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3D bioprinting of a corneal stroma equivalent
Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. However, successful translation...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Academic Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6083436/ https://www.ncbi.nlm.nih.gov/pubmed/29772228 http://dx.doi.org/10.1016/j.exer.2018.05.010 |
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author | Isaacson, Abigail Swioklo, Stephen Connon, Che J. |
author_facet | Isaacson, Abigail Swioklo, Stephen Connon, Che J. |
author_sort | Isaacson, Abigail |
collection | PubMed |
description | Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. However, successful translation of these therapies into the clinic has not yet been accomplished. 3D bioprinting is an emerging technology that can be harnessed for the fabrication of biological tissue for clinical applications. We applied this to the area of corneal tissue engineering in order to fabricate corneal structures that resembled the structure of the native human corneal stroma using an existing 3D digital human corneal model and a suitable support structure. These were 3D bioprinted from an in-house collagen-based bio-ink containing encapsulated corneal keratocytes. Keratocytes exhibited high cell viability both at day 1 post-printing (>90%) and at day 7 (83%). We established 3D bio-printing to be a feasible method by which artificial corneal structures can be engineered. |
format | Online Article Text |
id | pubmed-6083436 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Academic Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-60834362018-08-10 3D bioprinting of a corneal stroma equivalent Isaacson, Abigail Swioklo, Stephen Connon, Che J. Exp Eye Res Article Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. However, successful translation of these therapies into the clinic has not yet been accomplished. 3D bioprinting is an emerging technology that can be harnessed for the fabrication of biological tissue for clinical applications. We applied this to the area of corneal tissue engineering in order to fabricate corneal structures that resembled the structure of the native human corneal stroma using an existing 3D digital human corneal model and a suitable support structure. These were 3D bioprinted from an in-house collagen-based bio-ink containing encapsulated corneal keratocytes. Keratocytes exhibited high cell viability both at day 1 post-printing (>90%) and at day 7 (83%). We established 3D bio-printing to be a feasible method by which artificial corneal structures can be engineered. Academic Press 2018-08 /pmc/articles/PMC6083436/ /pubmed/29772228 http://dx.doi.org/10.1016/j.exer.2018.05.010 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Isaacson, Abigail Swioklo, Stephen Connon, Che J. 3D bioprinting of a corneal stroma equivalent |
title | 3D bioprinting of a corneal stroma equivalent |
title_full | 3D bioprinting of a corneal stroma equivalent |
title_fullStr | 3D bioprinting of a corneal stroma equivalent |
title_full_unstemmed | 3D bioprinting of a corneal stroma equivalent |
title_short | 3D bioprinting of a corneal stroma equivalent |
title_sort | 3d bioprinting of a corneal stroma equivalent |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6083436/ https://www.ncbi.nlm.nih.gov/pubmed/29772228 http://dx.doi.org/10.1016/j.exer.2018.05.010 |
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