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Effect of Poly(Lactide-Co-Glycolide) Nanoparticles on Local Retention of Fluorescent Material: An Experimental Study in Mice

OBJECTIVE: Poly(lactide-co-glycolide) (PLGA) nanoparticles are promising materials for the development of new drug-releasing systems. The purpose of this study was to evaluate the in vivo retention time of materials loaded in nanoparticles as compared with that of the material alone by in vivo imagi...

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Autores principales: Kang, Yeonah, Lee, Eugene, Lee, Joon Woo, Kim, Sung Rae, Kang, Myung Joo, Choi, Young Wook, Ahn, Joong Mo, Kang, Yusuhn, Kang, Heung Sik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Radiology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6082767/
https://www.ncbi.nlm.nih.gov/pubmed/30174485
http://dx.doi.org/10.3348/kjr.2018.19.5.950
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author Kang, Yeonah
Lee, Eugene
Lee, Joon Woo
Kim, Sung Rae
Kang, Myung Joo
Choi, Young Wook
Ahn, Joong Mo
Kang, Yusuhn
Kang, Heung Sik
author_facet Kang, Yeonah
Lee, Eugene
Lee, Joon Woo
Kim, Sung Rae
Kang, Myung Joo
Choi, Young Wook
Ahn, Joong Mo
Kang, Yusuhn
Kang, Heung Sik
author_sort Kang, Yeonah
collection PubMed
description OBJECTIVE: Poly(lactide-co-glycolide) (PLGA) nanoparticles are promising materials for the development of new drug-releasing systems. The purpose of this study was to evaluate the in vivo retention time of materials loaded in nanoparticles as compared with that of the material alone by in vivo imaging in nude mice. MATERIALS AND METHODS: Mice (n = 20) were injected with 0.1 mL fluorescent material 1,1′-dioctadecyl-3,3,3′,3′ tetramethylindotricarbocyanine iodide (DiR)-loaded PLGA nanoparticles (200 nm) into the right paraspinal muscle, and the same volume of pure DiR solution was injected into the left paraspinal muscle. Fluorescence images were obtained using an in vivo optical imaging system. Fluorescent images were taken 1 day after the injection, and seven more images were taken at 1-week intervals. Image analysis was done with ImageJ program, and one region of interest was chosen manually, which corresponded to the highest signal-intensity area of fluorescence signal intensity. RESULTS: After 7 weeks, 12 mice showed a right-sided dominant signal, representing the DiR loaded PLGA nanoparticles; 5 mice showed a left-side dominant signal, representing the free DiR solution; and 3 mice showed no signal at all beginning 1 day after the injection. During the 7-week period, the mean signal intensities of the free DiR solution and DiR-loaded PLGA nanoparticles diverged gradually. On day 1, the mean signal intensity of free DiR solution was significantly higher than that of DiR-loaded PLGA (p < 0.001). Finally, by week 7, DiR-loaded PLGA express significantly high signal intensity compared with free DiR solution (p = 0.031). CONCLUSION: The results of the current study suggested that therapeutic agents bound to PLGA nanoparticles may exhibit prolonged retention times.
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spelling pubmed-60827672018-09-01 Effect of Poly(Lactide-Co-Glycolide) Nanoparticles on Local Retention of Fluorescent Material: An Experimental Study in Mice Kang, Yeonah Lee, Eugene Lee, Joon Woo Kim, Sung Rae Kang, Myung Joo Choi, Young Wook Ahn, Joong Mo Kang, Yusuhn Kang, Heung Sik Korean J Radiol Musculoskeletal Imaging OBJECTIVE: Poly(lactide-co-glycolide) (PLGA) nanoparticles are promising materials for the development of new drug-releasing systems. The purpose of this study was to evaluate the in vivo retention time of materials loaded in nanoparticles as compared with that of the material alone by in vivo imaging in nude mice. MATERIALS AND METHODS: Mice (n = 20) were injected with 0.1 mL fluorescent material 1,1′-dioctadecyl-3,3,3′,3′ tetramethylindotricarbocyanine iodide (DiR)-loaded PLGA nanoparticles (200 nm) into the right paraspinal muscle, and the same volume of pure DiR solution was injected into the left paraspinal muscle. Fluorescence images were obtained using an in vivo optical imaging system. Fluorescent images were taken 1 day after the injection, and seven more images were taken at 1-week intervals. Image analysis was done with ImageJ program, and one region of interest was chosen manually, which corresponded to the highest signal-intensity area of fluorescence signal intensity. RESULTS: After 7 weeks, 12 mice showed a right-sided dominant signal, representing the DiR loaded PLGA nanoparticles; 5 mice showed a left-side dominant signal, representing the free DiR solution; and 3 mice showed no signal at all beginning 1 day after the injection. During the 7-week period, the mean signal intensities of the free DiR solution and DiR-loaded PLGA nanoparticles diverged gradually. On day 1, the mean signal intensity of free DiR solution was significantly higher than that of DiR-loaded PLGA (p < 0.001). Finally, by week 7, DiR-loaded PLGA express significantly high signal intensity compared with free DiR solution (p = 0.031). CONCLUSION: The results of the current study suggested that therapeutic agents bound to PLGA nanoparticles may exhibit prolonged retention times. The Korean Society of Radiology 2018 2018-08-06 /pmc/articles/PMC6082767/ /pubmed/30174485 http://dx.doi.org/10.3348/kjr.2018.19.5.950 Text en Copyright © 2018 The Korean Society of Radiology http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Musculoskeletal Imaging
Kang, Yeonah
Lee, Eugene
Lee, Joon Woo
Kim, Sung Rae
Kang, Myung Joo
Choi, Young Wook
Ahn, Joong Mo
Kang, Yusuhn
Kang, Heung Sik
Effect of Poly(Lactide-Co-Glycolide) Nanoparticles on Local Retention of Fluorescent Material: An Experimental Study in Mice
title Effect of Poly(Lactide-Co-Glycolide) Nanoparticles on Local Retention of Fluorescent Material: An Experimental Study in Mice
title_full Effect of Poly(Lactide-Co-Glycolide) Nanoparticles on Local Retention of Fluorescent Material: An Experimental Study in Mice
title_fullStr Effect of Poly(Lactide-Co-Glycolide) Nanoparticles on Local Retention of Fluorescent Material: An Experimental Study in Mice
title_full_unstemmed Effect of Poly(Lactide-Co-Glycolide) Nanoparticles on Local Retention of Fluorescent Material: An Experimental Study in Mice
title_short Effect of Poly(Lactide-Co-Glycolide) Nanoparticles on Local Retention of Fluorescent Material: An Experimental Study in Mice
title_sort effect of poly(lactide-co-glycolide) nanoparticles on local retention of fluorescent material: an experimental study in mice
topic Musculoskeletal Imaging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6082767/
https://www.ncbi.nlm.nih.gov/pubmed/30174485
http://dx.doi.org/10.3348/kjr.2018.19.5.950
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