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ExSTA: External Standard Addition Method for Accurate High‐Throughput Quantitation in Targeted Proteomics Experiments

PURPOSE: Targeted proteomics using MRM with stable‐isotope‐labeled internal‐standard (SIS) peptides is the current method of choice for protein quantitation in complex biological matrices. Better quantitation can be achieved with the internal standard‐addition method, where successive increments of...

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Detalles Bibliográficos
Autores principales: Mohammed, Yassene, Pan, Jingxi, Zhang, Suping, Han, Jun, Borchers, Christoph H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6084352/
https://www.ncbi.nlm.nih.gov/pubmed/28895300
http://dx.doi.org/10.1002/prca.201600180

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