Emulsion Cross-Linking Technique for Human Fibroblast Encapsulation

Microencapsulation with biodegradable polymers has potential application in drug and cell delivery systems and is currently used in probiotic delivery. In the present study, microcapsules of human fibroblast cells (CRL2522) were prepared by emulsion cross-linking technique. Tween 80 surfactant at a...

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Detalles Bibliográficos
Autores principales: Chaemsawang, Watcharaphong, Prasongchean, Weerapong, Papadopoulos, Konstantinos I., Sukrong, Suchada, Kao, W. John, Wattanaarsakit, Phanphen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6076944/
https://www.ncbi.nlm.nih.gov/pubmed/30105055
http://dx.doi.org/10.1155/2018/9317878
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author Chaemsawang, Watcharaphong
Prasongchean, Weerapong
Papadopoulos, Konstantinos I.
Sukrong, Suchada
Kao, W. John
Wattanaarsakit, Phanphen
author_facet Chaemsawang, Watcharaphong
Prasongchean, Weerapong
Papadopoulos, Konstantinos I.
Sukrong, Suchada
Kao, W. John
Wattanaarsakit, Phanphen
author_sort Chaemsawang, Watcharaphong
collection PubMed
description Microencapsulation with biodegradable polymers has potential application in drug and cell delivery systems and is currently used in probiotic delivery. In the present study, microcapsules of human fibroblast cells (CRL2522) were prepared by emulsion cross-linking technique. Tween 80 surfactant at a 2% concentration through phase inversion resulted in the most efficient and stable size, morphology, and the cells survival at least 50% on day 14. Emulsion cross-linking microcapsule preparation resulted in smaller and possibly more diverse particles that can be developed clinically to deliver encapsulated mammalian cells for future disease treatments.
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spelling pubmed-60769442018-08-13 Emulsion Cross-Linking Technique for Human Fibroblast Encapsulation Chaemsawang, Watcharaphong Prasongchean, Weerapong Papadopoulos, Konstantinos I. Sukrong, Suchada Kao, W. John Wattanaarsakit, Phanphen Int J Biomater Research Article Microencapsulation with biodegradable polymers has potential application in drug and cell delivery systems and is currently used in probiotic delivery. In the present study, microcapsules of human fibroblast cells (CRL2522) were prepared by emulsion cross-linking technique. Tween 80 surfactant at a 2% concentration through phase inversion resulted in the most efficient and stable size, morphology, and the cells survival at least 50% on day 14. Emulsion cross-linking microcapsule preparation resulted in smaller and possibly more diverse particles that can be developed clinically to deliver encapsulated mammalian cells for future disease treatments. Hindawi 2018-07-11 /pmc/articles/PMC6076944/ /pubmed/30105055 http://dx.doi.org/10.1155/2018/9317878 Text en Copyright © 2018 Watcharaphong Chaemsawang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chaemsawang, Watcharaphong
Prasongchean, Weerapong
Papadopoulos, Konstantinos I.
Sukrong, Suchada
Kao, W. John
Wattanaarsakit, Phanphen
Emulsion Cross-Linking Technique for Human Fibroblast Encapsulation
title Emulsion Cross-Linking Technique for Human Fibroblast Encapsulation
title_full Emulsion Cross-Linking Technique for Human Fibroblast Encapsulation
title_fullStr Emulsion Cross-Linking Technique for Human Fibroblast Encapsulation
title_full_unstemmed Emulsion Cross-Linking Technique for Human Fibroblast Encapsulation
title_short Emulsion Cross-Linking Technique for Human Fibroblast Encapsulation
title_sort emulsion cross-linking technique for human fibroblast encapsulation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6076944/
https://www.ncbi.nlm.nih.gov/pubmed/30105055
http://dx.doi.org/10.1155/2018/9317878
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AT kaowjohn emulsioncrosslinkingtechniqueforhumanfibroblastencapsulation
AT wattanaarsakitphanphen emulsioncrosslinkingtechniqueforhumanfibroblastencapsulation

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