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pH-responsive polymer microcapsules for targeted delivery of biomaterials to the midgut of Drosophila suzukii

Drosophila suzukii or spotted wing Drosophila is an economically important pest which can have a devastating impact on soft and stone fruit industries. Biological pesticides are being sought as alternatives to synthetic chemicals to control this invasive pest, but many are subject to degradation eit...

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Autores principales: Ferguson, Calum T. J., Al-Khalaf, Areej A., Isaac, R. Elwyn, Cayre, Olivier J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6084892/
https://www.ncbi.nlm.nih.gov/pubmed/30091982
http://dx.doi.org/10.1371/journal.pone.0201294
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author Ferguson, Calum T. J.
Al-Khalaf, Areej A.
Isaac, R. Elwyn
Cayre, Olivier J.
author_facet Ferguson, Calum T. J.
Al-Khalaf, Areej A.
Isaac, R. Elwyn
Cayre, Olivier J.
author_sort Ferguson, Calum T. J.
collection PubMed
description Drosophila suzukii or spotted wing Drosophila is an economically important pest which can have a devastating impact on soft and stone fruit industries. Biological pesticides are being sought as alternatives to synthetic chemicals to control this invasive pest, but many are subject to degradation either in the environment or in the insect gut and as a result require protection. In this study we identified a sharp change in pH of the adult midgut from neutral to acidic (pH <3), which we then exploited to develop poly(2-vinylpyridine) (P2VP) microcapsules that respond to the change in midgut pH by dissolution and release of their cargo for uptake into the insect. First, we used labelled solid poly(methyl methacrylate) (PMMA) particles to show that microcapsules with a diameter less than 15 μm are readily ingested by the adult insect. To encapsulate water-soluble biological species in an aqueous continuous phase, a multiple emulsion template was used as a precursor for the synthesis of pH-responsive P2VP microcapsules with a fluorescent (FITC-dextran) cargo. The water-soluble agent was initially separated from the aqueous continuous phase by an oil barrier, which was subsequently polymerised. The P2VP microcapsules were stable at pH > 6, but underwent rapid dissolution at pH < 4.2. In vivo studies showed that the natural acidity of the midgut of D. suzukii also induced the breakdown of the responsive P2VP microcapsules to release FITC-dextran which was taken up into the body of the insect and accumulated in the renal tubules.
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spelling pubmed-60848922018-08-18 pH-responsive polymer microcapsules for targeted delivery of biomaterials to the midgut of Drosophila suzukii Ferguson, Calum T. J. Al-Khalaf, Areej A. Isaac, R. Elwyn Cayre, Olivier J. PLoS One Research Article Drosophila suzukii or spotted wing Drosophila is an economically important pest which can have a devastating impact on soft and stone fruit industries. Biological pesticides are being sought as alternatives to synthetic chemicals to control this invasive pest, but many are subject to degradation either in the environment or in the insect gut and as a result require protection. In this study we identified a sharp change in pH of the adult midgut from neutral to acidic (pH <3), which we then exploited to develop poly(2-vinylpyridine) (P2VP) microcapsules that respond to the change in midgut pH by dissolution and release of their cargo for uptake into the insect. First, we used labelled solid poly(methyl methacrylate) (PMMA) particles to show that microcapsules with a diameter less than 15 μm are readily ingested by the adult insect. To encapsulate water-soluble biological species in an aqueous continuous phase, a multiple emulsion template was used as a precursor for the synthesis of pH-responsive P2VP microcapsules with a fluorescent (FITC-dextran) cargo. The water-soluble agent was initially separated from the aqueous continuous phase by an oil barrier, which was subsequently polymerised. The P2VP microcapsules were stable at pH > 6, but underwent rapid dissolution at pH < 4.2. In vivo studies showed that the natural acidity of the midgut of D. suzukii also induced the breakdown of the responsive P2VP microcapsules to release FITC-dextran which was taken up into the body of the insect and accumulated in the renal tubules. Public Library of Science 2018-08-09 /pmc/articles/PMC6084892/ /pubmed/30091982 http://dx.doi.org/10.1371/journal.pone.0201294 Text en © 2018 Ferguson et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ferguson, Calum T. J.
Al-Khalaf, Areej A.
Isaac, R. Elwyn
Cayre, Olivier J.
pH-responsive polymer microcapsules for targeted delivery of biomaterials to the midgut of Drosophila suzukii
title pH-responsive polymer microcapsules for targeted delivery of biomaterials to the midgut of Drosophila suzukii
title_full pH-responsive polymer microcapsules for targeted delivery of biomaterials to the midgut of Drosophila suzukii
title_fullStr pH-responsive polymer microcapsules for targeted delivery of biomaterials to the midgut of Drosophila suzukii
title_full_unstemmed pH-responsive polymer microcapsules for targeted delivery of biomaterials to the midgut of Drosophila suzukii
title_short pH-responsive polymer microcapsules for targeted delivery of biomaterials to the midgut of Drosophila suzukii
title_sort ph-responsive polymer microcapsules for targeted delivery of biomaterials to the midgut of drosophila suzukii
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6084892/
https://www.ncbi.nlm.nih.gov/pubmed/30091982
http://dx.doi.org/10.1371/journal.pone.0201294
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