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Following laser induced changes of plant phenylpropanoids by Raman microscopy
Raman microscopy is a powerful imaging technique for biological materials providing information about chemistry in context with microstructure. A 532 nm laser is often used as excitation source, because high spatial resolution and signal intensity can be achieved. The latter can be controlled by las...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6081397/ https://www.ncbi.nlm.nih.gov/pubmed/30087373 http://dx.doi.org/10.1038/s41598-018-30096-3 |
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author | Prats-Mateu, Batirtze Bock, Peter Schroffenegger, Martina Toca-Herrera, José Luis Gierlinger, Notburga |
author_facet | Prats-Mateu, Batirtze Bock, Peter Schroffenegger, Martina Toca-Herrera, José Luis Gierlinger, Notburga |
author_sort | Prats-Mateu, Batirtze |
collection | PubMed |
description | Raman microscopy is a powerful imaging technique for biological materials providing information about chemistry in context with microstructure. A 532 nm laser is often used as excitation source, because high spatial resolution and signal intensity can be achieved. The latter can be controlled by laser power and integration time, whereby high power and long times give good signal to noise ratio. However, most biological materials absorb in the VIS range and fluorescence masking the signal or even sample degradation might be hindering. Here, we show that on lignified plant cell walls even very short integration times and low laser powers induce a change in the ratio of the lignin bands at 1660 and 1600 cm(−1). Time series on lignin model compounds revealed this change only in aromatic molecules with two OH-groups, such as coniferyl alcohol. Therefore, we conclude that monolignols are present in the cell wall and responsible for the observed effect. The solvent selectivity of the changes points to a laser induced polymerization process. The results emphasize how crucial careful adjustment of experimental parameters in Raman imaging of biological materials is and show the potential of time series and repeated imaging to get additional insights (e.g. monolignols). |
format | Online Article Text |
id | pubmed-6081397 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60813972018-08-10 Following laser induced changes of plant phenylpropanoids by Raman microscopy Prats-Mateu, Batirtze Bock, Peter Schroffenegger, Martina Toca-Herrera, José Luis Gierlinger, Notburga Sci Rep Article Raman microscopy is a powerful imaging technique for biological materials providing information about chemistry in context with microstructure. A 532 nm laser is often used as excitation source, because high spatial resolution and signal intensity can be achieved. The latter can be controlled by laser power and integration time, whereby high power and long times give good signal to noise ratio. However, most biological materials absorb in the VIS range and fluorescence masking the signal or even sample degradation might be hindering. Here, we show that on lignified plant cell walls even very short integration times and low laser powers induce a change in the ratio of the lignin bands at 1660 and 1600 cm(−1). Time series on lignin model compounds revealed this change only in aromatic molecules with two OH-groups, such as coniferyl alcohol. Therefore, we conclude that monolignols are present in the cell wall and responsible for the observed effect. The solvent selectivity of the changes points to a laser induced polymerization process. The results emphasize how crucial careful adjustment of experimental parameters in Raman imaging of biological materials is and show the potential of time series and repeated imaging to get additional insights (e.g. monolignols). Nature Publishing Group UK 2018-08-07 /pmc/articles/PMC6081397/ /pubmed/30087373 http://dx.doi.org/10.1038/s41598-018-30096-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Prats-Mateu, Batirtze Bock, Peter Schroffenegger, Martina Toca-Herrera, José Luis Gierlinger, Notburga Following laser induced changes of plant phenylpropanoids by Raman microscopy |
title | Following laser induced changes of plant phenylpropanoids by Raman microscopy |
title_full | Following laser induced changes of plant phenylpropanoids by Raman microscopy |
title_fullStr | Following laser induced changes of plant phenylpropanoids by Raman microscopy |
title_full_unstemmed | Following laser induced changes of plant phenylpropanoids by Raman microscopy |
title_short | Following laser induced changes of plant phenylpropanoids by Raman microscopy |
title_sort | following laser induced changes of plant phenylpropanoids by raman microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6081397/ https://www.ncbi.nlm.nih.gov/pubmed/30087373 http://dx.doi.org/10.1038/s41598-018-30096-3 |
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