Cargando…
In situ viscoelastic properties and chain conformations of heavily hydrated carboxymethyl dextran layers: a comparative study using OWLS and QCM-I chips coated with waveguide material
Hydration, viscoelastic properties and dominant structure of thin polymer layers on the surface of waveguide material were evaluated using optical waveguide lightmode spectroscopy (OWLS) and quartz crystal microbalance (QCM) methods. The fundamentally different principles of the two applied label-fr...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6081421/ https://www.ncbi.nlm.nih.gov/pubmed/30087383 http://dx.doi.org/10.1038/s41598-018-30201-6 |
_version_ | 1783345645554237440 |
---|---|
author | Saftics, Andras Prósz, György Aurél Türk, Barbara Peter, Beatrix Kurunczi, Sándor Horvath, Robert |
author_facet | Saftics, Andras Prósz, György Aurél Türk, Barbara Peter, Beatrix Kurunczi, Sándor Horvath, Robert |
author_sort | Saftics, Andras |
collection | PubMed |
description | Hydration, viscoelastic properties and dominant structure of thin polymer layers on the surface of waveguide material were evaluated using optical waveguide lightmode spectroscopy (OWLS) and quartz crystal microbalance (QCM) methods. The fundamentally different principles of the two applied label-free biosensors enable to examine analyte layers from complementary aspects, e.g. to determine the amount of bound water in hydrated layers. In this study, a new QCM instrument with impedance measurement (QCM-I) is introduced. Its specially designed sensor chips, covered by thin film of waveguide material, supply identical surface as used in OWLS sensors, thus enabling to perform parallel measurements on the same type of surface. Viscoelastic analysis of the measured data was performed by our evaluation code developed in MATLAB environment, using the Voinova’s Voigt-based model. In situ deposition experiments on the ultrathin films of poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) were conducted for instrumental and code validation. Additionally, a novel OWLS-QCM data evaluation methodology has been developed based on the concept of combining hydration and viscoelastic data with optical anisotropy results from OWLS measurements. This methodology provided insight into the time-dependent chain conformation of heavily hydrated nano-scaled layers, resulting in unprecedented structural, hydration and viscoelastic information on covalently grafted ultrathin carboxymethyl dextran (CMD) films. The measured mass values as well as hydration and viscoelastic properties were compared with the characteristics of PLL-g-PEG layers. |
format | Online Article Text |
id | pubmed-6081421 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60814212018-08-10 In situ viscoelastic properties and chain conformations of heavily hydrated carboxymethyl dextran layers: a comparative study using OWLS and QCM-I chips coated with waveguide material Saftics, Andras Prósz, György Aurél Türk, Barbara Peter, Beatrix Kurunczi, Sándor Horvath, Robert Sci Rep Article Hydration, viscoelastic properties and dominant structure of thin polymer layers on the surface of waveguide material were evaluated using optical waveguide lightmode spectroscopy (OWLS) and quartz crystal microbalance (QCM) methods. The fundamentally different principles of the two applied label-free biosensors enable to examine analyte layers from complementary aspects, e.g. to determine the amount of bound water in hydrated layers. In this study, a new QCM instrument with impedance measurement (QCM-I) is introduced. Its specially designed sensor chips, covered by thin film of waveguide material, supply identical surface as used in OWLS sensors, thus enabling to perform parallel measurements on the same type of surface. Viscoelastic analysis of the measured data was performed by our evaluation code developed in MATLAB environment, using the Voinova’s Voigt-based model. In situ deposition experiments on the ultrathin films of poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) were conducted for instrumental and code validation. Additionally, a novel OWLS-QCM data evaluation methodology has been developed based on the concept of combining hydration and viscoelastic data with optical anisotropy results from OWLS measurements. This methodology provided insight into the time-dependent chain conformation of heavily hydrated nano-scaled layers, resulting in unprecedented structural, hydration and viscoelastic information on covalently grafted ultrathin carboxymethyl dextran (CMD) films. The measured mass values as well as hydration and viscoelastic properties were compared with the characteristics of PLL-g-PEG layers. Nature Publishing Group UK 2018-08-07 /pmc/articles/PMC6081421/ /pubmed/30087383 http://dx.doi.org/10.1038/s41598-018-30201-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Saftics, Andras Prósz, György Aurél Türk, Barbara Peter, Beatrix Kurunczi, Sándor Horvath, Robert In situ viscoelastic properties and chain conformations of heavily hydrated carboxymethyl dextran layers: a comparative study using OWLS and QCM-I chips coated with waveguide material |
title | In situ viscoelastic properties and chain conformations of heavily hydrated carboxymethyl dextran layers: a comparative study using OWLS and QCM-I chips coated with waveguide material |
title_full | In situ viscoelastic properties and chain conformations of heavily hydrated carboxymethyl dextran layers: a comparative study using OWLS and QCM-I chips coated with waveguide material |
title_fullStr | In situ viscoelastic properties and chain conformations of heavily hydrated carboxymethyl dextran layers: a comparative study using OWLS and QCM-I chips coated with waveguide material |
title_full_unstemmed | In situ viscoelastic properties and chain conformations of heavily hydrated carboxymethyl dextran layers: a comparative study using OWLS and QCM-I chips coated with waveguide material |
title_short | In situ viscoelastic properties and chain conformations of heavily hydrated carboxymethyl dextran layers: a comparative study using OWLS and QCM-I chips coated with waveguide material |
title_sort | in situ viscoelastic properties and chain conformations of heavily hydrated carboxymethyl dextran layers: a comparative study using owls and qcm-i chips coated with waveguide material |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6081421/ https://www.ncbi.nlm.nih.gov/pubmed/30087383 http://dx.doi.org/10.1038/s41598-018-30201-6 |
work_keys_str_mv | AT safticsandras insituviscoelasticpropertiesandchainconformationsofheavilyhydratedcarboxymethyldextranlayersacomparativestudyusingowlsandqcmichipscoatedwithwaveguidematerial AT proszgyorgyaurel insituviscoelasticpropertiesandchainconformationsofheavilyhydratedcarboxymethyldextranlayersacomparativestudyusingowlsandqcmichipscoatedwithwaveguidematerial AT turkbarbara insituviscoelasticpropertiesandchainconformationsofheavilyhydratedcarboxymethyldextranlayersacomparativestudyusingowlsandqcmichipscoatedwithwaveguidematerial AT peterbeatrix insituviscoelasticpropertiesandchainconformationsofheavilyhydratedcarboxymethyldextranlayersacomparativestudyusingowlsandqcmichipscoatedwithwaveguidematerial AT kurunczisandor insituviscoelasticpropertiesandchainconformationsofheavilyhydratedcarboxymethyldextranlayersacomparativestudyusingowlsandqcmichipscoatedwithwaveguidematerial AT horvathrobert insituviscoelasticpropertiesandchainconformationsofheavilyhydratedcarboxymethyldextranlayersacomparativestudyusingowlsandqcmichipscoatedwithwaveguidematerial |