Cargando…

3D bioprinting of a corneal stroma equivalent

Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. However, successful translation...

Descripción completa

Detalles Bibliográficos
Autores principales: Isaacson, Abigail, Swioklo, Stephen, Connon, Che J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6083436/
https://www.ncbi.nlm.nih.gov/pubmed/29772228
http://dx.doi.org/10.1016/j.exer.2018.05.010
_version_ 1783345975055613952
author Isaacson, Abigail
Swioklo, Stephen
Connon, Che J.
author_facet Isaacson, Abigail
Swioklo, Stephen
Connon, Che J.
author_sort Isaacson, Abigail
collection PubMed
description Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. However, successful translation of these therapies into the clinic has not yet been accomplished. 3D bioprinting is an emerging technology that can be harnessed for the fabrication of biological tissue for clinical applications. We applied this to the area of corneal tissue engineering in order to fabricate corneal structures that resembled the structure of the native human corneal stroma using an existing 3D digital human corneal model and a suitable support structure. These were 3D bioprinted from an in-house collagen-based bio-ink containing encapsulated corneal keratocytes. Keratocytes exhibited high cell viability both at day 1 post-printing (>90%) and at day 7 (83%). We established 3D bio-printing to be a feasible method by which artificial corneal structures can be engineered.
format Online
Article
Text
id pubmed-6083436
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Academic Press
record_format MEDLINE/PubMed
spelling pubmed-60834362018-08-10 3D bioprinting of a corneal stroma equivalent Isaacson, Abigail Swioklo, Stephen Connon, Che J. Exp Eye Res Article Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. However, successful translation of these therapies into the clinic has not yet been accomplished. 3D bioprinting is an emerging technology that can be harnessed for the fabrication of biological tissue for clinical applications. We applied this to the area of corneal tissue engineering in order to fabricate corneal structures that resembled the structure of the native human corneal stroma using an existing 3D digital human corneal model and a suitable support structure. These were 3D bioprinted from an in-house collagen-based bio-ink containing encapsulated corneal keratocytes. Keratocytes exhibited high cell viability both at day 1 post-printing (>90%) and at day 7 (83%). We established 3D bio-printing to be a feasible method by which artificial corneal structures can be engineered. Academic Press 2018-08 /pmc/articles/PMC6083436/ /pubmed/29772228 http://dx.doi.org/10.1016/j.exer.2018.05.010 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Isaacson, Abigail
Swioklo, Stephen
Connon, Che J.
3D bioprinting of a corneal stroma equivalent
title 3D bioprinting of a corneal stroma equivalent
title_full 3D bioprinting of a corneal stroma equivalent
title_fullStr 3D bioprinting of a corneal stroma equivalent
title_full_unstemmed 3D bioprinting of a corneal stroma equivalent
title_short 3D bioprinting of a corneal stroma equivalent
title_sort 3d bioprinting of a corneal stroma equivalent
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6083436/
https://www.ncbi.nlm.nih.gov/pubmed/29772228
http://dx.doi.org/10.1016/j.exer.2018.05.010
work_keys_str_mv AT isaacsonabigail 3dbioprintingofacornealstromaequivalent
AT swioklostephen 3dbioprintingofacornealstromaequivalent
AT connonchej 3dbioprintingofacornealstromaequivalent