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Molecular survey of piroplasm species from selected areas of China and Pakistan

BACKGROUND: Piroplasmosis is an important animal disease that is a major constraint to the development of the livestock industry, often resulting in significant economic losses. Therefore, there is an urgent need to further understand the etiology of this and other tick-borne infections. METHODS: Bl...

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Autores principales: Hassan, Muhammad Adeel, Liu, Junlong, Rashid, Muhammad, Iqbal, Naveed, Guan, Guiquan, Yin, Hong, Luo, Jianxun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6081881/
https://www.ncbi.nlm.nih.gov/pubmed/30086785
http://dx.doi.org/10.1186/s13071-018-3035-x
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author Hassan, Muhammad Adeel
Liu, Junlong
Rashid, Muhammad
Iqbal, Naveed
Guan, Guiquan
Yin, Hong
Luo, Jianxun
author_facet Hassan, Muhammad Adeel
Liu, Junlong
Rashid, Muhammad
Iqbal, Naveed
Guan, Guiquan
Yin, Hong
Luo, Jianxun
author_sort Hassan, Muhammad Adeel
collection PubMed
description BACKGROUND: Piroplasmosis is an important animal disease that is a major constraint to the development of the livestock industry, often resulting in significant economic losses. Therefore, there is an urgent need to further understand the etiology of this and other tick-borne infections. METHODS: Blood samples were collected from tick-infested animals from the Chakwal, Jhang, and Faisalabad districts of Punjab, Pakistan and from peri-urban areas around Hohhot, Inner Mongolia, China to investigate the presence of Babesia and Theileria species. In total, 450 blood samples were collected with FTA cards from cattle of the study areas of Pakistan; the genomic (g)DNA of one hundred and twenty samples from cattle in Inner Mongolia were provided by the Lanzhou Veterinary Research Institute, China. Following the extraction of gDNA, the 18S rRNA gene (V4 hypervariable region) of piroplasms was amplified in all samples using semi-nested PCR. Positively identified samples were sequenced for the identification of Theileria and Babesia species. The partial full-length sequence of 18S rDNA was amplified for species confirmation of Theileria-positive samples, whereas the RAP-1c gene was amplified for Babesia bigemina-positive samples. RESULTS: Semi-nested PCR results revealed that 144 (25.26%) samples were positive for piroplasms. Theileria annulata was the most prevalent species (115/144; 20.17%), followed by Theileria orientalis (16/144; 2.80%). Among Babesia, the only species recorded was Babesia bigemina (13/144; 2.28%). CONCLUSION: The present study reveals new data on the prevalence of piroplasm species in bovine populations of selected areas of China and Pakistan and their phylogenetic relationships. It is also the first detailed report of T. orientalis from native animals in Pakistan.
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spelling pubmed-60818812018-08-10 Molecular survey of piroplasm species from selected areas of China and Pakistan Hassan, Muhammad Adeel Liu, Junlong Rashid, Muhammad Iqbal, Naveed Guan, Guiquan Yin, Hong Luo, Jianxun Parasit Vectors Research BACKGROUND: Piroplasmosis is an important animal disease that is a major constraint to the development of the livestock industry, often resulting in significant economic losses. Therefore, there is an urgent need to further understand the etiology of this and other tick-borne infections. METHODS: Blood samples were collected from tick-infested animals from the Chakwal, Jhang, and Faisalabad districts of Punjab, Pakistan and from peri-urban areas around Hohhot, Inner Mongolia, China to investigate the presence of Babesia and Theileria species. In total, 450 blood samples were collected with FTA cards from cattle of the study areas of Pakistan; the genomic (g)DNA of one hundred and twenty samples from cattle in Inner Mongolia were provided by the Lanzhou Veterinary Research Institute, China. Following the extraction of gDNA, the 18S rRNA gene (V4 hypervariable region) of piroplasms was amplified in all samples using semi-nested PCR. Positively identified samples were sequenced for the identification of Theileria and Babesia species. The partial full-length sequence of 18S rDNA was amplified for species confirmation of Theileria-positive samples, whereas the RAP-1c gene was amplified for Babesia bigemina-positive samples. RESULTS: Semi-nested PCR results revealed that 144 (25.26%) samples were positive for piroplasms. Theileria annulata was the most prevalent species (115/144; 20.17%), followed by Theileria orientalis (16/144; 2.80%). Among Babesia, the only species recorded was Babesia bigemina (13/144; 2.28%). CONCLUSION: The present study reveals new data on the prevalence of piroplasm species in bovine populations of selected areas of China and Pakistan and their phylogenetic relationships. It is also the first detailed report of T. orientalis from native animals in Pakistan. BioMed Central 2018-08-07 /pmc/articles/PMC6081881/ /pubmed/30086785 http://dx.doi.org/10.1186/s13071-018-3035-x Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Hassan, Muhammad Adeel
Liu, Junlong
Rashid, Muhammad
Iqbal, Naveed
Guan, Guiquan
Yin, Hong
Luo, Jianxun
Molecular survey of piroplasm species from selected areas of China and Pakistan
title Molecular survey of piroplasm species from selected areas of China and Pakistan
title_full Molecular survey of piroplasm species from selected areas of China and Pakistan
title_fullStr Molecular survey of piroplasm species from selected areas of China and Pakistan
title_full_unstemmed Molecular survey of piroplasm species from selected areas of China and Pakistan
title_short Molecular survey of piroplasm species from selected areas of China and Pakistan
title_sort molecular survey of piroplasm species from selected areas of china and pakistan
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6081881/
https://www.ncbi.nlm.nih.gov/pubmed/30086785
http://dx.doi.org/10.1186/s13071-018-3035-x
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