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Isolation of Adipose-Derived Stem/Stromal Cells from Cryopreserved Fat Tissue and Transplantation into Rats with Spinal Cord Injury

Adipose tissue contains multipotent cells known as adipose-derived stem/stromal cells (ASCs), which have therapeutic potential for various diseases. Although the demand for adipose tissue for research use remains high, no adipose tissue bank exists. In this study, we attempted to isolate ASCs from c...

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Autores principales: Ohta, Yuki, Takenaga, Mitsuko, Hamaguchi, Akemi, Ootaki, Masanori, Takeba, Yuko, Kobayashi, Tsukasa, Watanabe, Minoru, Iiri, Taroh, Matsumoto, Naoki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073880/
https://www.ncbi.nlm.nih.gov/pubmed/29976859
http://dx.doi.org/10.3390/ijms19071963
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author Ohta, Yuki
Takenaga, Mitsuko
Hamaguchi, Akemi
Ootaki, Masanori
Takeba, Yuko
Kobayashi, Tsukasa
Watanabe, Minoru
Iiri, Taroh
Matsumoto, Naoki
author_facet Ohta, Yuki
Takenaga, Mitsuko
Hamaguchi, Akemi
Ootaki, Masanori
Takeba, Yuko
Kobayashi, Tsukasa
Watanabe, Minoru
Iiri, Taroh
Matsumoto, Naoki
author_sort Ohta, Yuki
collection PubMed
description Adipose tissue contains multipotent cells known as adipose-derived stem/stromal cells (ASCs), which have therapeutic potential for various diseases. Although the demand for adipose tissue for research use remains high, no adipose tissue bank exists. In this study, we attempted to isolate ASCs from cryopreserved adipose tissue with the aim of developing a banking system. ASCs were isolated from fresh and cryopreserved adipose tissue of rats and compared for proliferation (doubling time), differentiation capability (adipocytes), and cytokine (hepatocyte growth factor and vascular endothelial growth factor) secretion. Finally, ASCs (2.5 × 10(6)) were intravenously infused into rats with spinal cord injury, after which hindlimb motor function was evaluated. Isolation and culture of ASCs from cryopreserved adipose tissue were possible, and their characteristics were not significantly different from those of fresh tissue. Transplantation of ASCs derived from cryopreserved tissue significantly promoted restoration of hindlimb movement function in injured model rats. These results indicate that cryopreservation of adipose tissue may be an option for clinical application.
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spelling pubmed-60738802018-08-13 Isolation of Adipose-Derived Stem/Stromal Cells from Cryopreserved Fat Tissue and Transplantation into Rats with Spinal Cord Injury Ohta, Yuki Takenaga, Mitsuko Hamaguchi, Akemi Ootaki, Masanori Takeba, Yuko Kobayashi, Tsukasa Watanabe, Minoru Iiri, Taroh Matsumoto, Naoki Int J Mol Sci Article Adipose tissue contains multipotent cells known as adipose-derived stem/stromal cells (ASCs), which have therapeutic potential for various diseases. Although the demand for adipose tissue for research use remains high, no adipose tissue bank exists. In this study, we attempted to isolate ASCs from cryopreserved adipose tissue with the aim of developing a banking system. ASCs were isolated from fresh and cryopreserved adipose tissue of rats and compared for proliferation (doubling time), differentiation capability (adipocytes), and cytokine (hepatocyte growth factor and vascular endothelial growth factor) secretion. Finally, ASCs (2.5 × 10(6)) were intravenously infused into rats with spinal cord injury, after which hindlimb motor function was evaluated. Isolation and culture of ASCs from cryopreserved adipose tissue were possible, and their characteristics were not significantly different from those of fresh tissue. Transplantation of ASCs derived from cryopreserved tissue significantly promoted restoration of hindlimb movement function in injured model rats. These results indicate that cryopreservation of adipose tissue may be an option for clinical application. MDPI 2018-07-05 /pmc/articles/PMC6073880/ /pubmed/29976859 http://dx.doi.org/10.3390/ijms19071963 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ohta, Yuki
Takenaga, Mitsuko
Hamaguchi, Akemi
Ootaki, Masanori
Takeba, Yuko
Kobayashi, Tsukasa
Watanabe, Minoru
Iiri, Taroh
Matsumoto, Naoki
Isolation of Adipose-Derived Stem/Stromal Cells from Cryopreserved Fat Tissue and Transplantation into Rats with Spinal Cord Injury
title Isolation of Adipose-Derived Stem/Stromal Cells from Cryopreserved Fat Tissue and Transplantation into Rats with Spinal Cord Injury
title_full Isolation of Adipose-Derived Stem/Stromal Cells from Cryopreserved Fat Tissue and Transplantation into Rats with Spinal Cord Injury
title_fullStr Isolation of Adipose-Derived Stem/Stromal Cells from Cryopreserved Fat Tissue and Transplantation into Rats with Spinal Cord Injury
title_full_unstemmed Isolation of Adipose-Derived Stem/Stromal Cells from Cryopreserved Fat Tissue and Transplantation into Rats with Spinal Cord Injury
title_short Isolation of Adipose-Derived Stem/Stromal Cells from Cryopreserved Fat Tissue and Transplantation into Rats with Spinal Cord Injury
title_sort isolation of adipose-derived stem/stromal cells from cryopreserved fat tissue and transplantation into rats with spinal cord injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073880/
https://www.ncbi.nlm.nih.gov/pubmed/29976859
http://dx.doi.org/10.3390/ijms19071963
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