Reversible RNA Modification N(1)-methyladenosine (m(1)A) in mRNA and tRNA

More than 100 modifications have been found in RNA. Analogous to epigenetic DNA methylation, epitranscriptomic modifications can be written, read, and erased by a complex network of proteins. Apart from N(6)-methyladenosine (m(6)A), N(1)-methyladenosine (m(1)A) has been found as a reversible modific...

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Detalles Bibliográficos
Autores principales: Zhang, Chi, Jia, Guifang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6076376/
https://www.ncbi.nlm.nih.gov/pubmed/29908293
http://dx.doi.org/10.1016/j.gpb.2018.03.003
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author Zhang, Chi
Jia, Guifang
author_facet Zhang, Chi
Jia, Guifang
author_sort Zhang, Chi
collection PubMed
description More than 100 modifications have been found in RNA. Analogous to epigenetic DNA methylation, epitranscriptomic modifications can be written, read, and erased by a complex network of proteins. Apart from N(6)-methyladenosine (m(6)A), N(1)-methyladenosine (m(1)A) has been found as a reversible modification in tRNA and mRNA. m(1)A occurs at positions 9, 14, and 58 of tRNA, with m(1)A58 being critical for tRNA stability. Other than the hundreds of m(1)A sites in mRNA and long non-coding RNA transcripts, transcriptome-wide mapping of m(1)A also identifies >20 m(1)A sites in mitochondrial genes. m(1)A in the coding region of mitochondrial transcripts can inhibit the translation of the corresponding proteins. In this review, we summarize the current understanding of m(1)A in mRNA and tRNA, covering high-throughput sequencing methods developed for m(1)A methylome, m(1)A-related enzymes (writers and erasers), as well as its functions in mRNA and tRNA.
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spelling pubmed-60763762018-08-09 Reversible RNA Modification N(1)-methyladenosine (m(1)A) in mRNA and tRNA Zhang, Chi Jia, Guifang Genomics Proteomics Bioinformatics Review More than 100 modifications have been found in RNA. Analogous to epigenetic DNA methylation, epitranscriptomic modifications can be written, read, and erased by a complex network of proteins. Apart from N(6)-methyladenosine (m(6)A), N(1)-methyladenosine (m(1)A) has been found as a reversible modification in tRNA and mRNA. m(1)A occurs at positions 9, 14, and 58 of tRNA, with m(1)A58 being critical for tRNA stability. Other than the hundreds of m(1)A sites in mRNA and long non-coding RNA transcripts, transcriptome-wide mapping of m(1)A also identifies >20 m(1)A sites in mitochondrial genes. m(1)A in the coding region of mitochondrial transcripts can inhibit the translation of the corresponding proteins. In this review, we summarize the current understanding of m(1)A in mRNA and tRNA, covering high-throughput sequencing methods developed for m(1)A methylome, m(1)A-related enzymes (writers and erasers), as well as its functions in mRNA and tRNA. Elsevier 2018-06 2018-06-14 /pmc/articles/PMC6076376/ /pubmed/29908293 http://dx.doi.org/10.1016/j.gpb.2018.03.003 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Review
Zhang, Chi
Jia, Guifang
Reversible RNA Modification N(1)-methyladenosine (m(1)A) in mRNA and tRNA
title Reversible RNA Modification N(1)-methyladenosine (m(1)A) in mRNA and tRNA
title_full Reversible RNA Modification N(1)-methyladenosine (m(1)A) in mRNA and tRNA
title_fullStr Reversible RNA Modification N(1)-methyladenosine (m(1)A) in mRNA and tRNA
title_full_unstemmed Reversible RNA Modification N(1)-methyladenosine (m(1)A) in mRNA and tRNA
title_short Reversible RNA Modification N(1)-methyladenosine (m(1)A) in mRNA and tRNA
title_sort reversible rna modification n(1)-methyladenosine (m(1)a) in mrna and trna
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6076376/
https://www.ncbi.nlm.nih.gov/pubmed/29908293
http://dx.doi.org/10.1016/j.gpb.2018.03.003
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