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Necroptosis Is a Mechanism of Death in Mouse Induced Hepatocyte-Like Cells Reprogrammed from Mouse Embryonic Fibroblasts

Liver transplantation is recommended for patients with liver failure, but liver donors are limited. This necessitates the development of artificial livers, and hepatocytes are necessary to develop such artificial livers. Although induced hepatocyte-like cells are used in artificial livers, the chara...

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Autores principales: Lee, Yun-Suk, Park, Kyung-Mee, Yu, Lina, Kwak, Ho-Hyun, Na, Hee-Jun, Kang, Kyung-Sun, Woo, Heung-Myong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078850/
https://www.ncbi.nlm.nih.gov/pubmed/29991669
http://dx.doi.org/10.14348/molcells.2018.2353
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author Lee, Yun-Suk
Park, Kyung-Mee
Yu, Lina
Kwak, Ho-Hyun
Na, Hee-Jun
Kang, Kyung-Sun
Woo, Heung-Myong
author_facet Lee, Yun-Suk
Park, Kyung-Mee
Yu, Lina
Kwak, Ho-Hyun
Na, Hee-Jun
Kang, Kyung-Sun
Woo, Heung-Myong
author_sort Lee, Yun-Suk
collection PubMed
description Liver transplantation is recommended for patients with liver failure, but liver donors are limited. This necessitates the development of artificial livers, and hepatocytes are necessary to develop such artificial livers. Although induced hepatocyte-like cells are used in artificial livers, the characteristics of mouse induced hepatocyte-like cells (miHeps) reprogrammed with embryonic fibroblasts have not yet been clarified. Therefore, this study investigated the mechanisms underlying the survival, function, and death of miHeps. miHeps showed decreased cell viability, increased cytotoxicity, decreased hepatic function, and albumin and urea secretion at passage 14. Addition of necrostatin-1 (NEC-1) to miHeps inhibited necrosome formation and reactive oxygen species generation and increased cell survival. However, NEC-1 did not affect the hepatic function of miHeps. These results provide a basis for development of artificial livers using hepatocytes.
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spelling pubmed-60788502018-08-23 Necroptosis Is a Mechanism of Death in Mouse Induced Hepatocyte-Like Cells Reprogrammed from Mouse Embryonic Fibroblasts Lee, Yun-Suk Park, Kyung-Mee Yu, Lina Kwak, Ho-Hyun Na, Hee-Jun Kang, Kyung-Sun Woo, Heung-Myong Mol Cells Article Liver transplantation is recommended for patients with liver failure, but liver donors are limited. This necessitates the development of artificial livers, and hepatocytes are necessary to develop such artificial livers. Although induced hepatocyte-like cells are used in artificial livers, the characteristics of mouse induced hepatocyte-like cells (miHeps) reprogrammed with embryonic fibroblasts have not yet been clarified. Therefore, this study investigated the mechanisms underlying the survival, function, and death of miHeps. miHeps showed decreased cell viability, increased cytotoxicity, decreased hepatic function, and albumin and urea secretion at passage 14. Addition of necrostatin-1 (NEC-1) to miHeps inhibited necrosome formation and reactive oxygen species generation and increased cell survival. However, NEC-1 did not affect the hepatic function of miHeps. These results provide a basis for development of artificial livers using hepatocytes. Korean Society for Molecular and Cellular Biology 2018-07-31 2018-07-10 /pmc/articles/PMC6078850/ /pubmed/29991669 http://dx.doi.org/10.14348/molcells.2018.2353 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
spellingShingle Article
Lee, Yun-Suk
Park, Kyung-Mee
Yu, Lina
Kwak, Ho-Hyun
Na, Hee-Jun
Kang, Kyung-Sun
Woo, Heung-Myong
Necroptosis Is a Mechanism of Death in Mouse Induced Hepatocyte-Like Cells Reprogrammed from Mouse Embryonic Fibroblasts
title Necroptosis Is a Mechanism of Death in Mouse Induced Hepatocyte-Like Cells Reprogrammed from Mouse Embryonic Fibroblasts
title_full Necroptosis Is a Mechanism of Death in Mouse Induced Hepatocyte-Like Cells Reprogrammed from Mouse Embryonic Fibroblasts
title_fullStr Necroptosis Is a Mechanism of Death in Mouse Induced Hepatocyte-Like Cells Reprogrammed from Mouse Embryonic Fibroblasts
title_full_unstemmed Necroptosis Is a Mechanism of Death in Mouse Induced Hepatocyte-Like Cells Reprogrammed from Mouse Embryonic Fibroblasts
title_short Necroptosis Is a Mechanism of Death in Mouse Induced Hepatocyte-Like Cells Reprogrammed from Mouse Embryonic Fibroblasts
title_sort necroptosis is a mechanism of death in mouse induced hepatocyte-like cells reprogrammed from mouse embryonic fibroblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078850/
https://www.ncbi.nlm.nih.gov/pubmed/29991669
http://dx.doi.org/10.14348/molcells.2018.2353
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