Cargando…

Aflatoxin B(1) residues in human livers and their relationship with markers of hepatic carcinogenesis in São Paulo, Brazil

In this study, hepatic biopsies from autopsy cases in São Paulo, Brazil, showing hepatocellular carcinoma (HCC, n = 8), cirrhosis associated with viral hepatitis (VC, n = 20), cirrhosis associated with alcoholism (AC, n = 20), and normal livers (NL or controls, n = 10) were subjected to determinatio...

Descripción completa

Detalles Bibliográficos
Autores principales: Ramalho, Leandra N.Z., Porta, Livia D., Rosim, Roice E., Petta, Tânia, Augusto, Marlei J., Silva, Deisy M., Ramalho, Fernando S., Oliveira, Carlos A.F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6082919/
https://www.ncbi.nlm.nih.gov/pubmed/30101081
http://dx.doi.org/10.1016/j.toxrep.2018.07.005
_version_ 1783345873574428672
author Ramalho, Leandra N.Z.
Porta, Livia D.
Rosim, Roice E.
Petta, Tânia
Augusto, Marlei J.
Silva, Deisy M.
Ramalho, Fernando S.
Oliveira, Carlos A.F.
author_facet Ramalho, Leandra N.Z.
Porta, Livia D.
Rosim, Roice E.
Petta, Tânia
Augusto, Marlei J.
Silva, Deisy M.
Ramalho, Fernando S.
Oliveira, Carlos A.F.
author_sort Ramalho, Leandra N.Z.
collection PubMed
description In this study, hepatic biopsies from autopsy cases in São Paulo, Brazil, showing hepatocellular carcinoma (HCC, n = 8), cirrhosis associated with viral hepatitis (VC, n = 20), cirrhosis associated with alcoholism (AC, n = 20), and normal livers (NL or controls, n = 10) were subjected to determination of aflatoxin B(1) (AFB(1)) and its main metabolites, and of markers of hepatic carcinogenesis Only non-metabolized AFB(1) was detected in 13 samples (27.1%, N = 48) of liver disorders (HCC, VC and AC), at levels between 10.0 and 418.0 pg/g (mean: 76.6 ± 107.7 pg/g). Immuno-labeling of p53, cyclin D1, p21, β-catenin, and Prohibitin (PB) increased mainly in HCC patients, in relation to the controls. AFB(1)+ samples of HCC presented higher expressions of p53, cyclin D1, p21, and β-catenin compared with AFB(1)-livers. In contrast, p27, p16, and Rb immuno-labeling decreased in HCC, VC, and AC samples, compared with NL, with lowest values in AFB(1)+ samples for all liver disorders. Compared with NL, gene expression of cyclin D1 and PB in AFB(1)+ samples of HCC and AC were also higher, along with higher gene expression of p21 in VC and AC AFB(1)+ livers. Results indicated that patients with liver disorders were exposed to dietary aflatoxins, and that residual AFB(1) in liver negatively affected the p53 and protein Rb pathways in HCC. Moreover, the presence of AFB(1) in cirrhotic livers warrants concern about the potential contribution of dietary aflatoxin to disease progression during VC and AC.
format Online
Article
Text
id pubmed-6082919
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-60829192018-08-10 Aflatoxin B(1) residues in human livers and their relationship with markers of hepatic carcinogenesis in São Paulo, Brazil Ramalho, Leandra N.Z. Porta, Livia D. Rosim, Roice E. Petta, Tânia Augusto, Marlei J. Silva, Deisy M. Ramalho, Fernando S. Oliveira, Carlos A.F. Toxicol Rep Article In this study, hepatic biopsies from autopsy cases in São Paulo, Brazil, showing hepatocellular carcinoma (HCC, n = 8), cirrhosis associated with viral hepatitis (VC, n = 20), cirrhosis associated with alcoholism (AC, n = 20), and normal livers (NL or controls, n = 10) were subjected to determination of aflatoxin B(1) (AFB(1)) and its main metabolites, and of markers of hepatic carcinogenesis Only non-metabolized AFB(1) was detected in 13 samples (27.1%, N = 48) of liver disorders (HCC, VC and AC), at levels between 10.0 and 418.0 pg/g (mean: 76.6 ± 107.7 pg/g). Immuno-labeling of p53, cyclin D1, p21, β-catenin, and Prohibitin (PB) increased mainly in HCC patients, in relation to the controls. AFB(1)+ samples of HCC presented higher expressions of p53, cyclin D1, p21, and β-catenin compared with AFB(1)-livers. In contrast, p27, p16, and Rb immuno-labeling decreased in HCC, VC, and AC samples, compared with NL, with lowest values in AFB(1)+ samples for all liver disorders. Compared with NL, gene expression of cyclin D1 and PB in AFB(1)+ samples of HCC and AC were also higher, along with higher gene expression of p21 in VC and AC AFB(1)+ livers. Results indicated that patients with liver disorders were exposed to dietary aflatoxins, and that residual AFB(1) in liver negatively affected the p53 and protein Rb pathways in HCC. Moreover, the presence of AFB(1) in cirrhotic livers warrants concern about the potential contribution of dietary aflatoxin to disease progression during VC and AC. Elsevier 2018-07-25 /pmc/articles/PMC6082919/ /pubmed/30101081 http://dx.doi.org/10.1016/j.toxrep.2018.07.005 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Ramalho, Leandra N.Z.
Porta, Livia D.
Rosim, Roice E.
Petta, Tânia
Augusto, Marlei J.
Silva, Deisy M.
Ramalho, Fernando S.
Oliveira, Carlos A.F.
Aflatoxin B(1) residues in human livers and their relationship with markers of hepatic carcinogenesis in São Paulo, Brazil
title Aflatoxin B(1) residues in human livers and their relationship with markers of hepatic carcinogenesis in São Paulo, Brazil
title_full Aflatoxin B(1) residues in human livers and their relationship with markers of hepatic carcinogenesis in São Paulo, Brazil
title_fullStr Aflatoxin B(1) residues in human livers and their relationship with markers of hepatic carcinogenesis in São Paulo, Brazil
title_full_unstemmed Aflatoxin B(1) residues in human livers and their relationship with markers of hepatic carcinogenesis in São Paulo, Brazil
title_short Aflatoxin B(1) residues in human livers and their relationship with markers of hepatic carcinogenesis in São Paulo, Brazil
title_sort aflatoxin b(1) residues in human livers and their relationship with markers of hepatic carcinogenesis in são paulo, brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6082919/
https://www.ncbi.nlm.nih.gov/pubmed/30101081
http://dx.doi.org/10.1016/j.toxrep.2018.07.005
work_keys_str_mv AT ramalholeandranz aflatoxinb1residuesinhumanliversandtheirrelationshipwithmarkersofhepaticcarcinogenesisinsaopaulobrazil
AT portaliviad aflatoxinb1residuesinhumanliversandtheirrelationshipwithmarkersofhepaticcarcinogenesisinsaopaulobrazil
AT rosimroicee aflatoxinb1residuesinhumanliversandtheirrelationshipwithmarkersofhepaticcarcinogenesisinsaopaulobrazil
AT pettatania aflatoxinb1residuesinhumanliversandtheirrelationshipwithmarkersofhepaticcarcinogenesisinsaopaulobrazil
AT augustomarleij aflatoxinb1residuesinhumanliversandtheirrelationshipwithmarkersofhepaticcarcinogenesisinsaopaulobrazil
AT silvadeisym aflatoxinb1residuesinhumanliversandtheirrelationshipwithmarkersofhepaticcarcinogenesisinsaopaulobrazil
AT ramalhofernandos aflatoxinb1residuesinhumanliversandtheirrelationshipwithmarkersofhepaticcarcinogenesisinsaopaulobrazil
AT oliveiracarlosaf aflatoxinb1residuesinhumanliversandtheirrelationshipwithmarkersofhepaticcarcinogenesisinsaopaulobrazil