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Extracellular vesicles direct migration by synthesizing and releasing chemotactic signals
Chemotactic signals are relayed to neighboring cells through the secretion of additional chemoattractants. We previously showed in Dictyostelium discoideum that the adenylyl cyclase A, which synthesizes the chemoattractant cyclic adenosine monophosphate (cAMP), is present in the intraluminal vesicle...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080930/ https://www.ncbi.nlm.nih.gov/pubmed/29884750 http://dx.doi.org/10.1083/jcb.201710170 |
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author | Kriebel, Paul W. Majumdar, Ritankar Jenkins, Lisa M. Senoo, Hiroshi Wang, Weiye Ammu, Sonia Chen, Song Narayan, Kedar Iijima, Miho Parent, Carole A. |
author_facet | Kriebel, Paul W. Majumdar, Ritankar Jenkins, Lisa M. Senoo, Hiroshi Wang, Weiye Ammu, Sonia Chen, Song Narayan, Kedar Iijima, Miho Parent, Carole A. |
author_sort | Kriebel, Paul W. |
collection | PubMed |
description | Chemotactic signals are relayed to neighboring cells through the secretion of additional chemoattractants. We previously showed in Dictyostelium discoideum that the adenylyl cyclase A, which synthesizes the chemoattractant cyclic adenosine monophosphate (cAMP), is present in the intraluminal vesicles of multivesicular bodies (MVBs) that coalesce at the back of cells. Using ultrastructural reconstructions, we now show that ACA-containing MVBs release their contents to attract neighboring cells. We show that the released vesicles are capable of directing migration and streaming and are central to chemotactic signal relay. We demonstrate that the released vesicles not only contain cAMP but also can actively synthesize and release cAMP to promote chemotaxis. Through proteomic, pharmacological, and genetic approaches, we determined that the vesicular cAMP is released via the ABCC8 transporter. Together, our findings show that extracellular vesicles released by D. discoideum cells are functional entities that mediate signal relay during chemotaxis and streaming. |
format | Online Article Text |
id | pubmed-6080930 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-60809302019-02-06 Extracellular vesicles direct migration by synthesizing and releasing chemotactic signals Kriebel, Paul W. Majumdar, Ritankar Jenkins, Lisa M. Senoo, Hiroshi Wang, Weiye Ammu, Sonia Chen, Song Narayan, Kedar Iijima, Miho Parent, Carole A. J Cell Biol Research Articles Chemotactic signals are relayed to neighboring cells through the secretion of additional chemoattractants. We previously showed in Dictyostelium discoideum that the adenylyl cyclase A, which synthesizes the chemoattractant cyclic adenosine monophosphate (cAMP), is present in the intraluminal vesicles of multivesicular bodies (MVBs) that coalesce at the back of cells. Using ultrastructural reconstructions, we now show that ACA-containing MVBs release their contents to attract neighboring cells. We show that the released vesicles are capable of directing migration and streaming and are central to chemotactic signal relay. We demonstrate that the released vesicles not only contain cAMP but also can actively synthesize and release cAMP to promote chemotaxis. Through proteomic, pharmacological, and genetic approaches, we determined that the vesicular cAMP is released via the ABCC8 transporter. Together, our findings show that extracellular vesicles released by D. discoideum cells are functional entities that mediate signal relay during chemotaxis and streaming. Rockefeller University Press 2018-08-06 /pmc/articles/PMC6080930/ /pubmed/29884750 http://dx.doi.org/10.1083/jcb.201710170 Text en © 2018 Kriebel et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Kriebel, Paul W. Majumdar, Ritankar Jenkins, Lisa M. Senoo, Hiroshi Wang, Weiye Ammu, Sonia Chen, Song Narayan, Kedar Iijima, Miho Parent, Carole A. Extracellular vesicles direct migration by synthesizing and releasing chemotactic signals |
title | Extracellular vesicles direct migration by synthesizing and releasing chemotactic signals |
title_full | Extracellular vesicles direct migration by synthesizing and releasing chemotactic signals |
title_fullStr | Extracellular vesicles direct migration by synthesizing and releasing chemotactic signals |
title_full_unstemmed | Extracellular vesicles direct migration by synthesizing and releasing chemotactic signals |
title_short | Extracellular vesicles direct migration by synthesizing and releasing chemotactic signals |
title_sort | extracellular vesicles direct migration by synthesizing and releasing chemotactic signals |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080930/ https://www.ncbi.nlm.nih.gov/pubmed/29884750 http://dx.doi.org/10.1083/jcb.201710170 |
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