Extending chemical perturbations of the ubiquitin fitness landscape in a classroom setting reveals new constraints on sequence tolerance

Although the primary protein sequence of ubiquitin (Ub) is extremely stable over evolutionary time, it is highly tolerant to mutation during selection experiments performed in the laboratory. We have proposed that this discrepancy results from the difference between fitness under laboratory culture...

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Detalles Bibliográficos
Autores principales: Mavor, David, Barlow, Kyle A., Asarnow, Daniel, Birman, Yuliya, Britain, Derek, Chen, Weilin, Green, Evan M., Kenner, Lillian R., Mensa, Bruk, Morinishi, Leanna S., Nelson, Charlotte A., Poss, Erin M., Suresh, Pooja, Tian, Ruilin, Arhar, Taylor, Ary, Beatrice E., Bauer, David P., Bergman, Ian D., Brunetti, Rachel M., Chio, Cynthia M., Dai, Shizhong A., Dickinson, Miles S., Elledge, Susanna K., Helsell, Cole V. M., Hendel, Nathan L., Kang, Emily, Kern, Nadja, Khoroshkin, Matvei S., Kirkemo, Lisa L., Lewis, Greyson R., Lou, Kevin, Marin, Wesley M., Maxwell, Alison M., McTigue, Peter F., Myers-Turnbull, Douglas, Nagy, Tamas L., Natale, Andrew M., Oltion, Keely, Pourmal, Sergei, Reder, Gabriel K., Rettko, Nicholas J., Rohweder, Peter J., Schwarz, Daniel M. C, Tan, Sophia K., Thomas, Paul V., Tibble, Ryan W., Town, Jason P., Tsai, Mary K., Ugur, Fatima S., Wassarman, Douglas R., Wolff, Alexander M., Wu, Taia S., Bogdanoff, Derek, Li, Jennifer, Thorn, Kurt S., O'Conchúir, Shane, Swaney, Danielle L., Chow, Eric D., Madhani, Hiten D., Redding, Sy, Bolon, Daniel N., Kortemme, Tanja, DeRisi, Joseph L., Kampmann, Martin, Fraser, James S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078352/
https://www.ncbi.nlm.nih.gov/pubmed/30037883
http://dx.doi.org/10.1242/bio.036103

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