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A Long-Term Study of a Lipid-Buprenorphine Implant in Rats
Animal models to study opiates are of growing interest. We have examined the short-term safety of buprenorphine implants in Fischer F344/NTac rats treated with excess doses of a cholesterol-triglyceride suspension of buprenorphine. A single injection of 0.65 mg/kg afforded clinically significant blo...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6077592/ https://www.ncbi.nlm.nih.gov/pubmed/30112418 http://dx.doi.org/10.1155/2018/2616152 |
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author | Guarnieri, Michael Brayton, Cory Tyler, Betty M. |
author_facet | Guarnieri, Michael Brayton, Cory Tyler, Betty M. |
author_sort | Guarnieri, Michael |
collection | PubMed |
description | Animal models to study opiates are of growing interest. We have examined the short-term safety of buprenorphine implants in Fischer F344/NTac rats treated with excess doses of a cholesterol-triglyceride suspension of buprenorphine. A single injection of 0.65 mg/kg afforded clinically significant blood levels of analgesia for 3 days. Chemistry, hematology, coagulation, and urinalysis values with 2- to 10-fold excess doses of the drug-lipid suspension were within normal limits. Histopathology findings were unremarkable. The skin and underlying tissue surrounding the drug injection were unremarkable. Here we report the results of a long-term follow-up study of female rats injected with 0.65 and 1.3 mg/kg. The 14-month evaluation showed no abnormal findings that could be attributed to the drug or lipid suspension. These results confirm the safety of cholesterol-triglyceride carrier systems for subcutaneous drug delivery in laboratory animals and suggest that this model may be used to study long-term effects of opiate therapy. |
format | Online Article Text |
id | pubmed-6077592 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-60775922018-08-15 A Long-Term Study of a Lipid-Buprenorphine Implant in Rats Guarnieri, Michael Brayton, Cory Tyler, Betty M. J Vet Med Research Article Animal models to study opiates are of growing interest. We have examined the short-term safety of buprenorphine implants in Fischer F344/NTac rats treated with excess doses of a cholesterol-triglyceride suspension of buprenorphine. A single injection of 0.65 mg/kg afforded clinically significant blood levels of analgesia for 3 days. Chemistry, hematology, coagulation, and urinalysis values with 2- to 10-fold excess doses of the drug-lipid suspension were within normal limits. Histopathology findings were unremarkable. The skin and underlying tissue surrounding the drug injection were unremarkable. Here we report the results of a long-term follow-up study of female rats injected with 0.65 and 1.3 mg/kg. The 14-month evaluation showed no abnormal findings that could be attributed to the drug or lipid suspension. These results confirm the safety of cholesterol-triglyceride carrier systems for subcutaneous drug delivery in laboratory animals and suggest that this model may be used to study long-term effects of opiate therapy. Hindawi 2018-07-09 /pmc/articles/PMC6077592/ /pubmed/30112418 http://dx.doi.org/10.1155/2018/2616152 Text en Copyright © 2018 Michael Guarnieri et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Guarnieri, Michael Brayton, Cory Tyler, Betty M. A Long-Term Study of a Lipid-Buprenorphine Implant in Rats |
title | A Long-Term Study of a Lipid-Buprenorphine Implant in Rats |
title_full | A Long-Term Study of a Lipid-Buprenorphine Implant in Rats |
title_fullStr | A Long-Term Study of a Lipid-Buprenorphine Implant in Rats |
title_full_unstemmed | A Long-Term Study of a Lipid-Buprenorphine Implant in Rats |
title_short | A Long-Term Study of a Lipid-Buprenorphine Implant in Rats |
title_sort | long-term study of a lipid-buprenorphine implant in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6077592/ https://www.ncbi.nlm.nih.gov/pubmed/30112418 http://dx.doi.org/10.1155/2018/2616152 |
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