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A beginner’s guide to rigor and reproducibility in fluorescence imaging experiments

Fluorescence light microscopy is an indispensable approach for the investigation of cell biological mechanisms. With the development of cutting-edge tools such as genetically encoded fluorescent proteins and superresolution methods, light microscopy is more powerful than ever at providing insight in...

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Detalles Bibliográficos
Autores principales: Lee, Jen-Yi, Kitaoka, Maiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080651/
https://www.ncbi.nlm.nih.gov/pubmed/29953344
http://dx.doi.org/10.1091/mbc.E17-05-0276
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author Lee, Jen-Yi
Kitaoka, Maiko
author_facet Lee, Jen-Yi
Kitaoka, Maiko
author_sort Lee, Jen-Yi
collection PubMed
description Fluorescence light microscopy is an indispensable approach for the investigation of cell biological mechanisms. With the development of cutting-edge tools such as genetically encoded fluorescent proteins and superresolution methods, light microscopy is more powerful than ever at providing insight into a broad range of phenomena, from bacterial fission to cancer metastasis. However, as with all experimental approaches, care must be taken to ensure reliable and reproducible data collection, analysis, and reporting. Each step of every imaging experiment, from design to execution to communication to data management, should be critically assessed for bias, rigor, and reproducibility. This Perspective provides a basic “best practices” guide for designing and executing fluorescence imaging experiments, with the goal of introducing researchers to concepts that will help empower them to acquire images with rigor.
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spelling pubmed-60806512018-09-16 A beginner’s guide to rigor and reproducibility in fluorescence imaging experiments Lee, Jen-Yi Kitaoka, Maiko Mol Biol Cell MBoC Technical Perspectives Fluorescence light microscopy is an indispensable approach for the investigation of cell biological mechanisms. With the development of cutting-edge tools such as genetically encoded fluorescent proteins and superresolution methods, light microscopy is more powerful than ever at providing insight into a broad range of phenomena, from bacterial fission to cancer metastasis. However, as with all experimental approaches, care must be taken to ensure reliable and reproducible data collection, analysis, and reporting. Each step of every imaging experiment, from design to execution to communication to data management, should be critically assessed for bias, rigor, and reproducibility. This Perspective provides a basic “best practices” guide for designing and executing fluorescence imaging experiments, with the goal of introducing researchers to concepts that will help empower them to acquire images with rigor. The American Society for Cell Biology 2018-07-01 /pmc/articles/PMC6080651/ /pubmed/29953344 http://dx.doi.org/10.1091/mbc.E17-05-0276 Text en © 2018 Lee and Kitaoka. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle MBoC Technical Perspectives
Lee, Jen-Yi
Kitaoka, Maiko
A beginner’s guide to rigor and reproducibility in fluorescence imaging experiments
title A beginner’s guide to rigor and reproducibility in fluorescence imaging experiments
title_full A beginner’s guide to rigor and reproducibility in fluorescence imaging experiments
title_fullStr A beginner’s guide to rigor and reproducibility in fluorescence imaging experiments
title_full_unstemmed A beginner’s guide to rigor and reproducibility in fluorescence imaging experiments
title_short A beginner’s guide to rigor and reproducibility in fluorescence imaging experiments
title_sort beginner’s guide to rigor and reproducibility in fluorescence imaging experiments
topic MBoC Technical Perspectives
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080651/
https://www.ncbi.nlm.nih.gov/pubmed/29953344
http://dx.doi.org/10.1091/mbc.E17-05-0276
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