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Exercise and β-alanine supplementation on carnosine-acrolein adduct in skeletal muscle

Previous studies have demonstrated that exercise results in reactive aldehyde production and that β-alanine supplementation increases carnosine content in skeletal muscle. However, little is known about the influence exercise and β-alanine supplementation have on the formation of carnosine-aldehydes...

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Autores principales: Carvalho, Victor H., Oliveira, Ana H.S., de Oliveira, Luana F., da Silva, Rafael P., Di Mascio, Paolo, Gualano, Bruno, Artioli, Guilherme G., Medeiros, Marisa H.G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6077140/
https://www.ncbi.nlm.nih.gov/pubmed/30053728
http://dx.doi.org/10.1016/j.redox.2018.07.009
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author Carvalho, Victor H.
Oliveira, Ana H.S.
de Oliveira, Luana F.
da Silva, Rafael P.
Di Mascio, Paolo
Gualano, Bruno
Artioli, Guilherme G.
Medeiros, Marisa H.G.
author_facet Carvalho, Victor H.
Oliveira, Ana H.S.
de Oliveira, Luana F.
da Silva, Rafael P.
Di Mascio, Paolo
Gualano, Bruno
Artioli, Guilherme G.
Medeiros, Marisa H.G.
author_sort Carvalho, Victor H.
collection PubMed
description Previous studies have demonstrated that exercise results in reactive aldehyde production and that β-alanine supplementation increases carnosine content in skeletal muscle. However, little is known about the influence exercise and β-alanine supplementation have on the formation of carnosine-aldehydes. The goal of the present study was to monitor the formation of carnosine-aldehyde adducts, following high-intensity intermittent exercise, before and after β-alanine supplementation. Vastus lateralis biopsy samples were taken from 14 cyclists, before and after a 28 day β-alanine supplementation, following 4 bouts of a 30 s all-out cycling test, and carnosine and CAR-aldehyde adducts [carnosine-acrolein, CAR-ACR (m/z 303), carnosine-4-hydroxy-2-hexenal, CAR-HHE (m/z 341) and carnosine-4-hydroxy-2-nonenal, CAR-HNE (m/z 383)] were quantified by HPLC-MS/MS. β-alanine supplementation increased muscle carnosine content by ~50% (p = 0.0001 vs. Pre-Supplementation). Interestingly, there was a significant increase in post-exercise CAR-ACR content following β-alanine supplementation (p < 0.001 vs. post-exercise before supplementation), whereas neither exercise alone nor supplementation alone increased CAR-ACR formation. These results suggest that carnosine functions as an acrolein-scavenger in skeletal muscle. Such a role would be relevant to the detoxification of this aldehyde formed during exercise, and appears to be enhanced by β-alanine supplementation. These novel findings not only have the potential of directly benefiting athletes who engage in intensive training regimens, but will also allow researchers to explore the role of muscle carnosine in detoxifying reactive aldehydes in diseases characterized by abnormal oxidative stress.
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spelling pubmed-60771402018-08-09 Exercise and β-alanine supplementation on carnosine-acrolein adduct in skeletal muscle Carvalho, Victor H. Oliveira, Ana H.S. de Oliveira, Luana F. da Silva, Rafael P. Di Mascio, Paolo Gualano, Bruno Artioli, Guilherme G. Medeiros, Marisa H.G. Redox Biol Short Communication Previous studies have demonstrated that exercise results in reactive aldehyde production and that β-alanine supplementation increases carnosine content in skeletal muscle. However, little is known about the influence exercise and β-alanine supplementation have on the formation of carnosine-aldehydes. The goal of the present study was to monitor the formation of carnosine-aldehyde adducts, following high-intensity intermittent exercise, before and after β-alanine supplementation. Vastus lateralis biopsy samples were taken from 14 cyclists, before and after a 28 day β-alanine supplementation, following 4 bouts of a 30 s all-out cycling test, and carnosine and CAR-aldehyde adducts [carnosine-acrolein, CAR-ACR (m/z 303), carnosine-4-hydroxy-2-hexenal, CAR-HHE (m/z 341) and carnosine-4-hydroxy-2-nonenal, CAR-HNE (m/z 383)] were quantified by HPLC-MS/MS. β-alanine supplementation increased muscle carnosine content by ~50% (p = 0.0001 vs. Pre-Supplementation). Interestingly, there was a significant increase in post-exercise CAR-ACR content following β-alanine supplementation (p < 0.001 vs. post-exercise before supplementation), whereas neither exercise alone nor supplementation alone increased CAR-ACR formation. These results suggest that carnosine functions as an acrolein-scavenger in skeletal muscle. Such a role would be relevant to the detoxification of this aldehyde formed during exercise, and appears to be enhanced by β-alanine supplementation. These novel findings not only have the potential of directly benefiting athletes who engage in intensive training regimens, but will also allow researchers to explore the role of muscle carnosine in detoxifying reactive aldehydes in diseases characterized by abnormal oxidative stress. Elsevier 2018-07-18 /pmc/articles/PMC6077140/ /pubmed/30053728 http://dx.doi.org/10.1016/j.redox.2018.07.009 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Short Communication
Carvalho, Victor H.
Oliveira, Ana H.S.
de Oliveira, Luana F.
da Silva, Rafael P.
Di Mascio, Paolo
Gualano, Bruno
Artioli, Guilherme G.
Medeiros, Marisa H.G.
Exercise and β-alanine supplementation on carnosine-acrolein adduct in skeletal muscle
title Exercise and β-alanine supplementation on carnosine-acrolein adduct in skeletal muscle
title_full Exercise and β-alanine supplementation on carnosine-acrolein adduct in skeletal muscle
title_fullStr Exercise and β-alanine supplementation on carnosine-acrolein adduct in skeletal muscle
title_full_unstemmed Exercise and β-alanine supplementation on carnosine-acrolein adduct in skeletal muscle
title_short Exercise and β-alanine supplementation on carnosine-acrolein adduct in skeletal muscle
title_sort exercise and β-alanine supplementation on carnosine-acrolein adduct in skeletal muscle
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6077140/
https://www.ncbi.nlm.nih.gov/pubmed/30053728
http://dx.doi.org/10.1016/j.redox.2018.07.009
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