Cargando…

Periodontal reconstruction by heparan sulfate mimetic-based matrix therapy in Porphyromonas gingivalis-infected mice

BACKGROUND: Periodontitis is a set of chronic inflammatory diseases affecting the supporting structures of the teeth, during which a persistent release of lytic enzymes and inflammatory mediators causes a self-perpetuating vicious cycle of tissue destruction and repair. A matrix-based therapy using...

Descripción completa

Detalles Bibliográficos
Autores principales: Coyac, Benjamin R., Detzen, Laurent, Doucet, Philippe, Baroukh, Brigitte, Llorens, Annie, Bonnaure-Mallet, Martine, Gosset, Marjolaine, Barritault, Denis, Colombier, Marie-Laure, Saffar, Jean-Louis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6083019/
https://www.ncbi.nlm.nih.gov/pubmed/30101201
http://dx.doi.org/10.1016/j.heliyon.2018.e00719
_version_ 1783345896875884544
author Coyac, Benjamin R.
Detzen, Laurent
Doucet, Philippe
Baroukh, Brigitte
Llorens, Annie
Bonnaure-Mallet, Martine
Gosset, Marjolaine
Barritault, Denis
Colombier, Marie-Laure
Saffar, Jean-Louis
author_facet Coyac, Benjamin R.
Detzen, Laurent
Doucet, Philippe
Baroukh, Brigitte
Llorens, Annie
Bonnaure-Mallet, Martine
Gosset, Marjolaine
Barritault, Denis
Colombier, Marie-Laure
Saffar, Jean-Louis
author_sort Coyac, Benjamin R.
collection PubMed
description BACKGROUND: Periodontitis is a set of chronic inflammatory diseases affecting the supporting structures of the teeth, during which a persistent release of lytic enzymes and inflammatory mediators causes a self-perpetuating vicious cycle of tissue destruction and repair. A matrix-based therapy using a heparan sulfate (HS) analogue called ReGeneraTing Agent (RGTA) replaces destroyed HS by binding to available heparin-binding sites of structural molecules, leading to restoration of tissue homeostasis in several inflammatory tissue injuries, including a hamster periodontitis model. METHODS: The ability of RGTA to restore the periodontium was tested in a model of Porphyromonas gingivalis-infected Balb/cByJ mice. After 12 weeks of disease induction, mice were treated weekly with saline or RGTA (1.5 mg/kg) for 8 weeks. Data were analyzed by histomorphometry. RESULTS: RGTA treatment restored macroscopic bone loss. This was related to (1) a significant reduction in gingival inflammation assessed by a decrease in infiltrated connective tissue, particularly in cells expressing interleukin 1ß, an inflammatory mediator selected as a marker of inflammation; (2) a normalization of bone resorption parameters, i.e. number, activation and activity of osteoclasts, and number of preosteoclasts; (3) a powerful bone formation reaction. The Sharpey's fibers of the periodontal ligament recovered their alkaline phosphatase coating. This was obtained while P. gingivalis infection was maintained throughout the treatment period. CONCLUSIONS: RGTA treatment was able to control the chronic inflammation characteristic of periodontitis and blocked destruction of periodontal structures. It ensured tissue regeneration with recovery of the periodontium's anatomy.
format Online
Article
Text
id pubmed-6083019
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-60830192018-08-10 Periodontal reconstruction by heparan sulfate mimetic-based matrix therapy in Porphyromonas gingivalis-infected mice Coyac, Benjamin R. Detzen, Laurent Doucet, Philippe Baroukh, Brigitte Llorens, Annie Bonnaure-Mallet, Martine Gosset, Marjolaine Barritault, Denis Colombier, Marie-Laure Saffar, Jean-Louis Heliyon Article BACKGROUND: Periodontitis is a set of chronic inflammatory diseases affecting the supporting structures of the teeth, during which a persistent release of lytic enzymes and inflammatory mediators causes a self-perpetuating vicious cycle of tissue destruction and repair. A matrix-based therapy using a heparan sulfate (HS) analogue called ReGeneraTing Agent (RGTA) replaces destroyed HS by binding to available heparin-binding sites of structural molecules, leading to restoration of tissue homeostasis in several inflammatory tissue injuries, including a hamster periodontitis model. METHODS: The ability of RGTA to restore the periodontium was tested in a model of Porphyromonas gingivalis-infected Balb/cByJ mice. After 12 weeks of disease induction, mice were treated weekly with saline or RGTA (1.5 mg/kg) for 8 weeks. Data were analyzed by histomorphometry. RESULTS: RGTA treatment restored macroscopic bone loss. This was related to (1) a significant reduction in gingival inflammation assessed by a decrease in infiltrated connective tissue, particularly in cells expressing interleukin 1ß, an inflammatory mediator selected as a marker of inflammation; (2) a normalization of bone resorption parameters, i.e. number, activation and activity of osteoclasts, and number of preosteoclasts; (3) a powerful bone formation reaction. The Sharpey's fibers of the periodontal ligament recovered their alkaline phosphatase coating. This was obtained while P. gingivalis infection was maintained throughout the treatment period. CONCLUSIONS: RGTA treatment was able to control the chronic inflammation characteristic of periodontitis and blocked destruction of periodontal structures. It ensured tissue regeneration with recovery of the periodontium's anatomy. Elsevier 2018-08-06 /pmc/articles/PMC6083019/ /pubmed/30101201 http://dx.doi.org/10.1016/j.heliyon.2018.e00719 Text en © 2018 The Authors. Published by Elsevier Ltd. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Coyac, Benjamin R.
Detzen, Laurent
Doucet, Philippe
Baroukh, Brigitte
Llorens, Annie
Bonnaure-Mallet, Martine
Gosset, Marjolaine
Barritault, Denis
Colombier, Marie-Laure
Saffar, Jean-Louis
Periodontal reconstruction by heparan sulfate mimetic-based matrix therapy in Porphyromonas gingivalis-infected mice
title Periodontal reconstruction by heparan sulfate mimetic-based matrix therapy in Porphyromonas gingivalis-infected mice
title_full Periodontal reconstruction by heparan sulfate mimetic-based matrix therapy in Porphyromonas gingivalis-infected mice
title_fullStr Periodontal reconstruction by heparan sulfate mimetic-based matrix therapy in Porphyromonas gingivalis-infected mice
title_full_unstemmed Periodontal reconstruction by heparan sulfate mimetic-based matrix therapy in Porphyromonas gingivalis-infected mice
title_short Periodontal reconstruction by heparan sulfate mimetic-based matrix therapy in Porphyromonas gingivalis-infected mice
title_sort periodontal reconstruction by heparan sulfate mimetic-based matrix therapy in porphyromonas gingivalis-infected mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6083019/
https://www.ncbi.nlm.nih.gov/pubmed/30101201
http://dx.doi.org/10.1016/j.heliyon.2018.e00719
work_keys_str_mv AT coyacbenjaminr periodontalreconstructionbyheparansulfatemimeticbasedmatrixtherapyinporphyromonasgingivalisinfectedmice
AT detzenlaurent periodontalreconstructionbyheparansulfatemimeticbasedmatrixtherapyinporphyromonasgingivalisinfectedmice
AT doucetphilippe periodontalreconstructionbyheparansulfatemimeticbasedmatrixtherapyinporphyromonasgingivalisinfectedmice
AT baroukhbrigitte periodontalreconstructionbyheparansulfatemimeticbasedmatrixtherapyinporphyromonasgingivalisinfectedmice
AT llorensannie periodontalreconstructionbyheparansulfatemimeticbasedmatrixtherapyinporphyromonasgingivalisinfectedmice
AT bonnauremalletmartine periodontalreconstructionbyheparansulfatemimeticbasedmatrixtherapyinporphyromonasgingivalisinfectedmice
AT gossetmarjolaine periodontalreconstructionbyheparansulfatemimeticbasedmatrixtherapyinporphyromonasgingivalisinfectedmice
AT barritaultdenis periodontalreconstructionbyheparansulfatemimeticbasedmatrixtherapyinporphyromonasgingivalisinfectedmice
AT colombiermarielaure periodontalreconstructionbyheparansulfatemimeticbasedmatrixtherapyinporphyromonasgingivalisinfectedmice
AT saffarjeanlouis periodontalreconstructionbyheparansulfatemimeticbasedmatrixtherapyinporphyromonasgingivalisinfectedmice