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A Long-Term Study of a Lipid-Buprenorphine Implant in Rats

Animal models to study opiates are of growing interest. We have examined the short-term safety of buprenorphine implants in Fischer F344/NTac rats treated with excess doses of a cholesterol-triglyceride suspension of buprenorphine. A single injection of 0.65 mg/kg afforded clinically significant blo...

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Detalles Bibliográficos
Autores principales: Guarnieri, Michael, Brayton, Cory, Tyler, Betty M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6077592/
https://www.ncbi.nlm.nih.gov/pubmed/30112418
http://dx.doi.org/10.1155/2018/2616152
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author Guarnieri, Michael
Brayton, Cory
Tyler, Betty M.
author_facet Guarnieri, Michael
Brayton, Cory
Tyler, Betty M.
author_sort Guarnieri, Michael
collection PubMed
description Animal models to study opiates are of growing interest. We have examined the short-term safety of buprenorphine implants in Fischer F344/NTac rats treated with excess doses of a cholesterol-triglyceride suspension of buprenorphine. A single injection of 0.65 mg/kg afforded clinically significant blood levels of analgesia for 3 days. Chemistry, hematology, coagulation, and urinalysis values with 2- to 10-fold excess doses of the drug-lipid suspension were within normal limits. Histopathology findings were unremarkable. The skin and underlying tissue surrounding the drug injection were unremarkable. Here we report the results of a long-term follow-up study of female rats injected with 0.65 and 1.3 mg/kg. The 14-month evaluation showed no abnormal findings that could be attributed to the drug or lipid suspension. These results confirm the safety of cholesterol-triglyceride carrier systems for subcutaneous drug delivery in laboratory animals and suggest that this model may be used to study long-term effects of opiate therapy.
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spelling pubmed-60775922018-08-15 A Long-Term Study of a Lipid-Buprenorphine Implant in Rats Guarnieri, Michael Brayton, Cory Tyler, Betty M. J Vet Med Research Article Animal models to study opiates are of growing interest. We have examined the short-term safety of buprenorphine implants in Fischer F344/NTac rats treated with excess doses of a cholesterol-triglyceride suspension of buprenorphine. A single injection of 0.65 mg/kg afforded clinically significant blood levels of analgesia for 3 days. Chemistry, hematology, coagulation, and urinalysis values with 2- to 10-fold excess doses of the drug-lipid suspension were within normal limits. Histopathology findings were unremarkable. The skin and underlying tissue surrounding the drug injection were unremarkable. Here we report the results of a long-term follow-up study of female rats injected with 0.65 and 1.3 mg/kg. The 14-month evaluation showed no abnormal findings that could be attributed to the drug or lipid suspension. These results confirm the safety of cholesterol-triglyceride carrier systems for subcutaneous drug delivery in laboratory animals and suggest that this model may be used to study long-term effects of opiate therapy. Hindawi 2018-07-09 /pmc/articles/PMC6077592/ /pubmed/30112418 http://dx.doi.org/10.1155/2018/2616152 Text en Copyright © 2018 Michael Guarnieri et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Guarnieri, Michael
Brayton, Cory
Tyler, Betty M.
A Long-Term Study of a Lipid-Buprenorphine Implant in Rats
title A Long-Term Study of a Lipid-Buprenorphine Implant in Rats
title_full A Long-Term Study of a Lipid-Buprenorphine Implant in Rats
title_fullStr A Long-Term Study of a Lipid-Buprenorphine Implant in Rats
title_full_unstemmed A Long-Term Study of a Lipid-Buprenorphine Implant in Rats
title_short A Long-Term Study of a Lipid-Buprenorphine Implant in Rats
title_sort long-term study of a lipid-buprenorphine implant in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6077592/
https://www.ncbi.nlm.nih.gov/pubmed/30112418
http://dx.doi.org/10.1155/2018/2616152
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