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Cumulus cell-released tumor necrosis factor (TNF)-α promotes post-ovulatory aging of mouse oocytes

Although previous studies indicated that cumulus cells (CCs) accelerate oocyte aging by releasing soluble factors, the factors have yet to be characterized. While demonstrating that CCs promoted oocyte aging by releasing soluble Fas ligand (sFasL), our recent study suggested that CCs might secrete o...

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Autores principales: Kong, Qiao-Qiao, Wang, Jia, Xiao, Bin, Lin, Fei-Hu, Zhu, Jiang, Sun, Guang-Yi, Luo, Ming-Jiu, Tan, Jing-He
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6075436/
https://www.ncbi.nlm.nih.gov/pubmed/30048240
http://dx.doi.org/10.18632/aging.101507
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author Kong, Qiao-Qiao
Wang, Jia
Xiao, Bin
Lin, Fei-Hu
Zhu, Jiang
Sun, Guang-Yi
Luo, Ming-Jiu
Tan, Jing-He
author_facet Kong, Qiao-Qiao
Wang, Jia
Xiao, Bin
Lin, Fei-Hu
Zhu, Jiang
Sun, Guang-Yi
Luo, Ming-Jiu
Tan, Jing-He
author_sort Kong, Qiao-Qiao
collection PubMed
description Although previous studies indicated that cumulus cells (CCs) accelerate oocyte aging by releasing soluble factors, the factors have yet to be characterized. While demonstrating that CCs promoted oocyte aging by releasing soluble Fas ligand (sFasL), our recent study suggested that CCs might secrete other factors to mediate oocyte aging as well. This study tested whether CCs accelerate oocyte aging by secreting tumor necrosis factor (TNF)-α. The results showed that mouse CCs undergoing apoptosis released soluble TNF-α (sTNF-α) during in vitro aging. While ethanol activation rates were higher, the maturation-promoting factor (MPF) activity was lower significantly after culture of cumulus-denuded oocytes (DOs) in medium conditioned with CCs for 36 h than in medium conditioned for 24 h. Aging mouse oocytes expressed TNF-receptor 1. The CCs released equal amounts of sTNF-α and sFasL during aging in vitro, and the TNF-α-knockdown CCs secreted less sFasL than the control CCs did. Treatment of DOs in vitro with sTNF-α significantly accelerated their aging. The aging-promoting effect of sTNF-α was significantly reduced in TNF-α-knocked-down CCs and in CCs from the TNF-α-knockout mice. It is concluded that mouse CCs accelerate oocyte aging by secreting sTNF-α as well as sFasL.
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spelling pubmed-60754362018-08-06 Cumulus cell-released tumor necrosis factor (TNF)-α promotes post-ovulatory aging of mouse oocytes Kong, Qiao-Qiao Wang, Jia Xiao, Bin Lin, Fei-Hu Zhu, Jiang Sun, Guang-Yi Luo, Ming-Jiu Tan, Jing-He Aging (Albany NY) Research Paper Although previous studies indicated that cumulus cells (CCs) accelerate oocyte aging by releasing soluble factors, the factors have yet to be characterized. While demonstrating that CCs promoted oocyte aging by releasing soluble Fas ligand (sFasL), our recent study suggested that CCs might secrete other factors to mediate oocyte aging as well. This study tested whether CCs accelerate oocyte aging by secreting tumor necrosis factor (TNF)-α. The results showed that mouse CCs undergoing apoptosis released soluble TNF-α (sTNF-α) during in vitro aging. While ethanol activation rates were higher, the maturation-promoting factor (MPF) activity was lower significantly after culture of cumulus-denuded oocytes (DOs) in medium conditioned with CCs for 36 h than in medium conditioned for 24 h. Aging mouse oocytes expressed TNF-receptor 1. The CCs released equal amounts of sTNF-α and sFasL during aging in vitro, and the TNF-α-knockdown CCs secreted less sFasL than the control CCs did. Treatment of DOs in vitro with sTNF-α significantly accelerated their aging. The aging-promoting effect of sTNF-α was significantly reduced in TNF-α-knocked-down CCs and in CCs from the TNF-α-knockout mice. It is concluded that mouse CCs accelerate oocyte aging by secreting sTNF-α as well as sFasL. Impact Journals 2018-07-26 /pmc/articles/PMC6075436/ /pubmed/30048240 http://dx.doi.org/10.18632/aging.101507 Text en Copyright © 2018 Kong et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Kong, Qiao-Qiao
Wang, Jia
Xiao, Bin
Lin, Fei-Hu
Zhu, Jiang
Sun, Guang-Yi
Luo, Ming-Jiu
Tan, Jing-He
Cumulus cell-released tumor necrosis factor (TNF)-α promotes post-ovulatory aging of mouse oocytes
title Cumulus cell-released tumor necrosis factor (TNF)-α promotes post-ovulatory aging of mouse oocytes
title_full Cumulus cell-released tumor necrosis factor (TNF)-α promotes post-ovulatory aging of mouse oocytes
title_fullStr Cumulus cell-released tumor necrosis factor (TNF)-α promotes post-ovulatory aging of mouse oocytes
title_full_unstemmed Cumulus cell-released tumor necrosis factor (TNF)-α promotes post-ovulatory aging of mouse oocytes
title_short Cumulus cell-released tumor necrosis factor (TNF)-α promotes post-ovulatory aging of mouse oocytes
title_sort cumulus cell-released tumor necrosis factor (tnf)-α promotes post-ovulatory aging of mouse oocytes
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6075436/
https://www.ncbi.nlm.nih.gov/pubmed/30048240
http://dx.doi.org/10.18632/aging.101507
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