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A protocol for introduction of multiple genetic modifications in Saccharomyces cerevisiae using CRISPR/Cas9

Here, two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method us...

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Detalles Bibliográficos
Autores principales:	Mans, Robert, Wijsman, Melanie, Daran-Lapujade, Pascale, Daran, Jean-Marc
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2018
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6074844/ https://www.ncbi.nlm.nih.gov/pubmed/29860374 http://dx.doi.org/10.1093/femsyr/foy063

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