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In depth investigation of the metabolism of Nectandra megapotamica chemotypes

Plants produce a wide range of secondary metabolites. Within a single species, chemotypes can be distinguished by the differences in the composition of the secondary metabolites. Herein, we evaluated Nectandra megapotamica (Spreng.) chemotypes and the balance of different classes of metabolites to v...

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Autores principales: Farias, Katyuce de S., Delatte, Thierry, Arruda, Rosani do C. de O., Alves, Flavio M., Silva, Denise B., Beekwilder, Jules, Carollo, Carlos A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078319/
https://www.ncbi.nlm.nih.gov/pubmed/30080887
http://dx.doi.org/10.1371/journal.pone.0201996
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author Farias, Katyuce de S.
Delatte, Thierry
Arruda, Rosani do C. de O.
Alves, Flavio M.
Silva, Denise B.
Beekwilder, Jules
Carollo, Carlos A.
author_facet Farias, Katyuce de S.
Delatte, Thierry
Arruda, Rosani do C. de O.
Alves, Flavio M.
Silva, Denise B.
Beekwilder, Jules
Carollo, Carlos A.
author_sort Farias, Katyuce de S.
collection PubMed
description Plants produce a wide range of secondary metabolites. Within a single species, chemotypes can be distinguished by the differences in the composition of the secondary metabolites. Herein, we evaluated Nectandra megapotamica (Spreng.) chemotypes and the balance of different classes of metabolites to verify how significant differences in plant metabolism are regarding chemotypes. We collected N. megapotamica leaves from eight adult plants in two Brazilian states. The essential oils and ethanol/water extracts were analyzed by GC-MS and LC-DAD-MS, respectively. Histochemical tests were performed, as well as chemical analyses of leaves from adaxial and abaxial foliar surfaces of N. megapotamica, and the stereochemistry of α-bisabolol was determined. Two different chemotypes, based on volatile compounds, were identified, distinguished by the presence of isospathulenol, α-bisabolol, β-bisabolene, and (E)-nerolidol for chemotype A, and bicyclogermacrene and elemicin for chemotype B. A stereochemical analysis of chemotype A extract revealed (+)-α-bisabolol enantiomer. Histochemical tests of chemotypes showed similar results and suggested the presence of essential oil in idioblasts stained with the dye NADI. The analyses of chemotype A leaves by GC-MS revealed similar compositions for abaxial and adaxial surfaces, such pattern was also observed for chemotype B. Medium and high polarity metabolites showed high chemical similarities between the chemotypes, highlighting the presence of proanthocyanidins and glycosylated flavonoids (O- and C-glycosides). Thus, N. megapotamica produced distinct volatile chemotypes with highly conserved medium to high polarity compounds. Such results suggest that phenolic derivatives have a basal physiological function, while genetic or environmental differences lead to differentiation in volatile profiles of N. megapotamica.
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spelling pubmed-60783192018-08-28 In depth investigation of the metabolism of Nectandra megapotamica chemotypes Farias, Katyuce de S. Delatte, Thierry Arruda, Rosani do C. de O. Alves, Flavio M. Silva, Denise B. Beekwilder, Jules Carollo, Carlos A. PLoS One Research Article Plants produce a wide range of secondary metabolites. Within a single species, chemotypes can be distinguished by the differences in the composition of the secondary metabolites. Herein, we evaluated Nectandra megapotamica (Spreng.) chemotypes and the balance of different classes of metabolites to verify how significant differences in plant metabolism are regarding chemotypes. We collected N. megapotamica leaves from eight adult plants in two Brazilian states. The essential oils and ethanol/water extracts were analyzed by GC-MS and LC-DAD-MS, respectively. Histochemical tests were performed, as well as chemical analyses of leaves from adaxial and abaxial foliar surfaces of N. megapotamica, and the stereochemistry of α-bisabolol was determined. Two different chemotypes, based on volatile compounds, were identified, distinguished by the presence of isospathulenol, α-bisabolol, β-bisabolene, and (E)-nerolidol for chemotype A, and bicyclogermacrene and elemicin for chemotype B. A stereochemical analysis of chemotype A extract revealed (+)-α-bisabolol enantiomer. Histochemical tests of chemotypes showed similar results and suggested the presence of essential oil in idioblasts stained with the dye NADI. The analyses of chemotype A leaves by GC-MS revealed similar compositions for abaxial and adaxial surfaces, such pattern was also observed for chemotype B. Medium and high polarity metabolites showed high chemical similarities between the chemotypes, highlighting the presence of proanthocyanidins and glycosylated flavonoids (O- and C-glycosides). Thus, N. megapotamica produced distinct volatile chemotypes with highly conserved medium to high polarity compounds. Such results suggest that phenolic derivatives have a basal physiological function, while genetic or environmental differences lead to differentiation in volatile profiles of N. megapotamica. Public Library of Science 2018-08-06 /pmc/articles/PMC6078319/ /pubmed/30080887 http://dx.doi.org/10.1371/journal.pone.0201996 Text en © 2018 Farias et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Farias, Katyuce de S.
Delatte, Thierry
Arruda, Rosani do C. de O.
Alves, Flavio M.
Silva, Denise B.
Beekwilder, Jules
Carollo, Carlos A.
In depth investigation of the metabolism of Nectandra megapotamica chemotypes
title In depth investigation of the metabolism of Nectandra megapotamica chemotypes
title_full In depth investigation of the metabolism of Nectandra megapotamica chemotypes
title_fullStr In depth investigation of the metabolism of Nectandra megapotamica chemotypes
title_full_unstemmed In depth investigation of the metabolism of Nectandra megapotamica chemotypes
title_short In depth investigation of the metabolism of Nectandra megapotamica chemotypes
title_sort in depth investigation of the metabolism of nectandra megapotamica chemotypes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078319/
https://www.ncbi.nlm.nih.gov/pubmed/30080887
http://dx.doi.org/10.1371/journal.pone.0201996
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