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Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis?

OBJECTIVES: The aim of this study was to determine the level of five different pro- and anti-inflammatory cytokines to study the inflammatory response of leptospirosis. MATERIALS AND METHODS: The serum cytokine levels of IL-10, IL-17A, IL-21, IL-23, and TNF-α were investigated in 57 patients with le...

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Autores principales: Bandara, Kanchana, Gunasekara, Chinthika, Weerasekera, Manjula, Marasinghe, Chamil, Ranasinghe, Nilantha, Fernando, Neluka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079516/
https://www.ncbi.nlm.nih.gov/pubmed/30123395
http://dx.doi.org/10.1155/2018/9704532
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author Bandara, Kanchana
Gunasekara, Chinthika
Weerasekera, Manjula
Marasinghe, Chamil
Ranasinghe, Nilantha
Fernando, Neluka
author_facet Bandara, Kanchana
Gunasekara, Chinthika
Weerasekera, Manjula
Marasinghe, Chamil
Ranasinghe, Nilantha
Fernando, Neluka
author_sort Bandara, Kanchana
collection PubMed
description OBJECTIVES: The aim of this study was to determine the level of five different pro- and anti-inflammatory cytokines to study the inflammatory response of leptospirosis. MATERIALS AND METHODS: The serum cytokine levels of IL-10, IL-17A, IL-21, IL-23, and TNF-α were investigated in 57 patients with leptospirosis and 12 healthy controls using a commercially available ELISA kit (Mabtech, Sweden). Statistical analysis was done using Graphpad Prism. RESULTS: Elevation of serum IL-10 and IL-17A levels and significant elevation of serum IL-21 (p=0.002), IL-23 (p=0.002), and TNF-α (p=0.039) were observed among leptospirosis patients compared to the healthy control group. The two major complications observed among these patients were renal failure and liver involvement. Renal failure was significantly associated with elevation of IL-21 and IL-23, while patients with liver involvement had a significant elevation of IL-21, IL-23, and TNF-α. CONCLUSION: Elevation of IL-17A together with the significant elevation of IL-21 and IL-23 suggests a possible involvement of Th17 cells in the immunopathogenesis of leptospirosis.
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spelling pubmed-60795162018-08-19 Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis? Bandara, Kanchana Gunasekara, Chinthika Weerasekera, Manjula Marasinghe, Chamil Ranasinghe, Nilantha Fernando, Neluka Can J Infect Dis Med Microbiol Research Article OBJECTIVES: The aim of this study was to determine the level of five different pro- and anti-inflammatory cytokines to study the inflammatory response of leptospirosis. MATERIALS AND METHODS: The serum cytokine levels of IL-10, IL-17A, IL-21, IL-23, and TNF-α were investigated in 57 patients with leptospirosis and 12 healthy controls using a commercially available ELISA kit (Mabtech, Sweden). Statistical analysis was done using Graphpad Prism. RESULTS: Elevation of serum IL-10 and IL-17A levels and significant elevation of serum IL-21 (p=0.002), IL-23 (p=0.002), and TNF-α (p=0.039) were observed among leptospirosis patients compared to the healthy control group. The two major complications observed among these patients were renal failure and liver involvement. Renal failure was significantly associated with elevation of IL-21 and IL-23, while patients with liver involvement had a significant elevation of IL-21, IL-23, and TNF-α. CONCLUSION: Elevation of IL-17A together with the significant elevation of IL-21 and IL-23 suggests a possible involvement of Th17 cells in the immunopathogenesis of leptospirosis. Hindawi 2018-07-10 /pmc/articles/PMC6079516/ /pubmed/30123395 http://dx.doi.org/10.1155/2018/9704532 Text en Copyright © 2018 Kanchana Bandara et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bandara, Kanchana
Gunasekara, Chinthika
Weerasekera, Manjula
Marasinghe, Chamil
Ranasinghe, Nilantha
Fernando, Neluka
Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis?
title Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis?
title_full Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis?
title_fullStr Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis?
title_full_unstemmed Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis?
title_short Do the Th17 Cells Play a Role in the Pathogenesis of Leptospirosis?
title_sort do the th17 cells play a role in the pathogenesis of leptospirosis?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079516/
https://www.ncbi.nlm.nih.gov/pubmed/30123395
http://dx.doi.org/10.1155/2018/9704532
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