Reversible RNA Modification N(1)-methyladenosine (m(1)A) in mRNA and tRNA

More than 100 modifications have been found in RNA. Analogous to epigenetic DNA methylation, epitranscriptomic modifications can be written, read, and erased by a complex network of proteins. Apart from N(6)-methyladenosine (m(6)A), N(1)-methyladenosine (m(1)A) has been found as a reversible modific...

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Detalles Bibliográficos
Autores principales: Zhang, Chi, Jia, Guifang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6076376/
https://www.ncbi.nlm.nih.gov/pubmed/29908293
http://dx.doi.org/10.1016/j.gpb.2018.03.003
Descripción
Sumario:More than 100 modifications have been found in RNA. Analogous to epigenetic DNA methylation, epitranscriptomic modifications can be written, read, and erased by a complex network of proteins. Apart from N(6)-methyladenosine (m(6)A), N(1)-methyladenosine (m(1)A) has been found as a reversible modification in tRNA and mRNA. m(1)A occurs at positions 9, 14, and 58 of tRNA, with m(1)A58 being critical for tRNA stability. Other than the hundreds of m(1)A sites in mRNA and long non-coding RNA transcripts, transcriptome-wide mapping of m(1)A also identifies >20 m(1)A sites in mitochondrial genes. m(1)A in the coding region of mitochondrial transcripts can inhibit the translation of the corresponding proteins. In this review, we summarize the current understanding of m(1)A in mRNA and tRNA, covering high-throughput sequencing methods developed for m(1)A methylome, m(1)A-related enzymes (writers and erasers), as well as its functions in mRNA and tRNA.

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