Cargando…

Following laser induced changes of plant phenylpropanoids by Raman microscopy

Raman microscopy is a powerful imaging technique for biological materials providing information about chemistry in context with microstructure. A 532 nm laser is often used as excitation source, because high spatial resolution and signal intensity can be achieved. The latter can be controlled by las...

Descripción completa

Detalles Bibliográficos
Autores principales: Prats-Mateu, Batirtze, Bock, Peter, Schroffenegger, Martina, Toca-Herrera, José Luis, Gierlinger, Notburga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6081397/
https://www.ncbi.nlm.nih.gov/pubmed/30087373
http://dx.doi.org/10.1038/s41598-018-30096-3
_version_ 1783345640106885120
author Prats-Mateu, Batirtze
Bock, Peter
Schroffenegger, Martina
Toca-Herrera, José Luis
Gierlinger, Notburga
author_facet Prats-Mateu, Batirtze
Bock, Peter
Schroffenegger, Martina
Toca-Herrera, José Luis
Gierlinger, Notburga
author_sort Prats-Mateu, Batirtze
collection PubMed
description Raman microscopy is a powerful imaging technique for biological materials providing information about chemistry in context with microstructure. A 532 nm laser is often used as excitation source, because high spatial resolution and signal intensity can be achieved. The latter can be controlled by laser power and integration time, whereby high power and long times give good signal to noise ratio. However, most biological materials absorb in the VIS range and fluorescence masking the signal or even sample degradation might be hindering. Here, we show that on lignified plant cell walls even very short integration times and low laser powers induce a change in the ratio of the lignin bands at 1660 and 1600 cm(−1). Time series on lignin model compounds revealed this change only in aromatic molecules with two OH-groups, such as coniferyl alcohol. Therefore, we conclude that monolignols are present in the cell wall and responsible for the observed effect. The solvent selectivity of the changes points to a laser induced polymerization process. The results emphasize how crucial careful adjustment of experimental parameters in Raman imaging of biological materials is and show the potential of time series and repeated imaging to get additional insights (e.g. monolignols).
format Online
Article
Text
id pubmed-6081397
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-60813972018-08-10 Following laser induced changes of plant phenylpropanoids by Raman microscopy Prats-Mateu, Batirtze Bock, Peter Schroffenegger, Martina Toca-Herrera, José Luis Gierlinger, Notburga Sci Rep Article Raman microscopy is a powerful imaging technique for biological materials providing information about chemistry in context with microstructure. A 532 nm laser is often used as excitation source, because high spatial resolution and signal intensity can be achieved. The latter can be controlled by laser power and integration time, whereby high power and long times give good signal to noise ratio. However, most biological materials absorb in the VIS range and fluorescence masking the signal or even sample degradation might be hindering. Here, we show that on lignified plant cell walls even very short integration times and low laser powers induce a change in the ratio of the lignin bands at 1660 and 1600 cm(−1). Time series on lignin model compounds revealed this change only in aromatic molecules with two OH-groups, such as coniferyl alcohol. Therefore, we conclude that monolignols are present in the cell wall and responsible for the observed effect. The solvent selectivity of the changes points to a laser induced polymerization process. The results emphasize how crucial careful adjustment of experimental parameters in Raman imaging of biological materials is and show the potential of time series and repeated imaging to get additional insights (e.g. monolignols). Nature Publishing Group UK 2018-08-07 /pmc/articles/PMC6081397/ /pubmed/30087373 http://dx.doi.org/10.1038/s41598-018-30096-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Prats-Mateu, Batirtze
Bock, Peter
Schroffenegger, Martina
Toca-Herrera, José Luis
Gierlinger, Notburga
Following laser induced changes of plant phenylpropanoids by Raman microscopy
title Following laser induced changes of plant phenylpropanoids by Raman microscopy
title_full Following laser induced changes of plant phenylpropanoids by Raman microscopy
title_fullStr Following laser induced changes of plant phenylpropanoids by Raman microscopy
title_full_unstemmed Following laser induced changes of plant phenylpropanoids by Raman microscopy
title_short Following laser induced changes of plant phenylpropanoids by Raman microscopy
title_sort following laser induced changes of plant phenylpropanoids by raman microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6081397/
https://www.ncbi.nlm.nih.gov/pubmed/30087373
http://dx.doi.org/10.1038/s41598-018-30096-3
work_keys_str_mv AT pratsmateubatirtze followinglaserinducedchangesofplantphenylpropanoidsbyramanmicroscopy
AT bockpeter followinglaserinducedchangesofplantphenylpropanoidsbyramanmicroscopy
AT schroffeneggermartina followinglaserinducedchangesofplantphenylpropanoidsbyramanmicroscopy
AT tocaherrerajoseluis followinglaserinducedchangesofplantphenylpropanoidsbyramanmicroscopy
AT gierlingernotburga followinglaserinducedchangesofplantphenylpropanoidsbyramanmicroscopy