Cargando…

The RLR/NLR expression and pro-inflammatory activity of tissue mast cells are regulated by cathelicidin LL-37 and defensin hBD-2

Considering the significance of mast cells (MCs) in the course of various physiological and pathological processes, and the pivotal role of endogenous molecules, i.e., cathelicidins and defensins as multifunctional modulators, the study examines the constitutive and cathelicidin LL-37/defensin hBD-2...

Descripción completa

Detalles Bibliográficos
Autores principales: Agier, Justyna, Różalska, Sylwia, Wiktorska, Magdalena, Żelechowska, Paulina, Pastwińska, Joanna, Brzezińska-Błaszczyk, Ewa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079022/
https://www.ncbi.nlm.nih.gov/pubmed/30082780
http://dx.doi.org/10.1038/s41598-018-30289-w
_version_ 1783345190991298560
author Agier, Justyna
Różalska, Sylwia
Wiktorska, Magdalena
Żelechowska, Paulina
Pastwińska, Joanna
Brzezińska-Błaszczyk, Ewa
author_facet Agier, Justyna
Różalska, Sylwia
Wiktorska, Magdalena
Żelechowska, Paulina
Pastwińska, Joanna
Brzezińska-Błaszczyk, Ewa
author_sort Agier, Justyna
collection PubMed
description Considering the significance of mast cells (MCs) in the course of various physiological and pathological processes, and the pivotal role of endogenous molecules, i.e., cathelicidins and defensins as multifunctional modulators, the study examines the constitutive and cathelicidin LL-37/defensin hBD-2-induced expression of certain NLRs and RLRs, i.e., NOD1, NOD2, and RIG-I, in fully-mature tissue MCs, and the impact of LL-37 and hBD-2 on MC pro-inflammatory activity. All experiments were carried out in vitro on freshly-isolated peritoneal (P)MCs. qRT-PCR, western blotting, flow cytometry, and confocal microscopy were used to evaluate both constitutive and LL-37/hBD-2-induced expression of NOD1, NOD2, and RIG-I receptors. ROS was determined using H(2)DCFDA, and Boyden microchamber assay was used to define the migratory response. Standard techniques assessed histamine, cysLT, and chemokine generation. PMCs express NOD1, NOD2, and RIG-I constitutively. LL-37 and hBD-2 enhance the expression and induce translocation of the studied receptors and directly activate the pro-inflammatory and migratory responses of PMCs. Observations demonstrate that LL-37 and hBD-2 might augment MC capability and sensitivity to NLR and RLR ligands and strengthen the role of MCs in inflammation.
format Online
Article
Text
id pubmed-6079022
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-60790222018-08-09 The RLR/NLR expression and pro-inflammatory activity of tissue mast cells are regulated by cathelicidin LL-37 and defensin hBD-2 Agier, Justyna Różalska, Sylwia Wiktorska, Magdalena Żelechowska, Paulina Pastwińska, Joanna Brzezińska-Błaszczyk, Ewa Sci Rep Article Considering the significance of mast cells (MCs) in the course of various physiological and pathological processes, and the pivotal role of endogenous molecules, i.e., cathelicidins and defensins as multifunctional modulators, the study examines the constitutive and cathelicidin LL-37/defensin hBD-2-induced expression of certain NLRs and RLRs, i.e., NOD1, NOD2, and RIG-I, in fully-mature tissue MCs, and the impact of LL-37 and hBD-2 on MC pro-inflammatory activity. All experiments were carried out in vitro on freshly-isolated peritoneal (P)MCs. qRT-PCR, western blotting, flow cytometry, and confocal microscopy were used to evaluate both constitutive and LL-37/hBD-2-induced expression of NOD1, NOD2, and RIG-I receptors. ROS was determined using H(2)DCFDA, and Boyden microchamber assay was used to define the migratory response. Standard techniques assessed histamine, cysLT, and chemokine generation. PMCs express NOD1, NOD2, and RIG-I constitutively. LL-37 and hBD-2 enhance the expression and induce translocation of the studied receptors and directly activate the pro-inflammatory and migratory responses of PMCs. Observations demonstrate that LL-37 and hBD-2 might augment MC capability and sensitivity to NLR and RLR ligands and strengthen the role of MCs in inflammation. Nature Publishing Group UK 2018-08-06 /pmc/articles/PMC6079022/ /pubmed/30082780 http://dx.doi.org/10.1038/s41598-018-30289-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Agier, Justyna
Różalska, Sylwia
Wiktorska, Magdalena
Żelechowska, Paulina
Pastwińska, Joanna
Brzezińska-Błaszczyk, Ewa
The RLR/NLR expression and pro-inflammatory activity of tissue mast cells are regulated by cathelicidin LL-37 and defensin hBD-2
title The RLR/NLR expression and pro-inflammatory activity of tissue mast cells are regulated by cathelicidin LL-37 and defensin hBD-2
title_full The RLR/NLR expression and pro-inflammatory activity of tissue mast cells are regulated by cathelicidin LL-37 and defensin hBD-2
title_fullStr The RLR/NLR expression and pro-inflammatory activity of tissue mast cells are regulated by cathelicidin LL-37 and defensin hBD-2
title_full_unstemmed The RLR/NLR expression and pro-inflammatory activity of tissue mast cells are regulated by cathelicidin LL-37 and defensin hBD-2
title_short The RLR/NLR expression and pro-inflammatory activity of tissue mast cells are regulated by cathelicidin LL-37 and defensin hBD-2
title_sort rlr/nlr expression and pro-inflammatory activity of tissue mast cells are regulated by cathelicidin ll-37 and defensin hbd-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079022/
https://www.ncbi.nlm.nih.gov/pubmed/30082780
http://dx.doi.org/10.1038/s41598-018-30289-w
work_keys_str_mv AT agierjustyna therlrnlrexpressionandproinflammatoryactivityoftissuemastcellsareregulatedbycathelicidinll37anddefensinhbd2
AT rozalskasylwia therlrnlrexpressionandproinflammatoryactivityoftissuemastcellsareregulatedbycathelicidinll37anddefensinhbd2
AT wiktorskamagdalena therlrnlrexpressionandproinflammatoryactivityoftissuemastcellsareregulatedbycathelicidinll37anddefensinhbd2
AT zelechowskapaulina therlrnlrexpressionandproinflammatoryactivityoftissuemastcellsareregulatedbycathelicidinll37anddefensinhbd2
AT pastwinskajoanna therlrnlrexpressionandproinflammatoryactivityoftissuemastcellsareregulatedbycathelicidinll37anddefensinhbd2
AT brzezinskabłaszczykewa therlrnlrexpressionandproinflammatoryactivityoftissuemastcellsareregulatedbycathelicidinll37anddefensinhbd2
AT agierjustyna rlrnlrexpressionandproinflammatoryactivityoftissuemastcellsareregulatedbycathelicidinll37anddefensinhbd2
AT rozalskasylwia rlrnlrexpressionandproinflammatoryactivityoftissuemastcellsareregulatedbycathelicidinll37anddefensinhbd2
AT wiktorskamagdalena rlrnlrexpressionandproinflammatoryactivityoftissuemastcellsareregulatedbycathelicidinll37anddefensinhbd2
AT zelechowskapaulina rlrnlrexpressionandproinflammatoryactivityoftissuemastcellsareregulatedbycathelicidinll37anddefensinhbd2
AT pastwinskajoanna rlrnlrexpressionandproinflammatoryactivityoftissuemastcellsareregulatedbycathelicidinll37anddefensinhbd2
AT brzezinskabłaszczykewa rlrnlrexpressionandproinflammatoryactivityoftissuemastcellsareregulatedbycathelicidinll37anddefensinhbd2