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Adipocyte DIO2 Expression Increases in Human Obesity but Is Not Related to Systemic Insulin Sensitivity

Deiodinase type II (D2), encoded by DIO2, catalyzes the conversion of T4 to bioactive T3. T3 not only stimulates adaptive thermogenesis but also affects adipose tissue (AT) lipid accumulation, mitochondrial function, inflammation, and potentially systemic metabolism. Although better defined in brown...

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Autores principales: Bradley, David, Liu, Joey, Blaszczak, Alecia, Wright, Valerie, Jalilvand, Anahita, Needleman, Bradley, Noria, Sabrena, Renton, David, Hsueh, Willa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079440/
https://www.ncbi.nlm.nih.gov/pubmed/30116736
http://dx.doi.org/10.1155/2018/2464652
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author Bradley, David
Liu, Joey
Blaszczak, Alecia
Wright, Valerie
Jalilvand, Anahita
Needleman, Bradley
Noria, Sabrena
Renton, David
Hsueh, Willa
author_facet Bradley, David
Liu, Joey
Blaszczak, Alecia
Wright, Valerie
Jalilvand, Anahita
Needleman, Bradley
Noria, Sabrena
Renton, David
Hsueh, Willa
author_sort Bradley, David
collection PubMed
description Deiodinase type II (D2), encoded by DIO2, catalyzes the conversion of T4 to bioactive T3. T3 not only stimulates adaptive thermogenesis but also affects adipose tissue (AT) lipid accumulation, mitochondrial function, inflammation, and potentially systemic metabolism. Although better defined in brown AT, the precise role of DIO2 expression in white AT remains largely unknown, with data derived only from whole fat. Therefore, the purpose of this study was to determine whether subcutaneous (SAT) and visceral (VAT) adipocyte-specific gene expression of DIO2 differs between obese and lean patients and whether these differences relate to alterations in mitochondrial function, fatty acid flux, inflammatory cytokines/adipokines, and ultimately insulin sensitivity. Accordingly, adipocytes of 73 obese and 21 lean subjects were isolated and subjected to gene expression analyses. Our results demonstrate that obese compared to lean human individuals have increased adipocyte-specific DIO2 expression in both SAT and VAT. Although higher DIO2 was strongly related to reduced fatty acid synthesis/oxidation and mitochondrial function, we found no relationship to proinflammatory cytokines or insulin resistance and no difference based on diabetic status. Our results suggest that adipocyte-derived DIO2 may play a role in weight maintenance but is likely not a major contributor to obesity-related insulin resistance.
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spelling pubmed-60794402018-08-16 Adipocyte DIO2 Expression Increases in Human Obesity but Is Not Related to Systemic Insulin Sensitivity Bradley, David Liu, Joey Blaszczak, Alecia Wright, Valerie Jalilvand, Anahita Needleman, Bradley Noria, Sabrena Renton, David Hsueh, Willa J Diabetes Res Research Article Deiodinase type II (D2), encoded by DIO2, catalyzes the conversion of T4 to bioactive T3. T3 not only stimulates adaptive thermogenesis but also affects adipose tissue (AT) lipid accumulation, mitochondrial function, inflammation, and potentially systemic metabolism. Although better defined in brown AT, the precise role of DIO2 expression in white AT remains largely unknown, with data derived only from whole fat. Therefore, the purpose of this study was to determine whether subcutaneous (SAT) and visceral (VAT) adipocyte-specific gene expression of DIO2 differs between obese and lean patients and whether these differences relate to alterations in mitochondrial function, fatty acid flux, inflammatory cytokines/adipokines, and ultimately insulin sensitivity. Accordingly, adipocytes of 73 obese and 21 lean subjects were isolated and subjected to gene expression analyses. Our results demonstrate that obese compared to lean human individuals have increased adipocyte-specific DIO2 expression in both SAT and VAT. Although higher DIO2 was strongly related to reduced fatty acid synthesis/oxidation and mitochondrial function, we found no relationship to proinflammatory cytokines or insulin resistance and no difference based on diabetic status. Our results suggest that adipocyte-derived DIO2 may play a role in weight maintenance but is likely not a major contributor to obesity-related insulin resistance. Hindawi 2018-07-15 /pmc/articles/PMC6079440/ /pubmed/30116736 http://dx.doi.org/10.1155/2018/2464652 Text en Copyright © 2018 David Bradley et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bradley, David
Liu, Joey
Blaszczak, Alecia
Wright, Valerie
Jalilvand, Anahita
Needleman, Bradley
Noria, Sabrena
Renton, David
Hsueh, Willa
Adipocyte DIO2 Expression Increases in Human Obesity but Is Not Related to Systemic Insulin Sensitivity
title Adipocyte DIO2 Expression Increases in Human Obesity but Is Not Related to Systemic Insulin Sensitivity
title_full Adipocyte DIO2 Expression Increases in Human Obesity but Is Not Related to Systemic Insulin Sensitivity
title_fullStr Adipocyte DIO2 Expression Increases in Human Obesity but Is Not Related to Systemic Insulin Sensitivity
title_full_unstemmed Adipocyte DIO2 Expression Increases in Human Obesity but Is Not Related to Systemic Insulin Sensitivity
title_short Adipocyte DIO2 Expression Increases in Human Obesity but Is Not Related to Systemic Insulin Sensitivity
title_sort adipocyte dio2 expression increases in human obesity but is not related to systemic insulin sensitivity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079440/
https://www.ncbi.nlm.nih.gov/pubmed/30116736
http://dx.doi.org/10.1155/2018/2464652
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