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Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes
Circadian rhythms are cell‐autonomous biological oscillations with a period of about 24 h. Current models propose that transcriptional feedback loops are the primary mechanism for the generation of circadian oscillations. Within this framework, Drosophila S2 cells are regarded as “non‐rhythmic” cell...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078164/ https://www.ncbi.nlm.nih.gov/pubmed/30072421 http://dx.doi.org/10.15252/msb.20188376 |
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author | Rey, Guillaume Milev, Nikolay B Valekunja, Utham K Ch, Ratnasekhar Ray, Sandipan Silva Dos Santos, Mariana Nagy, Andras D Antrobus, Robin MacRae, James I Reddy, Akhilesh B |
author_facet | Rey, Guillaume Milev, Nikolay B Valekunja, Utham K Ch, Ratnasekhar Ray, Sandipan Silva Dos Santos, Mariana Nagy, Andras D Antrobus, Robin MacRae, James I Reddy, Akhilesh B |
author_sort | Rey, Guillaume |
collection | PubMed |
description | Circadian rhythms are cell‐autonomous biological oscillations with a period of about 24 h. Current models propose that transcriptional feedback loops are the primary mechanism for the generation of circadian oscillations. Within this framework, Drosophila S2 cells are regarded as “non‐rhythmic” cells, as they do not express several canonical circadian components. Using an unbiased multi‐omics approach, we made the surprising discovery that Drosophila S2 cells do in fact display widespread daily rhythms. Transcriptomics and proteomics analyses revealed that hundreds of genes and their products, and in particular metabolic enzymes, are rhythmically expressed in a 24‐h cycle. Metabolomics analyses extended these findings and demonstrate that central carbon metabolism and amino acid metabolism are core metabolic pathways driven by protein rhythms. We thus demonstrate that 24‐h metabolic oscillations, coupled to gene and protein cycles, take place in nucleated cells without the contribution of any known circadian regulators. These results therefore suggest a reconsideration of existing models of the clockwork in Drosophila and other eukaryotic systems. |
format | Online Article Text |
id | pubmed-6078164 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60781642018-08-09 Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes Rey, Guillaume Milev, Nikolay B Valekunja, Utham K Ch, Ratnasekhar Ray, Sandipan Silva Dos Santos, Mariana Nagy, Andras D Antrobus, Robin MacRae, James I Reddy, Akhilesh B Mol Syst Biol Articles Circadian rhythms are cell‐autonomous biological oscillations with a period of about 24 h. Current models propose that transcriptional feedback loops are the primary mechanism for the generation of circadian oscillations. Within this framework, Drosophila S2 cells are regarded as “non‐rhythmic” cells, as they do not express several canonical circadian components. Using an unbiased multi‐omics approach, we made the surprising discovery that Drosophila S2 cells do in fact display widespread daily rhythms. Transcriptomics and proteomics analyses revealed that hundreds of genes and their products, and in particular metabolic enzymes, are rhythmically expressed in a 24‐h cycle. Metabolomics analyses extended these findings and demonstrate that central carbon metabolism and amino acid metabolism are core metabolic pathways driven by protein rhythms. We thus demonstrate that 24‐h metabolic oscillations, coupled to gene and protein cycles, take place in nucleated cells without the contribution of any known circadian regulators. These results therefore suggest a reconsideration of existing models of the clockwork in Drosophila and other eukaryotic systems. John Wiley and Sons Inc. 2018-08-06 /pmc/articles/PMC6078164/ /pubmed/30072421 http://dx.doi.org/10.15252/msb.20188376 Text en © 2018 The Authors. Published under the terms of the CC BY 4.0 license This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Rey, Guillaume Milev, Nikolay B Valekunja, Utham K Ch, Ratnasekhar Ray, Sandipan Silva Dos Santos, Mariana Nagy, Andras D Antrobus, Robin MacRae, James I Reddy, Akhilesh B Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes |
title | Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes |
title_full | Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes |
title_fullStr | Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes |
title_full_unstemmed | Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes |
title_short | Metabolic oscillations on the circadian time scale in Drosophila cells lacking clock genes |
title_sort | metabolic oscillations on the circadian time scale in drosophila cells lacking clock genes |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078164/ https://www.ncbi.nlm.nih.gov/pubmed/30072421 http://dx.doi.org/10.15252/msb.20188376 |
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