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Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns

Neonatal systemic inflammatory response and multiple organ dysfunction syndrome are the main postnatal insults influencing mortality and morbidity. Critically ill newborns with high predicted mortality are supported by extracorporeal membrane oxygenation (ECMO). Biomarkers of inflammatory response a...

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Autores principales: Vítková, Veronika, Pánek, Martin, Janec, Petr, Šibíková, Michaela, Vobruba, Václav, Haluzík, Martin, Živný, Jan, Janota, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079510/
https://www.ncbi.nlm.nih.gov/pubmed/30116143
http://dx.doi.org/10.1155/2018/1975056
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author Vítková, Veronika
Pánek, Martin
Janec, Petr
Šibíková, Michaela
Vobruba, Václav
Haluzík, Martin
Živný, Jan
Janota, Jan
author_facet Vítková, Veronika
Pánek, Martin
Janec, Petr
Šibíková, Michaela
Vobruba, Václav
Haluzík, Martin
Živný, Jan
Janota, Jan
author_sort Vítková, Veronika
collection PubMed
description Neonatal systemic inflammatory response and multiple organ dysfunction syndrome are the main postnatal insults influencing mortality and morbidity. Critically ill newborns with high predicted mortality are supported by extracorporeal membrane oxygenation (ECMO). Biomarkers of inflammatory response and endothelial injury can be used for early diagnosis and treatment of critical neonatal situations. The aim of our study was to explore plasma proteins and endothelial microvesicles as markers of inflammation and endothelial activation in newborns on ECMO and to compare them with healthy neonates. Thirteen newborns on ECMO and 13 healthy newborns were included in the study. Plasma soluble biomarkers were measured using multiplex immunoassay based on Luminex® xMAP multianalyte profiling platform. The total microvesicle count and plasma level of surface antigen-specific microvesicles were determined by flow cytometry. The plasma concentration of cell-derived microvesicles was measured using annexin-V labeling, and the endothelial origin of microvesicles was determined using lineage-specific antigen labeling of endothelial cell/microvesicle markers (endoglin/CD105, PECAM1/CD31, VEGFR2/CD309, and MadCAM1). Inflammatory markers (procalcitonin, IL-1β, IL-6, and IL-22) were increased in the ECMO group (P < 0.01). The assessment of endothelial markers showed higher concentrations of endocan and angiopoietin-2 (P < 0.01) in the ECMO group while VEGF in the ECMO group was significantly lower (P < 0.01). In the ECMO group, the concentration of annexin-V-positive microvesicles (total microvesicles) and endothelial microvesicles expressing mucosal vascular addressin cell adhesion molecule 1 (MadCAM1) was increased (P = 0.05). In summary, we found increased concentrations of soluble inflammatory and endothelial markers in the plasma of critically ill newborns with multiple organ dysfunction. Increased plasma concentrations of microvesicles may reflect the activation or damage of blood cells and vasculature including endothelial cells. The measurement of cell membrane-derived microvesicles may be added to the panel of established inflammatory markers in order to increase the sensitivity and specificity of the diagnostic process in critically ill newborns.
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spelling pubmed-60795102018-08-16 Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns Vítková, Veronika Pánek, Martin Janec, Petr Šibíková, Michaela Vobruba, Václav Haluzík, Martin Živný, Jan Janota, Jan Mediators Inflamm Research Article Neonatal systemic inflammatory response and multiple organ dysfunction syndrome are the main postnatal insults influencing mortality and morbidity. Critically ill newborns with high predicted mortality are supported by extracorporeal membrane oxygenation (ECMO). Biomarkers of inflammatory response and endothelial injury can be used for early diagnosis and treatment of critical neonatal situations. The aim of our study was to explore plasma proteins and endothelial microvesicles as markers of inflammation and endothelial activation in newborns on ECMO and to compare them with healthy neonates. Thirteen newborns on ECMO and 13 healthy newborns were included in the study. Plasma soluble biomarkers were measured using multiplex immunoassay based on Luminex® xMAP multianalyte profiling platform. The total microvesicle count and plasma level of surface antigen-specific microvesicles were determined by flow cytometry. The plasma concentration of cell-derived microvesicles was measured using annexin-V labeling, and the endothelial origin of microvesicles was determined using lineage-specific antigen labeling of endothelial cell/microvesicle markers (endoglin/CD105, PECAM1/CD31, VEGFR2/CD309, and MadCAM1). Inflammatory markers (procalcitonin, IL-1β, IL-6, and IL-22) were increased in the ECMO group (P < 0.01). The assessment of endothelial markers showed higher concentrations of endocan and angiopoietin-2 (P < 0.01) in the ECMO group while VEGF in the ECMO group was significantly lower (P < 0.01). In the ECMO group, the concentration of annexin-V-positive microvesicles (total microvesicles) and endothelial microvesicles expressing mucosal vascular addressin cell adhesion molecule 1 (MadCAM1) was increased (P = 0.05). In summary, we found increased concentrations of soluble inflammatory and endothelial markers in the plasma of critically ill newborns with multiple organ dysfunction. Increased plasma concentrations of microvesicles may reflect the activation or damage of blood cells and vasculature including endothelial cells. The measurement of cell membrane-derived microvesicles may be added to the panel of established inflammatory markers in order to increase the sensitivity and specificity of the diagnostic process in critically ill newborns. Hindawi 2018-07-19 /pmc/articles/PMC6079510/ /pubmed/30116143 http://dx.doi.org/10.1155/2018/1975056 Text en Copyright © 2018 Veronika Vítková et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Vítková, Veronika
Pánek, Martin
Janec, Petr
Šibíková, Michaela
Vobruba, Václav
Haluzík, Martin
Živný, Jan
Janota, Jan
Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns
title Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns
title_full Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns
title_fullStr Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns
title_full_unstemmed Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns
title_short Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns
title_sort endothelial microvesicles and soluble markers of endothelial injury in critically ill newborns
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079510/
https://www.ncbi.nlm.nih.gov/pubmed/30116143
http://dx.doi.org/10.1155/2018/1975056
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