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Population Structure, Antimicrobial Resistance, and Virulence-Associated Genes in Campylobacter jejuni Isolated From Three Ecological Niches: Gastroenteritis Patients, Broilers, and Wild Birds

Campylobacter jejuni is the causal agent of the food-borne infection with the highest incidence in Europe. Both poultry and wild birds are a major reservoir. To gain insight into the population structure, virulence potential, and antimicrobial resistance (AMR), a collection of 150 isolates from thre...

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Autores principales: Iglesias-Torrens, Yaidelys, Miró, Elisenda, Guirado, Pedro, Llovet, Teresa, Muñoz, Carmen, Cerdà-Cuéllar, Marta, Madrid, Cristina, Balsalobre, Carlos, Navarro, Ferran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6083060/
https://www.ncbi.nlm.nih.gov/pubmed/30116225
http://dx.doi.org/10.3389/fmicb.2018.01676
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author Iglesias-Torrens, Yaidelys
Miró, Elisenda
Guirado, Pedro
Llovet, Teresa
Muñoz, Carmen
Cerdà-Cuéllar, Marta
Madrid, Cristina
Balsalobre, Carlos
Navarro, Ferran
author_facet Iglesias-Torrens, Yaidelys
Miró, Elisenda
Guirado, Pedro
Llovet, Teresa
Muñoz, Carmen
Cerdà-Cuéllar, Marta
Madrid, Cristina
Balsalobre, Carlos
Navarro, Ferran
author_sort Iglesias-Torrens, Yaidelys
collection PubMed
description Campylobacter jejuni is the causal agent of the food-borne infection with the highest incidence in Europe. Both poultry and wild birds are a major reservoir. To gain insight into the population structure, virulence potential, and antimicrobial resistance (AMR), a collection of 150 isolates from three different ecological niches (broilers, wild birds, and human patients) was studied. Despite the high genetic diversity found, the population structure defined two distinct clusters, one formed mostly by broiler and human isolates and another one by most wild bird isolates. The ST-21 complex exhibits highest prevalence (in humans and broilers), followed by ST-1275 complex (only in wild birds). The ST-48, -45, and -354 complexes were found in all three niches, but represent only 22 out of 150 studied strains. A higher occurrence of AMR and multidrug resistance was detected among broiler and human isolates. Moreover, significant differences were found in the distribution of certain putative virulence genes. Remarkably, many wild bird strains were negative for either cdtA, cdtB, or cdtC from the canonical strain 81-176, whereas all broiler and human strains were positive. These data suggest that the different variants of the cdt genes might be relevant for the efficient colonization of certain hosts by C. jejuni. Our study contributes to the understanding of the role of the diverse Campylobacter reservoirs in the transmission of campylobacteriosis to humans.
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spelling pubmed-60830602018-08-16 Population Structure, Antimicrobial Resistance, and Virulence-Associated Genes in Campylobacter jejuni Isolated From Three Ecological Niches: Gastroenteritis Patients, Broilers, and Wild Birds Iglesias-Torrens, Yaidelys Miró, Elisenda Guirado, Pedro Llovet, Teresa Muñoz, Carmen Cerdà-Cuéllar, Marta Madrid, Cristina Balsalobre, Carlos Navarro, Ferran Front Microbiol Microbiology Campylobacter jejuni is the causal agent of the food-borne infection with the highest incidence in Europe. Both poultry and wild birds are a major reservoir. To gain insight into the population structure, virulence potential, and antimicrobial resistance (AMR), a collection of 150 isolates from three different ecological niches (broilers, wild birds, and human patients) was studied. Despite the high genetic diversity found, the population structure defined two distinct clusters, one formed mostly by broiler and human isolates and another one by most wild bird isolates. The ST-21 complex exhibits highest prevalence (in humans and broilers), followed by ST-1275 complex (only in wild birds). The ST-48, -45, and -354 complexes were found in all three niches, but represent only 22 out of 150 studied strains. A higher occurrence of AMR and multidrug resistance was detected among broiler and human isolates. Moreover, significant differences were found in the distribution of certain putative virulence genes. Remarkably, many wild bird strains were negative for either cdtA, cdtB, or cdtC from the canonical strain 81-176, whereas all broiler and human strains were positive. These data suggest that the different variants of the cdt genes might be relevant for the efficient colonization of certain hosts by C. jejuni. Our study contributes to the understanding of the role of the diverse Campylobacter reservoirs in the transmission of campylobacteriosis to humans. Frontiers Media S.A. 2018-08-02 /pmc/articles/PMC6083060/ /pubmed/30116225 http://dx.doi.org/10.3389/fmicb.2018.01676 Text en Copyright © 2018 Iglesias-Torrens, Miró, Guirado, Llovet, Muñoz, Cerdà-Cuéllar, Madrid, Balsalobre and Navarro. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Iglesias-Torrens, Yaidelys
Miró, Elisenda
Guirado, Pedro
Llovet, Teresa
Muñoz, Carmen
Cerdà-Cuéllar, Marta
Madrid, Cristina
Balsalobre, Carlos
Navarro, Ferran
Population Structure, Antimicrobial Resistance, and Virulence-Associated Genes in Campylobacter jejuni Isolated From Three Ecological Niches: Gastroenteritis Patients, Broilers, and Wild Birds
title Population Structure, Antimicrobial Resistance, and Virulence-Associated Genes in Campylobacter jejuni Isolated From Three Ecological Niches: Gastroenteritis Patients, Broilers, and Wild Birds
title_full Population Structure, Antimicrobial Resistance, and Virulence-Associated Genes in Campylobacter jejuni Isolated From Three Ecological Niches: Gastroenteritis Patients, Broilers, and Wild Birds
title_fullStr Population Structure, Antimicrobial Resistance, and Virulence-Associated Genes in Campylobacter jejuni Isolated From Three Ecological Niches: Gastroenteritis Patients, Broilers, and Wild Birds
title_full_unstemmed Population Structure, Antimicrobial Resistance, and Virulence-Associated Genes in Campylobacter jejuni Isolated From Three Ecological Niches: Gastroenteritis Patients, Broilers, and Wild Birds
title_short Population Structure, Antimicrobial Resistance, and Virulence-Associated Genes in Campylobacter jejuni Isolated From Three Ecological Niches: Gastroenteritis Patients, Broilers, and Wild Birds
title_sort population structure, antimicrobial resistance, and virulence-associated genes in campylobacter jejuni isolated from three ecological niches: gastroenteritis patients, broilers, and wild birds
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6083060/
https://www.ncbi.nlm.nih.gov/pubmed/30116225
http://dx.doi.org/10.3389/fmicb.2018.01676
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