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Directed evolution of CRISPR-Cas9 to increase its specificity

The use of CRISPR-Cas9 as a therapeutic reagent is hampered by its off-target effects. Although rationally designed S. pyogenes Cas9 (SpCas9) variants that display higher specificities than the wild-type SpCas9 protein are available, these attenuated Cas9 variants are often poorly efficient in human...

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Detalles Bibliográficos
Autores principales:	Lee, Jungjoon K., Jeong, Euihwan, Lee, Joonsun, Jung, Minhee, Shin, Eunji, Kim, Young-hoon, Lee, Kangin, Jung, Inyoung, Kim, Daesik, Kim, Seokjoong, Kim, Jin-Soo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078992/ https://www.ncbi.nlm.nih.gov/pubmed/30082838 http://dx.doi.org/10.1038/s41467-018-05477-x

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