menta, Ramon, Delarosa, Olga, Redondo, Juan M., Bueren, Juan A., Dalemans, Wilfried, Lombardo, Eleuterio, Garin, Marina I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073850/
https://www.ncbi.nlm.nih.gov/pubmed/29937494
http://dx.doi.org/10.3390/ijms19071853
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author Lopez-Santalla, Mercedes
Mancheño-Corvo, Pablo
Escolano, Amelia
Menta, Ramon
Delarosa, Olga
Redondo, Juan M.
Bueren, Juan A.
Dalemans, Wilfried
Lombardo, Eleuterio
Garin, Marina I.
author_facet Lopez-Santalla, Mercedes
Mancheño-Corvo, Pablo
Escolano, Amelia
Menta, Ramon
Delarosa, Olga
Redondo, Juan M.
Bueren, Juan A.
Dalemans, Wilfried
Lombardo, Eleuterio
Garin, Marina I.
author_sort Lopez-Santalla, Mercedes
collection PubMed
description Mesenchymal stem cells (MSCs) have emerged as a promising treatment for inflammatory diseases. The immunomodulatory effect of MSCs takes place both by direct cell-to-cell contact and by means of soluble factors that leads to an increased accumulation of regulatory immune cells at the sites of inflammation. Similar efficacy of MSCs has been described regardless of the route of administration used, the inflammation conditions and the major histocompatibility complex context. These observations raise the question of whether the migration of the MSCs to the inflamed tissues is a pre-requisite to achieve their beneficial effect. To address this, we examined the biodistribution and the efficacy of intraperitoneal luciferase-expressing human expanded adipose-derived stem cells (Luci-eASCs) in a mouse model of colitis. Luci-eASC-infused mice were stratified according to their response to the Luci-eASC treatment. According to the stratification criteria, there was a tendency to increase the bioluminescence signal in the intestine at the expense of a decrease in the bioluminescence signal in the liver in the “responder” mice. These data thus suggest that the accumulation of the eASCs to the inflamed tissues is beneficial for achieving an optimal modulation of inflammation.
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spelling pubmed-60738502018-08-13 Comparative Analysis between the In Vivo Biodistribution and Therapeutic Efficacy of Adipose-Derived Mesenchymal Stromal Cells Administered Intraperitoneally in Experimental Colitis Lopez-Santalla, Mercedes Mancheño-Corvo, Pablo Escolano, Amelia Menta, Ramon Delarosa, Olga Redondo, Juan M. Bueren, Juan A. Dalemans, Wilfried Lombardo, Eleuterio Garin, Marina I. Int J Mol Sci Article Mesenchymal stem cells (MSCs) have emerged as a promising treatment for inflammatory diseases. The immunomodulatory effect of MSCs takes place both by direct cell-to-cell contact and by means of soluble factors that leads to an increased accumulation of regulatory immune cells at the sites of inflammation. Similar efficacy of MSCs has been described regardless of the route of administration used, the inflammation conditions and the major histocompatibility complex context. These observations raise the question of whether the migration of the MSCs to the inflamed tissues is a pre-requisite to achieve their beneficial effect. To address this, we examined the biodistribution and the efficacy of intraperitoneal luciferase-expressing human expanded adipose-derived stem cells (Luci-eASCs) in a mouse model of colitis. Luci-eASC-infused mice were stratified according to their response to the Luci-eASC treatment. According to the stratification criteria, there was a tendency to increase the bioluminescence signal in the intestine at the expense of a decrease in the bioluminescence signal in the liver in the “responder” mice. These data thus suggest that the accumulation of the eASCs to the inflamed tissues is beneficial for achieving an optimal modulation of inflammation. MDPI 2018-06-23 /pmc/articles/PMC6073850/ /pubmed/29937494 http://dx.doi.org/10.3390/ijms19071853 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lopez-Santalla, Mercedes
Mancheño-Corvo, Pablo
Escolano, Amelia
Menta, Ramon
Delarosa, Olga
Redondo, Juan M.
Bueren, Juan A.
Dalemans, Wilfried
Lombardo, Eleuterio
Garin, Marina I.
Comparative Analysis between the In Vivo Biodistribution and Therapeutic Efficacy of Adipose-Derived Mesenchymal Stromal Cells Administered Intraperitoneally in Experimental Colitis
title Comparative Analysis between the In Vivo Biodistribution and Therapeutic Efficacy of Adipose-Derived Mesenchymal Stromal Cells Administered Intraperitoneally in Experimental Colitis
title_full Comparative Analysis between the In Vivo Biodistribution and Therapeutic Efficacy of Adipose-Derived Mesenchymal Stromal Cells Administered Intraperitoneally in Experimental Colitis
title_fullStr Comparative Analysis between the In Vivo Biodistribution and Therapeutic Efficacy of Adipose-Derived Mesenchymal Stromal Cells Administered Intraperitoneally in Experimental Colitis
title_full_unstemmed Comparative Analysis between the In Vivo Biodistribution and Therapeutic Efficacy of Adipose-Derived Mesenchymal Stromal Cells Administered Intraperitoneally in Experimental Colitis
title_short Comparative Analysis between the In Vivo Biodistribution and Therapeutic Efficacy of Adipose-Derived Mesenchymal Stromal Cells Administered Intraperitoneally in Experimental Colitis
title_sort comparative analysis between the in vivo biodistribution and therapeutic efficacy of adipose-derived mesenchymal stromal cells administered intraperitoneally in experimental colitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073850/
https://www.ncbi.nlm.nih.gov/pubmed/29937494
http://dx.doi.org/10.3390/ijms19071853
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