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Progress in Circulating Tumor Cell Research Using Microfluidic Devices

Circulating tumor cells (CTCs) are a popular topic in cancer research because they can be obtained by liquid biopsy, a minimally invasive procedure with more sample accessibility than tissue biopsy, to monitor a patient’s condition. Over the past decades, CTC research has covered a wide variety of t...

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Autores principales: Gwak, Hogyeong, Kim, Junmoo, Kashefi-Kheyrabadi, Leila, Kwak, Bongseop, Hyun, Kyung-A, Jung, Hyo-Il
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6082257/
https://www.ncbi.nlm.nih.gov/pubmed/30424286
http://dx.doi.org/10.3390/mi9070353
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author Gwak, Hogyeong
Kim, Junmoo
Kashefi-Kheyrabadi, Leila
Kwak, Bongseop
Hyun, Kyung-A
Jung, Hyo-Il
author_facet Gwak, Hogyeong
Kim, Junmoo
Kashefi-Kheyrabadi, Leila
Kwak, Bongseop
Hyun, Kyung-A
Jung, Hyo-Il
author_sort Gwak, Hogyeong
collection PubMed
description Circulating tumor cells (CTCs) are a popular topic in cancer research because they can be obtained by liquid biopsy, a minimally invasive procedure with more sample accessibility than tissue biopsy, to monitor a patient’s condition. Over the past decades, CTC research has covered a wide variety of topics such as enumeration, profiling, and correlation between CTC number and patient overall survival. It is important to isolate and enrich CTCs before performing CTC analysis because CTCs in the blood stream are very rare (0–10 CTCs/mL of blood). Among the various approaches to separating CTCs, here, we review the research trends in the isolation and analysis of CTCs using microfluidics. Microfluidics provides many attractive advantages for CTC studies such as continuous sample processing to reduce target cell loss and easy integration of various functions into a chip, making “do-everything-on-a-chip” possible. However, tumor cells obtained from different sites within a tumor exhibit heterogenetic features. Thus, heterogeneous CTC profiling should be conducted at a single-cell level after isolation to guide the optimal therapeutic path. We describe the studies on single-CTC analysis based on microfluidic devices. Additionally, as a critical concern in CTC studies, we explain the use of CTCs in cancer research, despite their rarity and heterogeneity, compared with other currently emerging circulating biomarkers, including exosomes and cell-free DNA (cfDNA). Finally, the commercialization of products for CTC separation and analysis is discussed.
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spelling pubmed-60822572018-11-01 Progress in Circulating Tumor Cell Research Using Microfluidic Devices Gwak, Hogyeong Kim, Junmoo Kashefi-Kheyrabadi, Leila Kwak, Bongseop Hyun, Kyung-A Jung, Hyo-Il Micromachines (Basel) Review Circulating tumor cells (CTCs) are a popular topic in cancer research because they can be obtained by liquid biopsy, a minimally invasive procedure with more sample accessibility than tissue biopsy, to monitor a patient’s condition. Over the past decades, CTC research has covered a wide variety of topics such as enumeration, profiling, and correlation between CTC number and patient overall survival. It is important to isolate and enrich CTCs before performing CTC analysis because CTCs in the blood stream are very rare (0–10 CTCs/mL of blood). Among the various approaches to separating CTCs, here, we review the research trends in the isolation and analysis of CTCs using microfluidics. Microfluidics provides many attractive advantages for CTC studies such as continuous sample processing to reduce target cell loss and easy integration of various functions into a chip, making “do-everything-on-a-chip” possible. However, tumor cells obtained from different sites within a tumor exhibit heterogenetic features. Thus, heterogeneous CTC profiling should be conducted at a single-cell level after isolation to guide the optimal therapeutic path. We describe the studies on single-CTC analysis based on microfluidic devices. Additionally, as a critical concern in CTC studies, we explain the use of CTCs in cancer research, despite their rarity and heterogeneity, compared with other currently emerging circulating biomarkers, including exosomes and cell-free DNA (cfDNA). Finally, the commercialization of products for CTC separation and analysis is discussed. MDPI 2018-07-14 /pmc/articles/PMC6082257/ /pubmed/30424286 http://dx.doi.org/10.3390/mi9070353 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Gwak, Hogyeong
Kim, Junmoo
Kashefi-Kheyrabadi, Leila
Kwak, Bongseop
Hyun, Kyung-A
Jung, Hyo-Il
Progress in Circulating Tumor Cell Research Using Microfluidic Devices
title Progress in Circulating Tumor Cell Research Using Microfluidic Devices
title_full Progress in Circulating Tumor Cell Research Using Microfluidic Devices
title_fullStr Progress in Circulating Tumor Cell Research Using Microfluidic Devices
title_full_unstemmed Progress in Circulating Tumor Cell Research Using Microfluidic Devices
title_short Progress in Circulating Tumor Cell Research Using Microfluidic Devices
title_sort progress in circulating tumor cell research using microfluidic devices
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6082257/
https://www.ncbi.nlm.nih.gov/pubmed/30424286
http://dx.doi.org/10.3390/mi9070353
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