Design and MinION testing of a nanopore targeted gene sequencing panel for chronic lymphocytic leukemia

We report a customized gene panel assay based on multiplex long-PCR followed by third generation sequencing on nanopore technology (MinION), designed to analyze five frequently mutated genes in chronic lymphocytic leukemia (CLL): TP53, NOTCH1, BIRC3, SF3B1 and MYD88. For this purpose, 12 patients we...

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Detalles Bibliográficos
Autores principales: Orsini, Paola, Minervini, Crescenzio F., Cumbo, Cosimo, Anelli, Luisa, Zagaria, Antonella, Minervini, Angela, Coccaro, Nicoletta, Tota, Giuseppina, Casieri, Paola, Impera, Luciana, Parciante, Elisa, Brunetti, Claudia, Giordano, Annamaria, Specchia, Giorgina, Albano, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6081477/
https://www.ncbi.nlm.nih.gov/pubmed/30087429
http://dx.doi.org/10.1038/s41598-018-30330-y

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