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“CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica
Amebiasis remains a major health problem in Mexico. Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6077601/ https://www.ncbi.nlm.nih.gov/pubmed/30112194 http://dx.doi.org/10.1155/2018/2796516 |
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author | Gonzalez-Salazar, F. Meester, I. Guzmán De La Garza, F. J. De La Garza-Salinas, L. H. Sampayo-Reyes, A. Garza-Gonzalez, J. N. Monsivais-Diaz, O. Barba-Dávila, B. A. Hernández-García, M. E. Vargas-Villarreal, J. |
author_facet | Gonzalez-Salazar, F. Meester, I. Guzmán De La Garza, F. J. De La Garza-Salinas, L. H. Sampayo-Reyes, A. Garza-Gonzalez, J. N. Monsivais-Diaz, O. Barba-Dávila, B. A. Hernández-García, M. E. Vargas-Villarreal, J. |
author_sort | Gonzalez-Salazar, F. |
collection | PubMed |
description | Amebiasis remains a major health problem in Mexico. Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. The novel CLUPS medium is an improved version of the PEHPS medium, previously designed in our laboratory. The main difference is the substitution of raw beef liver in PEHPS by raw beef lung in the CLUPS medium. To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS), E. histolytica trophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters. E. histolytica trophozoites grew significantly better in CLUPS medium than in TYI-S-33. Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, trophozoites grown in any of the three tested media had similar potential to induce liver abscesses. |
format | Online Article Text |
id | pubmed-6077601 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-60776012018-08-15 “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica Gonzalez-Salazar, F. Meester, I. Guzmán De La Garza, F. J. De La Garza-Salinas, L. H. Sampayo-Reyes, A. Garza-Gonzalez, J. N. Monsivais-Diaz, O. Barba-Dávila, B. A. Hernández-García, M. E. Vargas-Villarreal, J. J Parasitol Res Research Article Amebiasis remains a major health problem in Mexico. Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. The novel CLUPS medium is an improved version of the PEHPS medium, previously designed in our laboratory. The main difference is the substitution of raw beef liver in PEHPS by raw beef lung in the CLUPS medium. To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS), E. histolytica trophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters. E. histolytica trophozoites grew significantly better in CLUPS medium than in TYI-S-33. Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, trophozoites grown in any of the three tested media had similar potential to induce liver abscesses. Hindawi 2018-07-11 /pmc/articles/PMC6077601/ /pubmed/30112194 http://dx.doi.org/10.1155/2018/2796516 Text en Copyright © 2018 F. Gonzalez-Salazar et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gonzalez-Salazar, F. Meester, I. Guzmán De La Garza, F. J. De La Garza-Salinas, L. H. Sampayo-Reyes, A. Garza-Gonzalez, J. N. Monsivais-Diaz, O. Barba-Dávila, B. A. Hernández-García, M. E. Vargas-Villarreal, J. “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica |
title | “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica |
title_full | “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica |
title_fullStr | “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica |
title_full_unstemmed | “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica |
title_short | “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica |
title_sort | “clups”: a new culture medium for the axenic growth of entamoeba histolytica |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6077601/ https://www.ncbi.nlm.nih.gov/pubmed/30112194 http://dx.doi.org/10.1155/2018/2796516 |
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