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Isolation of dioscin-related steroidal saponin from the bulbs of Allium paradoxum L. with leishmanicidal activity
Alliums are rich sources of steroidal saponins, flavonoids, and sulphoric compounds of which steroidal saponins have recently received more attention due to their important pharmacological activities. Allium paradoxum L. is a common edible vegetable in north regions of Iran, especially in Mazandaran...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6082028/ https://www.ncbi.nlm.nih.gov/pubmed/30271449 http://dx.doi.org/10.4103/1735-5362.236875 |
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author | Rezaee, Fatemeh Zolfaghari, Behzad Dinani, Masoud Sadeghi |
author_facet | Rezaee, Fatemeh Zolfaghari, Behzad Dinani, Masoud Sadeghi |
author_sort | Rezaee, Fatemeh |
collection | PubMed |
description | Alliums are rich sources of steroidal saponins, flavonoids, and sulphoric compounds of which steroidal saponins have recently received more attention due to their important pharmacological activities. Allium paradoxum L. is a common edible vegetable in north regions of Iran, especially in Mazandaran province, where it is named “Alezi” and considerably used as a raw vegetable, to make dishes, and as a medicinal plant. Phytochemical investigation of chloroform-methanol extract of the plant resulted in the isolation and identification of a dioscin related steroidal saponin, using comprehensive spectroscopic methods including 1D and 2D NMR, its chemical structure was determined as (25R)-spirost-5-en-3b-ol,3-O-α-rhamnopyranosyl-(1→4)-α-rhamnopyranosyl-(1→4)-[a-rhamnopyranosyl-(1→2)]-glucopyranoside. Investigation of in vitro antileishmanial activity of the isolated compound, in 10 and 50 μg/mL concentrations, exhibited significant leishmanicidal effects (P < 0.001) against the promastigotes of Leishmania major. The results established a valuable basis for further studies about A. paradoxum and anti-parasitic activity of steroidal saponins. |
format | Online Article Text |
id | pubmed-6082028 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-60820282018-10-01 Isolation of dioscin-related steroidal saponin from the bulbs of Allium paradoxum L. with leishmanicidal activity Rezaee, Fatemeh Zolfaghari, Behzad Dinani, Masoud Sadeghi Res Pharm Sci Original Article Alliums are rich sources of steroidal saponins, flavonoids, and sulphoric compounds of which steroidal saponins have recently received more attention due to their important pharmacological activities. Allium paradoxum L. is a common edible vegetable in north regions of Iran, especially in Mazandaran province, where it is named “Alezi” and considerably used as a raw vegetable, to make dishes, and as a medicinal plant. Phytochemical investigation of chloroform-methanol extract of the plant resulted in the isolation and identification of a dioscin related steroidal saponin, using comprehensive spectroscopic methods including 1D and 2D NMR, its chemical structure was determined as (25R)-spirost-5-en-3b-ol,3-O-α-rhamnopyranosyl-(1→4)-α-rhamnopyranosyl-(1→4)-[a-rhamnopyranosyl-(1→2)]-glucopyranoside. Investigation of in vitro antileishmanial activity of the isolated compound, in 10 and 50 μg/mL concentrations, exhibited significant leishmanicidal effects (P < 0.001) against the promastigotes of Leishmania major. The results established a valuable basis for further studies about A. paradoxum and anti-parasitic activity of steroidal saponins. Medknow Publications & Media Pvt Ltd 2018-10 /pmc/articles/PMC6082028/ /pubmed/30271449 http://dx.doi.org/10.4103/1735-5362.236875 Text en Copyright: © 2018 Research in Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Rezaee, Fatemeh Zolfaghari, Behzad Dinani, Masoud Sadeghi Isolation of dioscin-related steroidal saponin from the bulbs of Allium paradoxum L. with leishmanicidal activity |
title | Isolation of dioscin-related steroidal saponin from the bulbs of Allium paradoxum L. with leishmanicidal activity |
title_full | Isolation of dioscin-related steroidal saponin from the bulbs of Allium paradoxum L. with leishmanicidal activity |
title_fullStr | Isolation of dioscin-related steroidal saponin from the bulbs of Allium paradoxum L. with leishmanicidal activity |
title_full_unstemmed | Isolation of dioscin-related steroidal saponin from the bulbs of Allium paradoxum L. with leishmanicidal activity |
title_short | Isolation of dioscin-related steroidal saponin from the bulbs of Allium paradoxum L. with leishmanicidal activity |
title_sort | isolation of dioscin-related steroidal saponin from the bulbs of allium paradoxum l. with leishmanicidal activity |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6082028/ https://www.ncbi.nlm.nih.gov/pubmed/30271449 http://dx.doi.org/10.4103/1735-5362.236875 |
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