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The kinase domain of CK1 enzymes contains the localization cue essential for compartmentalized signaling at the spindle pole

CK1 protein kinases contribute to multiple biological processes, but how they are tailored to function in compartmentalized signaling events is largely unknown. Hhp1 and Hhp2 (Hhp1/2) are the soluble CK1 family members in Schizosaccharomyces pombe. One of their functions is to inhibit the septation...

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Autores principales: Elmore, Zachary C., Guillen, Rodrigo X., Gould, Kathleen L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080649/
https://www.ncbi.nlm.nih.gov/pubmed/29742018
http://dx.doi.org/10.1091/mbc.E18-02-0129
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author Elmore, Zachary C.
Guillen, Rodrigo X.
Gould, Kathleen L.
author_facet Elmore, Zachary C.
Guillen, Rodrigo X.
Gould, Kathleen L.
author_sort Elmore, Zachary C.
collection PubMed
description CK1 protein kinases contribute to multiple biological processes, but how they are tailored to function in compartmentalized signaling events is largely unknown. Hhp1 and Hhp2 (Hhp1/2) are the soluble CK1 family members in Schizosaccharomyces pombe. One of their functions is to inhibit the septation initiation network (SIN) during a mitotic checkpoint arrest. The SIN is assembled by Sid4 at spindle pole bodies (SPBs), and though Hhp1/2 colocalize there, it is not known how they are targeted there or whether their SPB localization is required for SIN inhibition. Here, we establish that Hhp1/2 localize throughout the cell cycle to SPBs, as well as to the nucleus, cell tips, and division site. We find that their catalytic domains but not their enzymatic function are used for SPB targeting and that this targeting strategy is conserved in human CK1δ/ε localization to centrosomes. Further, we pinpoint amino acids in the Hhp1 catalytic domain required for SPB interaction; mutation of these residues disrupts Hhp1 association with the core SPB protein Ppc89, and the inhibition of cytokinesis in the setting of spindle stress. Taken together, these data have enabled us to define a molecular mechanism used by CK1 enzymes to target a specific cellular locale for compartmentalized signaling.
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spelling pubmed-60806492018-09-16 The kinase domain of CK1 enzymes contains the localization cue essential for compartmentalized signaling at the spindle pole Elmore, Zachary C. Guillen, Rodrigo X. Gould, Kathleen L. Mol Biol Cell Articles CK1 protein kinases contribute to multiple biological processes, but how they are tailored to function in compartmentalized signaling events is largely unknown. Hhp1 and Hhp2 (Hhp1/2) are the soluble CK1 family members in Schizosaccharomyces pombe. One of their functions is to inhibit the septation initiation network (SIN) during a mitotic checkpoint arrest. The SIN is assembled by Sid4 at spindle pole bodies (SPBs), and though Hhp1/2 colocalize there, it is not known how they are targeted there or whether their SPB localization is required for SIN inhibition. Here, we establish that Hhp1/2 localize throughout the cell cycle to SPBs, as well as to the nucleus, cell tips, and division site. We find that their catalytic domains but not their enzymatic function are used for SPB targeting and that this targeting strategy is conserved in human CK1δ/ε localization to centrosomes. Further, we pinpoint amino acids in the Hhp1 catalytic domain required for SPB interaction; mutation of these residues disrupts Hhp1 association with the core SPB protein Ppc89, and the inhibition of cytokinesis in the setting of spindle stress. Taken together, these data have enabled us to define a molecular mechanism used by CK1 enzymes to target a specific cellular locale for compartmentalized signaling. The American Society for Cell Biology 2018-07-01 /pmc/articles/PMC6080649/ /pubmed/29742018 http://dx.doi.org/10.1091/mbc.E18-02-0129 Text en © 2018 Elmore et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Elmore, Zachary C.
Guillen, Rodrigo X.
Gould, Kathleen L.
The kinase domain of CK1 enzymes contains the localization cue essential for compartmentalized signaling at the spindle pole
title The kinase domain of CK1 enzymes contains the localization cue essential for compartmentalized signaling at the spindle pole
title_full The kinase domain of CK1 enzymes contains the localization cue essential for compartmentalized signaling at the spindle pole
title_fullStr The kinase domain of CK1 enzymes contains the localization cue essential for compartmentalized signaling at the spindle pole
title_full_unstemmed The kinase domain of CK1 enzymes contains the localization cue essential for compartmentalized signaling at the spindle pole
title_short The kinase domain of CK1 enzymes contains the localization cue essential for compartmentalized signaling at the spindle pole
title_sort kinase domain of ck1 enzymes contains the localization cue essential for compartmentalized signaling at the spindle pole
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080649/
https://www.ncbi.nlm.nih.gov/pubmed/29742018
http://dx.doi.org/10.1091/mbc.E18-02-0129
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