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Cryoprotectant-free high-pressure cooling and dynamic nuclear polarization for more sensitive detection of hydrogen in neutron protein crystallography
To improve the sensitivity of hydrogen detection using neutrons, a proton-polarization technique together with a high-pressure cooling method is necessary. The highest pressure (200 MPa) used in the experiment described here enabled relatively large protein crystals to be cooled without any cryoprot...
Autores principales: | Tanaka, Ichiro, Komatsuzaki, Naoya, Yue, Wen-Xue, Chatake, Toshiyuki, Kusaka, Katsuhiro, Niimura, Nobuo, Miura, Daisuke, Iwata, Takahiro, Miyachi, Yoshiyuki, Nukazuka, Genki, Matsuda, Hiroki |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079630/ https://www.ncbi.nlm.nih.gov/pubmed/30082514 http://dx.doi.org/10.1107/S2059798318005028 |
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