Directed evolution of CRISPR-Cas9 to increase its specificity

The use of CRISPR-Cas9 as a therapeutic reagent is hampered by its off-target effects. Although rationally designed S. pyogenes Cas9 (SpCas9) variants that display higher specificities than the wild-type SpCas9 protein are available, these attenuated Cas9 variants are often poorly efficient in human...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Jungjoon K., Jeong, Euihwan, Lee, Joonsun, Jung, Minhee, Shin, Eunji, Kim, Young-hoon, Lee, Kangin, Jung, Inyoung, Kim, Daesik, Kim, Seokjoong, Kim, Jin-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078992/
https://www.ncbi.nlm.nih.gov/pubmed/30082838
http://dx.doi.org/10.1038/s41467-018-05477-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078992/
https://www.ncbi.nlm.nih.gov/pubmed/30082838
http://dx.doi.org/10.1038/s41467-018-05477-x

Ejemplares similares