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SCAMP4 enhances the senescent cell secretome
The senescence-associated secretory phenotype (SASP) is a major trait of senescent cells, but the molecular regulators of SASP factor secretion are poorly understood. Mass spectrometry analysis revealed that secretory carrier membrane protein 4 (SCAMP4) levels were strikingly elevated on the surface...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6075036/ https://www.ncbi.nlm.nih.gov/pubmed/29967290 http://dx.doi.org/10.1101/gad.313270.118 |
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author | Kim, Kyoung Mi Noh, Ji Heon Bodogai, Monica Martindale, Jennifer L. Pandey, Poonam R. Yang, Xiaoling Biragyn, Arya Abdelmohsen, Kotb Gorospe, Myriam |
author_facet | Kim, Kyoung Mi Noh, Ji Heon Bodogai, Monica Martindale, Jennifer L. Pandey, Poonam R. Yang, Xiaoling Biragyn, Arya Abdelmohsen, Kotb Gorospe, Myriam |
author_sort | Kim, Kyoung Mi |
collection | PubMed |
description | The senescence-associated secretory phenotype (SASP) is a major trait of senescent cells, but the molecular regulators of SASP factor secretion are poorly understood. Mass spectrometry analysis revealed that secretory carrier membrane protein 4 (SCAMP4) levels were strikingly elevated on the surface of senescent cells compared with proliferating cells. Interestingly, silencing SCAMP4 in senescent fibroblasts reduced the secretion of SASP factors, including interleukin 6 (IL6), IL8, growth differentiation factor 15 (GDF-15), C-X-C motif chemokine ligand 1 (CXCL1), and IL7, while, conversely, SCAMP4 overexpression in proliferating fibroblasts increased SASP factor secretion. Our results indicate that SCAMP4 accumulates on the surface of senescent cells, promotes SASP factor secretion, and critically enhances the SASP phenotype. |
format | Online Article Text |
id | pubmed-6075036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-60750362019-01-01 SCAMP4 enhances the senescent cell secretome Kim, Kyoung Mi Noh, Ji Heon Bodogai, Monica Martindale, Jennifer L. Pandey, Poonam R. Yang, Xiaoling Biragyn, Arya Abdelmohsen, Kotb Gorospe, Myriam Genes Dev Research Communication The senescence-associated secretory phenotype (SASP) is a major trait of senescent cells, but the molecular regulators of SASP factor secretion are poorly understood. Mass spectrometry analysis revealed that secretory carrier membrane protein 4 (SCAMP4) levels were strikingly elevated on the surface of senescent cells compared with proliferating cells. Interestingly, silencing SCAMP4 in senescent fibroblasts reduced the secretion of SASP factors, including interleukin 6 (IL6), IL8, growth differentiation factor 15 (GDF-15), C-X-C motif chemokine ligand 1 (CXCL1), and IL7, while, conversely, SCAMP4 overexpression in proliferating fibroblasts increased SASP factor secretion. Our results indicate that SCAMP4 accumulates on the surface of senescent cells, promotes SASP factor secretion, and critically enhances the SASP phenotype. Cold Spring Harbor Laboratory Press 2018-07-01 /pmc/articles/PMC6075036/ /pubmed/29967290 http://dx.doi.org/10.1101/gad.313270.118 Text en Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This is a work of the US Government. |
spellingShingle | Research Communication Kim, Kyoung Mi Noh, Ji Heon Bodogai, Monica Martindale, Jennifer L. Pandey, Poonam R. Yang, Xiaoling Biragyn, Arya Abdelmohsen, Kotb Gorospe, Myriam SCAMP4 enhances the senescent cell secretome |
title | SCAMP4 enhances the senescent cell secretome |
title_full | SCAMP4 enhances the senescent cell secretome |
title_fullStr | SCAMP4 enhances the senescent cell secretome |
title_full_unstemmed | SCAMP4 enhances the senescent cell secretome |
title_short | SCAMP4 enhances the senescent cell secretome |
title_sort | scamp4 enhances the senescent cell secretome |
topic | Research Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6075036/ https://www.ncbi.nlm.nih.gov/pubmed/29967290 http://dx.doi.org/10.1101/gad.313270.118 |
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