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Immunohistochemistry of the circadian clock in mouse and human vascular tissues
AIM: The circadian clock is a molecular network that controls the body physiological rhythms. In blood vessels, the circadian clock components modulate vascular remodeling, blood pressure, and signaling. The goal in this study was to determine the pattern of expression of circadian clock proteins in...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085090/ https://www.ncbi.nlm.nih.gov/pubmed/30101218 http://dx.doi.org/10.20517/2574-1209.2018.46 |
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author | Anea, Ciprian B. Merloiu, Ana M. Fulton, David J. R. Patel, Vijay Rudic, R. Dan |
author_facet | Anea, Ciprian B. Merloiu, Ana M. Fulton, David J. R. Patel, Vijay Rudic, R. Dan |
author_sort | Anea, Ciprian B. |
collection | PubMed |
description | AIM: The circadian clock is a molecular network that controls the body physiological rhythms. In blood vessels, the circadian clock components modulate vascular remodeling, blood pressure, and signaling. The goal in this study was to determine the pattern of expression of circadian clock proteins in the endothelium, smooth muscle, and adventitia of the vasculature of human and mouse tissues. METHODS: Immunohistochemistry was performed in frozen sections of mouse aorta, common carotid artery, femoral artery, lung, and heart at 12 AM and 12 PM for Bmal1, Clock, Npas2, Per and other clock components. Studies of expression were also assessed in human saphenous vein both by immunoblotting and immunohistochemistry. RESULTS: In this study, we identified the expression of Bmal1, Clock, Npas, Per1, Cry1, and accessory clock components by immunohistochemical staining in the endothelium, smooth muscle and adventitia of the mouse vasculature with differing temporal and cellular profiles depending on vasculature and tissue analyzed. The human saphenous vein also exhibited expression of clock genes that exhibited an oscillatory pattern in Bmal1 and Cry by immunoblotting. CONCLUSION: These studies show that circadian clock components display differences in expression and localization throughout the cardiovascular system, which may confer nuances of circadian clock signaling in a cell-specific manner. |
format | Online Article Text |
id | pubmed-6085090 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
record_format | MEDLINE/PubMed |
spelling | pubmed-60850902018-08-09 Immunohistochemistry of the circadian clock in mouse and human vascular tissues Anea, Ciprian B. Merloiu, Ana M. Fulton, David J. R. Patel, Vijay Rudic, R. Dan Vessel Plus Article AIM: The circadian clock is a molecular network that controls the body physiological rhythms. In blood vessels, the circadian clock components modulate vascular remodeling, blood pressure, and signaling. The goal in this study was to determine the pattern of expression of circadian clock proteins in the endothelium, smooth muscle, and adventitia of the vasculature of human and mouse tissues. METHODS: Immunohistochemistry was performed in frozen sections of mouse aorta, common carotid artery, femoral artery, lung, and heart at 12 AM and 12 PM for Bmal1, Clock, Npas2, Per and other clock components. Studies of expression were also assessed in human saphenous vein both by immunoblotting and immunohistochemistry. RESULTS: In this study, we identified the expression of Bmal1, Clock, Npas, Per1, Cry1, and accessory clock components by immunohistochemical staining in the endothelium, smooth muscle and adventitia of the mouse vasculature with differing temporal and cellular profiles depending on vasculature and tissue analyzed. The human saphenous vein also exhibited expression of clock genes that exhibited an oscillatory pattern in Bmal1 and Cry by immunoblotting. CONCLUSION: These studies show that circadian clock components display differences in expression and localization throughout the cardiovascular system, which may confer nuances of circadian clock signaling in a cell-specific manner. 2018-07-20 2018 /pmc/articles/PMC6085090/ /pubmed/30101218 http://dx.doi.org/10.20517/2574-1209.2018.46 Text en https://creativecommons.org/licenses/by/4.0/ This article is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Article Anea, Ciprian B. Merloiu, Ana M. Fulton, David J. R. Patel, Vijay Rudic, R. Dan Immunohistochemistry of the circadian clock in mouse and human vascular tissues |
title | Immunohistochemistry of the circadian clock in mouse and human
vascular tissues |
title_full | Immunohistochemistry of the circadian clock in mouse and human
vascular tissues |
title_fullStr | Immunohistochemistry of the circadian clock in mouse and human
vascular tissues |
title_full_unstemmed | Immunohistochemistry of the circadian clock in mouse and human
vascular tissues |
title_short | Immunohistochemistry of the circadian clock in mouse and human
vascular tissues |
title_sort | immunohistochemistry of the circadian clock in mouse and human
vascular tissues |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085090/ https://www.ncbi.nlm.nih.gov/pubmed/30101218 http://dx.doi.org/10.20517/2574-1209.2018.46 |
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