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MicroRNAs as Urinary Biomarker for Oncocytoma

The identification of benign renal oncocytoma, its differentiation from malignant renal tumors, and their eosinophilic variants are a continuous challenge, influencing preoperative planning and being an unnecessary stress factor for patients. Regressive changes enhance the diagnostic dilemma, making...

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Autores principales: von Brandenstein, Melanie, Schlosser, Monika, Herden, Jan, Heidenreich, Axel, Störkel, Stefan, Fries, Jochen W. U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079495/
https://www.ncbi.nlm.nih.gov/pubmed/30116406
http://dx.doi.org/10.1155/2018/6979073
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author von Brandenstein, Melanie
Schlosser, Monika
Herden, Jan
Heidenreich, Axel
Störkel, Stefan
Fries, Jochen W. U.
author_facet von Brandenstein, Melanie
Schlosser, Monika
Herden, Jan
Heidenreich, Axel
Störkel, Stefan
Fries, Jochen W. U.
author_sort von Brandenstein, Melanie
collection PubMed
description The identification of benign renal oncocytoma, its differentiation from malignant renal tumors, and their eosinophilic variants are a continuous challenge, influencing preoperative planning and being an unnecessary stress factor for patients. Regressive changes enhance the diagnostic dilemma, making evaluations by frozen sections or by immunohistology (on biopsies) unreliable. MicroRNAs (miRs) have been proposed as novel biomarkers to differentiate renal tumor subtypes. However, their value as a diagnostic biomarker of oncocytoma in urines based on mechanisms known in oncocytomas has not been exploited. We used urines from patients with renal tumors (oncocytoma, renal cell carcinoma: clear cell, papillary, chromophobe) and with other urogenital lesions. miRs were extracted and detected via qRT-PCR, the respective tumors analyzed by immunohistology. We found isocitrate dehydrogenase 2 upregulated in oncocytoma and oncocytic chromophobe carcinoma, indicating an increased Krebs cycle metabolism. Since we had shown that all renal tumors are stimulated by endothelin-1, we analyzed miRs preidentified by microarray after endothelin-1 stimulation of renal epithelial cells. Four miRs are proposed as presurgical urinary biomarkers due to their known regulatory mechanism in oncocytoma: miR-498 (formation of the oncocytoma-specific slice-form of vimentin, Vim3), miR-183 (associated with increased CO(2) levels), miR-205, and miR-31 (signaling through downregulation of PKC epsilon, shown previously).
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spelling pubmed-60794952018-08-16 MicroRNAs as Urinary Biomarker for Oncocytoma von Brandenstein, Melanie Schlosser, Monika Herden, Jan Heidenreich, Axel Störkel, Stefan Fries, Jochen W. U. Dis Markers Research Article The identification of benign renal oncocytoma, its differentiation from malignant renal tumors, and their eosinophilic variants are a continuous challenge, influencing preoperative planning and being an unnecessary stress factor for patients. Regressive changes enhance the diagnostic dilemma, making evaluations by frozen sections or by immunohistology (on biopsies) unreliable. MicroRNAs (miRs) have been proposed as novel biomarkers to differentiate renal tumor subtypes. However, their value as a diagnostic biomarker of oncocytoma in urines based on mechanisms known in oncocytomas has not been exploited. We used urines from patients with renal tumors (oncocytoma, renal cell carcinoma: clear cell, papillary, chromophobe) and with other urogenital lesions. miRs were extracted and detected via qRT-PCR, the respective tumors analyzed by immunohistology. We found isocitrate dehydrogenase 2 upregulated in oncocytoma and oncocytic chromophobe carcinoma, indicating an increased Krebs cycle metabolism. Since we had shown that all renal tumors are stimulated by endothelin-1, we analyzed miRs preidentified by microarray after endothelin-1 stimulation of renal epithelial cells. Four miRs are proposed as presurgical urinary biomarkers due to their known regulatory mechanism in oncocytoma: miR-498 (formation of the oncocytoma-specific slice-form of vimentin, Vim3), miR-183 (associated with increased CO(2) levels), miR-205, and miR-31 (signaling through downregulation of PKC epsilon, shown previously). Hindawi 2018-07-16 /pmc/articles/PMC6079495/ /pubmed/30116406 http://dx.doi.org/10.1155/2018/6979073 Text en Copyright © 2018 Melanie von Brandenstein et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
von Brandenstein, Melanie
Schlosser, Monika
Herden, Jan
Heidenreich, Axel
Störkel, Stefan
Fries, Jochen W. U.
MicroRNAs as Urinary Biomarker for Oncocytoma
title MicroRNAs as Urinary Biomarker for Oncocytoma
title_full MicroRNAs as Urinary Biomarker for Oncocytoma
title_fullStr MicroRNAs as Urinary Biomarker for Oncocytoma
title_full_unstemmed MicroRNAs as Urinary Biomarker for Oncocytoma
title_short MicroRNAs as Urinary Biomarker for Oncocytoma
title_sort micrornas as urinary biomarker for oncocytoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079495/
https://www.ncbi.nlm.nih.gov/pubmed/30116406
http://dx.doi.org/10.1155/2018/6979073
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