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Sodium Ferulate Attenuates Lidocaine-Induced Corneal Endothelial Impairment
The introduction of intracameral anaesthesia by injection of lidocaine has become popular in cataract surgery for its inherent potency, rapid onset, tissue penetration, and efficiency. However, intracameral lidocaine causes corneal thickening, opacification, and corneal endothelial cell loss. Herein...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079406/ https://www.ncbi.nlm.nih.gov/pubmed/30116483 http://dx.doi.org/10.1155/2018/4967318 |
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author | Jiang, Guojian Fan, Tingjun |
author_facet | Jiang, Guojian Fan, Tingjun |
author_sort | Jiang, Guojian |
collection | PubMed |
description | The introduction of intracameral anaesthesia by injection of lidocaine has become popular in cataract surgery for its inherent potency, rapid onset, tissue penetration, and efficiency. However, intracameral lidocaine causes corneal thickening, opacification, and corneal endothelial cell loss. Herein, we investigated the effects of lidocaine combined with sodium ferulate, an antioxidant with antiapoptotic and anti-inflammatory properties, on lidocaine-induced damage of corneal endothelia with in vitro experiment of morphological changes and cell viability of cultured human corneal endothelial cells and in vivo investigation of corneal endothelial cell density and central corneal thickness of cat eyes. Our finding indicates that sodium ferulate from 25 to 200 mg/L significantly reduced 2 g/L lidocaine-induced toxicity to human corneal endothelial cells, and 50 mg/L sodium ferulate recovered the damaged human corneal endothelial cells to normal growth status. Furthermore, 100 mg/L sodium ferulate significantly inhibited lidocaine-induced corneal endothelial cell loss and corneal thickening in cat eyes. In conclusion, sodium ferulate protects human corneal endothelial cells from lidocaine-induced cytotoxicity and attenuates corneal endothelial cell loss and central corneal thickening of cat eyes after intracameral injection with lidocaine. It is likely that the antioxidant effect of sodium ferulate reduces the cytotoxic and inflammatory corneal reaction during intracameral anaesthesia. |
format | Online Article Text |
id | pubmed-6079406 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-60794062018-08-16 Sodium Ferulate Attenuates Lidocaine-Induced Corneal Endothelial Impairment Jiang, Guojian Fan, Tingjun Oxid Med Cell Longev Research Article The introduction of intracameral anaesthesia by injection of lidocaine has become popular in cataract surgery for its inherent potency, rapid onset, tissue penetration, and efficiency. However, intracameral lidocaine causes corneal thickening, opacification, and corneal endothelial cell loss. Herein, we investigated the effects of lidocaine combined with sodium ferulate, an antioxidant with antiapoptotic and anti-inflammatory properties, on lidocaine-induced damage of corneal endothelia with in vitro experiment of morphological changes and cell viability of cultured human corneal endothelial cells and in vivo investigation of corneal endothelial cell density and central corneal thickness of cat eyes. Our finding indicates that sodium ferulate from 25 to 200 mg/L significantly reduced 2 g/L lidocaine-induced toxicity to human corneal endothelial cells, and 50 mg/L sodium ferulate recovered the damaged human corneal endothelial cells to normal growth status. Furthermore, 100 mg/L sodium ferulate significantly inhibited lidocaine-induced corneal endothelial cell loss and corneal thickening in cat eyes. In conclusion, sodium ferulate protects human corneal endothelial cells from lidocaine-induced cytotoxicity and attenuates corneal endothelial cell loss and central corneal thickening of cat eyes after intracameral injection with lidocaine. It is likely that the antioxidant effect of sodium ferulate reduces the cytotoxic and inflammatory corneal reaction during intracameral anaesthesia. Hindawi 2018-07-08 /pmc/articles/PMC6079406/ /pubmed/30116483 http://dx.doi.org/10.1155/2018/4967318 Text en Copyright © 2018 Guojian Jiang and Tingjun Fan. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Jiang, Guojian Fan, Tingjun Sodium Ferulate Attenuates Lidocaine-Induced Corneal Endothelial Impairment |
title | Sodium Ferulate Attenuates Lidocaine-Induced Corneal Endothelial Impairment |
title_full | Sodium Ferulate Attenuates Lidocaine-Induced Corneal Endothelial Impairment |
title_fullStr | Sodium Ferulate Attenuates Lidocaine-Induced Corneal Endothelial Impairment |
title_full_unstemmed | Sodium Ferulate Attenuates Lidocaine-Induced Corneal Endothelial Impairment |
title_short | Sodium Ferulate Attenuates Lidocaine-Induced Corneal Endothelial Impairment |
title_sort | sodium ferulate attenuates lidocaine-induced corneal endothelial impairment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079406/ https://www.ncbi.nlm.nih.gov/pubmed/30116483 http://dx.doi.org/10.1155/2018/4967318 |
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