Cargando…
The use of in-strip digestion for fast proteomic analysis on tear fluid from dry eye patients
Tear is an accessible fluid for exploring biomarkers of dry eye disease. This study describes a fast proteomic method by LC-Q-orbitrap-MS analysis with in-strip digestion and investigates the tear proteome of dry eye patients. Schirmer’s strips were used for collection of tear fluid from patients. T...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6075744/ https://www.ncbi.nlm.nih.gov/pubmed/30074997 http://dx.doi.org/10.1371/journal.pone.0200702 |
_version_ | 1783344606631428096 |
---|---|
author | Huang, Zhu Du, Chi-Xin Pan, Xiao-Dong |
author_facet | Huang, Zhu Du, Chi-Xin Pan, Xiao-Dong |
author_sort | Huang, Zhu |
collection | PubMed |
description | Tear is an accessible fluid for exploring biomarkers of dry eye disease. This study describes a fast proteomic method by LC-Q-orbitrap-MS analysis with in-strip digestion and investigates the tear proteome of dry eye patients. Schirmer’s strips were used for collection of tear fluid from patients. These strips were cut into pieces and directly digested with trypsin before mass spectrometry analysis. The data showed that more than 50 proteins were found in tear fluid from dry eye patients. Gene Ontology (GO) annotation showed that most of proteins were transfer/carrier proteins, hydrolyses, enzyme modulators and signaling molecules. Targeted proteomics strategy revealed that 18 proteins were differentially expressed in dry eye patients. Furthermore, it was showed that the common post-translational modification in tear proteins is deamidation of Asn. |
format | Online Article Text |
id | pubmed-6075744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-60757442018-08-16 The use of in-strip digestion for fast proteomic analysis on tear fluid from dry eye patients Huang, Zhu Du, Chi-Xin Pan, Xiao-Dong PLoS One Research Article Tear is an accessible fluid for exploring biomarkers of dry eye disease. This study describes a fast proteomic method by LC-Q-orbitrap-MS analysis with in-strip digestion and investigates the tear proteome of dry eye patients. Schirmer’s strips were used for collection of tear fluid from patients. These strips were cut into pieces and directly digested with trypsin before mass spectrometry analysis. The data showed that more than 50 proteins were found in tear fluid from dry eye patients. Gene Ontology (GO) annotation showed that most of proteins were transfer/carrier proteins, hydrolyses, enzyme modulators and signaling molecules. Targeted proteomics strategy revealed that 18 proteins were differentially expressed in dry eye patients. Furthermore, it was showed that the common post-translational modification in tear proteins is deamidation of Asn. Public Library of Science 2018-08-03 /pmc/articles/PMC6075744/ /pubmed/30074997 http://dx.doi.org/10.1371/journal.pone.0200702 Text en © 2018 Huang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Huang, Zhu Du, Chi-Xin Pan, Xiao-Dong The use of in-strip digestion for fast proteomic analysis on tear fluid from dry eye patients |
title | The use of in-strip digestion for fast proteomic analysis on tear fluid from dry eye patients |
title_full | The use of in-strip digestion for fast proteomic analysis on tear fluid from dry eye patients |
title_fullStr | The use of in-strip digestion for fast proteomic analysis on tear fluid from dry eye patients |
title_full_unstemmed | The use of in-strip digestion for fast proteomic analysis on tear fluid from dry eye patients |
title_short | The use of in-strip digestion for fast proteomic analysis on tear fluid from dry eye patients |
title_sort | use of in-strip digestion for fast proteomic analysis on tear fluid from dry eye patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6075744/ https://www.ncbi.nlm.nih.gov/pubmed/30074997 http://dx.doi.org/10.1371/journal.pone.0200702 |
work_keys_str_mv | AT huangzhu theuseofinstripdigestionforfastproteomicanalysisontearfluidfromdryeyepatients AT duchixin theuseofinstripdigestionforfastproteomicanalysisontearfluidfromdryeyepatients AT panxiaodong theuseofinstripdigestionforfastproteomicanalysisontearfluidfromdryeyepatients AT huangzhu useofinstripdigestionforfastproteomicanalysisontearfluidfromdryeyepatients AT duchixin useofinstripdigestionforfastproteomicanalysisontearfluidfromdryeyepatients AT panxiaodong useofinstripdigestionforfastproteomicanalysisontearfluidfromdryeyepatients |