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Coffea canephora Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus

The objective of the present study was to evaluate the antimicrobial activity of the Cc-LTP2 and Cc-GRP peptides isolated from Coffea canephora seeds and their possible synergistic activity with the azole drug fluconazole and characterize their mechanisms of action on cells of pathogenic fungi. Cc-L...

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Autores principales: Bard, Gabriela C. V., Taveira, Gabriel B., Souza, Thaynã A. M., Mello, Érica O., Souza, Sávio B., Ramos, Alessandro C., Carvalho, André O., Pereira, Lídia S., Zottich, Umberto, Rodrigues, Rosana, Gomes, Valdirene M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079426/
https://www.ncbi.nlm.nih.gov/pubmed/30123275
http://dx.doi.org/10.1155/2018/8546470
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author Bard, Gabriela C. V.
Taveira, Gabriel B.
Souza, Thaynã A. M.
Mello, Érica O.
Souza, Sávio B.
Ramos, Alessandro C.
Carvalho, André O.
Pereira, Lídia S.
Zottich, Umberto
Rodrigues, Rosana
Gomes, Valdirene M.
author_facet Bard, Gabriela C. V.
Taveira, Gabriel B.
Souza, Thaynã A. M.
Mello, Érica O.
Souza, Sávio B.
Ramos, Alessandro C.
Carvalho, André O.
Pereira, Lídia S.
Zottich, Umberto
Rodrigues, Rosana
Gomes, Valdirene M.
author_sort Bard, Gabriela C. V.
collection PubMed
description The objective of the present study was to evaluate the antimicrobial activity of the Cc-LTP2 and Cc-GRP peptides isolated from Coffea canephora seeds and their possible synergistic activity with the azole drug fluconazole and characterize their mechanisms of action on cells of pathogenic fungi. Cc-LTP2 and Cc-GRP alone or in combination with 20 µg/mL of fluconazole were evaluated for their antimicrobial activity on the fungus Fusarium solani, and the effects of these peptides on the permeability of membranes and the induction of oxidative stress were determined. Our results show that these peptides at a concentration of 400 µg/mL combined with 20 µg/mL of fluconazole were able to inhibit the growth of the tested fungi, promote changes in their growth pattern, permeabilize the membrane, and induce reactive oxygen species (ROS). Some of these results were also observed with the peptides alone or with fluconazole alone, suggesting that the peptides act synergistically, promoting the potentiation of antimicrobial action. In this study, it was shown that Cc-LTP2 and Cc-GRP in combination with fluconazole were able to inhibit the growth of the fungus F. solani, to promote permeabilization of its membrane, and to induce the production of ROS, suggesting a combinatorial activity between the peptides and fluconazole.
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spelling pubmed-60794262018-08-19 Coffea canephora Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus Bard, Gabriela C. V. Taveira, Gabriel B. Souza, Thaynã A. M. Mello, Érica O. Souza, Sávio B. Ramos, Alessandro C. Carvalho, André O. Pereira, Lídia S. Zottich, Umberto Rodrigues, Rosana Gomes, Valdirene M. Int J Microbiol Research Article The objective of the present study was to evaluate the antimicrobial activity of the Cc-LTP2 and Cc-GRP peptides isolated from Coffea canephora seeds and their possible synergistic activity with the azole drug fluconazole and characterize their mechanisms of action on cells of pathogenic fungi. Cc-LTP2 and Cc-GRP alone or in combination with 20 µg/mL of fluconazole were evaluated for their antimicrobial activity on the fungus Fusarium solani, and the effects of these peptides on the permeability of membranes and the induction of oxidative stress were determined. Our results show that these peptides at a concentration of 400 µg/mL combined with 20 µg/mL of fluconazole were able to inhibit the growth of the tested fungi, promote changes in their growth pattern, permeabilize the membrane, and induce reactive oxygen species (ROS). Some of these results were also observed with the peptides alone or with fluconazole alone, suggesting that the peptides act synergistically, promoting the potentiation of antimicrobial action. In this study, it was shown that Cc-LTP2 and Cc-GRP in combination with fluconazole were able to inhibit the growth of the fungus F. solani, to promote permeabilization of its membrane, and to induce the production of ROS, suggesting a combinatorial activity between the peptides and fluconazole. Hindawi 2018-07-10 /pmc/articles/PMC6079426/ /pubmed/30123275 http://dx.doi.org/10.1155/2018/8546470 Text en Copyright © 2018 Gabriela C. V. Bard et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bard, Gabriela C. V.
Taveira, Gabriel B.
Souza, Thaynã A. M.
Mello, Érica O.
Souza, Sávio B.
Ramos, Alessandro C.
Carvalho, André O.
Pereira, Lídia S.
Zottich, Umberto
Rodrigues, Rosana
Gomes, Valdirene M.
Coffea canephora Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus
title Coffea canephora Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus
title_full Coffea canephora Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus
title_fullStr Coffea canephora Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus
title_full_unstemmed Coffea canephora Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus
title_short Coffea canephora Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus
title_sort coffea canephora peptides in combinatorial treatment with fluconazole: antimicrobial activity against phytopathogenic fungus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079426/
https://www.ncbi.nlm.nih.gov/pubmed/30123275
http://dx.doi.org/10.1155/2018/8546470
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